A Novel Collagen Matricryptin Reduces Left Ventricular Dilation Post-Myocardial Infarction by Promoting Scar Formation and Angiogenesis Researchers identified novel collagen-derived matricryptins generated post-myocardial infarction that mediate remodeling of the left ventricle. They synthesized a peptide beginning at the cleavage site (p1158/59) to investigate its biological functions. In vitro, p1158/59 stimulated fibroblast wound healing and robustly promoted angiogenesis. [J Am Coll Cardiol] Abstract 3D Printed Anatomical Nerve Regeneration Pathways In vivo studies examining the regeneration of bifurcated injuries across a 10 mm complex nerve gap in rats showed that the 3D printed scaffolds achieved successful regeneration of complex nerve injuries, resulting in enhanced functional return of the regenerated nerve. [Adv Funct Mater] Abstract | Press Release | Video Human Pluripotent Stem Cell-Derived Neural Constructs for Predicting Neural Toxicity Human embryonic stem cell-derived neural progenitor cells, endothelial cells, mesenchymal stem cells, and microglia/macrophage precursors were combined on chemically defined polyethylene glycol hydrogels and cultured in serum-free medium to model cellular interactions within the developing brain. The precursors self-assembled into 3D neural constructs with diverse neuronal and glial populations, interconnected vascular networks, and ramified microglia. [Proc Natl Acad Sci USA] Abstract | Full Article | Press Release Laser-Based Three-Dimensional Multiscale Micropatterning of Biocompatible Hydrogels for Customized Tissue Engineering Scaffolds The authors present the use of relatively low-energy ultrafast laser pulses to generate 2D and 3D multiscale patterns in soft silk protein hydrogels without exogenous or chemical cross-linkers. Examples illustrating the materials, results, and the performance of the machined geometries in vitro and in vivo are presented to demonstrate the versatility of the approach. [Proc Natl Acad Sci USA] Abstract | Press Release Extracellular Matrix Deposition of Bone Marrow Stroma Enhanced by Macromolecular Crowding Human mesenchymal stem cell (MSC)-derived extracellular matrices (ECM) was produced upon supplementation of standard culture medium with three different macromolecules of various size. Matrix secretion, ECM morphology and composition were compared for matrices obtained from crowded and non-crowded MSC cultures. [Biomaterials] Abstract GLUT10 Deficiency Leads to Oxidative Stress and Non-Canonical αvβ3 Integrin-Mediated TGFβ Signaling Associated with Extracellular Matrix Disarray in Arterial Tortuosity Syndrome Skin Fibroblasts To discern the molecular mechanisms underlying the arterial tortuosity syndrome etiology, investigators performed gene expression profiling and biochemical studies on skin fibroblasts. Transcriptome analyses revealed the dysregulation of several genes involved in TGFβ signaling and extracellular matrix homeostasis as well as the perturbation of specific pathways that control both the cell energy balance and the oxidative stress response. [Hum Mol Genet] Abstract | Full Article Production of Decellularized Porcine Lung Scaffolds for Use in Tissue Engineering A new decellularization technique is described that maintains the global tissue architecture, key matrix components, mechanical composition and cell-seeding potential of lung tissue while effectively removing resident cellular material. [Integr Biol] Abstract Effect of Collagen-Glycosaminoglycan Scaffold Pore Size on Matrix Mineralization and Cellular Behavior in Different Cell Types Scientists aimed to examine the effects of mean pore size on subsequent osteoblast differentiation and matrix mineralization, and to understand the mechanism by which pore size influences behavior of different cell types. [J Biomed Mater Res A] Abstract |