Combined CSL and p53 Downregulation Promotes Cancer-Associated Fibroblast Activation The authors report that CSL silencing induces senescence of primary fibroblasts from dermis, oral mucosa, breast and lung. CSL functions in these cells as a direct repressor of multiple senescence- and cancer-associated fibroblast-effector genes. [Nat Cell Biol] Abstract Matrix Assisted Transplantation of Functional Beige Adipose Tissue Scientists present a novel cell scaffold technology optimized to establish functional brown fat-like depots in vivo. They adapted the biophysical properties of hyaluronic acid-based hydrogels to support the differentiation of white fat derived multipotent stem cells into lipid accumulating, uncoupling protein 1 expressing beige adipose tissue. [Diabetes] Abstract | Press Release Ultraporous Nanofeatured PCL–PEO Microfibrous Scaffolds Enhance Cell Infiltration, Colonization and Myofibroblastic Differentiation Investigators fabricated hydrophilic, micro-porous scaffolds with nano-topographical cues by one-step electrospinning, and investigated NIH3T3 fibroblasts cell infiltration, colonization and myofibroblastic differentiation. [Nanoscale] Abstract Incorporation of Fibrin into a Collagen-Glycosaminoglycan Matrix Results in a Scaffold with Improved Mechanical Properties and Enhanced Capacity to Resist Cell-Mediated Contraction Through the use of an injection loading technique, a homogenous fibrin, collagen and glycosaminoglycan (FCG) scaffold was developed. Human vascular smooth muscle cells were successfully incorporated into the FCG scaffold and demonstrated excellent viability over seven days, while proliferation of these cells also increased significantly. [Acta Biomater] Abstract Engineered Composite Fascia for Stem Cell Therapy in Tissue Repair Applications Investigators describe a composite, laser micromachined, collagen-alginate construct containing human mesenchymal stem cells (hMSCs) for tissue repair applications. As compared to acellular constructs, constructs containing hMSCs displayed greater integration strength, increased vascularization, and increased infiltration of macrophages. [Acta Biomater] Abstract | Graphical Abstract HCRP-1 Regulates Cell Migration and Invasion via EGFR-ERK Mediated Up-Regulation of MMP-2 with Prognostic Significance in Human Renal Cell Carcinoma Knockdown of hepatocellular carcinoma-related protein-1 (HCRP-1) promoted cell migration and invasion in 786-O and OS-RC-2 cell lines. HCRP-1 depletion increased matrix metalloproteinase (MMP)-2 protein level, with increased extracellular signal-regulated kinase (ERK) phosphorylation, which could be reversed by ERK siRNA or ERK inhibitor, PD98059. [Sci Rep] Full Article A 3-Dimensional Fiber Scaffold as an Investigative Tool for Studying the Morphogenesis of Isolated Plant Pells Researchers have developed a method of three-dimensional cell culture for plants, which mimics the plant tissue environment, using biocompatible scaffolds similar to those used in mammalian tissue engineering. The scaffolds provide both developmental cues and structural stability to isolated callus-derived cells grown in liquid culture. [BMC Plant Biol] Full Article | Press Release Involvement of ZEB1 and Snail1 in Excessive Production of Extracellular Matrix in Fuchs Endothelial Corneal Dystrophy Scientists established a cellular in vitro model by using immortalized corneal endothelial cells obtained from late-onset Fuchs endothelial corneal dystrophy and control patients and examined the involvement of epithelial mesenchymal transition on excessive extracellular matrix production. [Lab Invest] Abstract MicroRNA-148a Inhibits Migration of Breast Cancer Cells by Targeting MMP-13 Researchers investigated the role of microRNA (miR)-148a in migration of breast cancer cells as well as the underlying mechanism. miR-148a was found to inhibit the proliferation and migration of breast cancer cells. [Tumor Biol] Abstract Expanding the Activity of Tissue Inhibitors of Metalloproteinase (TIMP)-1 against Surface-Anchored Metalloproteinases by the Replacement of Its C-Terminal Domain: Implications for Anti-Cancer Effects Scientists replaced the C-terminal domain of TIMP-1 with those of TIMP-2, -3 and -4 to create a series of “T1:TX” chimeras. The affinity of the chimeras against ADAM10, ADAM17, matrix metalloproteinase (MMP)14 and MMP19 was investigated. [PLoS One] Full Article |