Cell-Free 3D Scaffold with Two-Stage Delivery of miRNA-26a to Regenerate Critical-Sized Bone Defects Researchers showed that a hyperbranched polymer with high microRNA (miRNA)-binding affinity and negligible cytotoxicity can self-assemble into nano-sized polyplexes with a ‘double-shell’ miRNA distribution and high transfection efficiency. These polyplexes are encapsulated in biodegradable microspheres to enable controllable two-stage (polyplexes and miRNA) delivery. [Nat Commun] Full Article | Press Release Application of Marrow Mesenchymal Stem Cell-Derived Extracellular Matrix in Peripheral Nerve Tissue Engineering Based on the fact that bone marrow mesenchymal stem cells (BMSCs), just like Schwann cells, are adopted as support cells within a neural scaffold, scientists used BMSCs as parent cells to generate extracellular matrix for application in peripheral nerve tissue engineering. [J Tissue Eng Regen Med] Abstract Elasticity Modulation of Fibroblast-Derived Matrix for Endothelial Cell Vascular Morphogenesis and Mesenchymal Stem Cell Differentiation Fibroblast-derived matrix was used as cell culture substrate and physically modified to investigate the influence of its biophysical properties changes on human umbilical vein endothelial cells and human mesenchymal stem cells behaviors in vitro. [Tissue Eng Part A] Abstract Decellularization of Porcine Skeletal Muscle Extracellular Matrix for the Formulation of a Matrix Hydrogel: A Preliminary Study Investigators aimed to optimize the decellularization process of porcine skeletal muscle extracellular matrix (ECM) and to formulate a matrix hydrogel scaffold. Five multi-step methods were used to generate acellular ECM from porcine skeletal muscle. [J Cell Mol Med] Full Article Extracellular Matrix Stiffness Controls VEGF Signaling and Processing in Endothelial Cells Scientists hypothesized that extracellular matrix (ECM) stiffness modifies vascular endothelial growth factor A (VEGF)-cell-matrix tethering leading to altered VEGF processing and signaling. The analyzed VEGF binding, internalization, and signaling as a function of substrate stiffness in endothelial cells cultured on fibronectin linked polyacrylamide gels. [J Cell Physiol] Abstract Secretome Profiling of Oral Squamous Cell Carcinoma-Associated Fibroblasts Reveals Organization and Disassembly of Extracellular Matrix and Collagen Metabolic Process Signatures Investigators searched for differences in the secretome of cancer-associated fibroblasts and normal oral fibroblasts using mass spectrometry-based proteomics and biological network analysis. Comparison of the secretome profiles revealed that upregulated proteins involved mainly in extracellular matrix organization and disassembly and collagen metabolism. [Tumor Biol] Abstract Advanced Biomaterial Strategies to Transplant Preformed Micro-Tissue Engineered Neural Networks into the Brain Investigators advanced micro-tissue engineering techniques to generate micro-tissue engineered neural networks consisting of discrete populations of mature primary cerebral cortical neurons spanned by long axonal fascicles encased in miniature hydrogel micro-columns. [J Neural Eng] Full Article | Press Release Collagen Membranes Adsorb the Transforming Growth Factor-β Receptor I Kinase-dependent Activity of Enamel Matrix Derivative Scientists studied the ability of two collagen membranes and a collagen matrix to adsorb the activity intrinsic to enamel matrix derivative that provokes transforming growth factor-beta signaling in oral fibroblasts. [J Periodontol] Abstract Natural Extracellular Matrix Scaffolds Recycled from Human Salivary Digests: A Morphometric Study Researchers tested the hypothesis of preserving the ‘residual connective tissue’ remaining after mechanical and enzymatic release of cells from human submandibular gland biopsies (natural extracellular matrix scaffolds) to be used as recycled natural scaffolds. [Oral Dis] Abstract |