Biologic-Free Mechanically Induced Muscle Regeneration Because muscle is known to respond to mechanical loading, researchers investigated instead whether a material system capable of massage-like compressions could promote regeneration. [Proc Natl Acad Sci USA] Abstract | Press Release Cell–Cell Communication Enhances the Capacity of Cell Ensembles to Sense Shallow Gradients during Morphogenesis Scientists showed experimentally that, whereas individual mammary epithelial cells are incapable of sensing extremely weak gradients of a growth factor, cellular collectives in organotypic cultures exhibit reliable, gradient-driven, directional growth. [Proc Natl Acad Sci USA] Abstract | Press Release Induction of Mesenchymal Stem Cell Chondrogenic Differentiation and Functional Cartilage Microtissue Formation for In Vivo Cartilage Regeneration by Cartilage Extracellular Matrix-Derived Particles Scientists propose a method of preparing a novel cell carrier derived from natural cartilage extracellular matrix (ECM), designated cartilage ECM-derived particles (CEDPs). Under microgravity culture conditions in a rotary cell culture system, bone marrow stromal cells can proliferate rapidly on the surface of CEDPs with high viability. [Acta Biomater] Abstract Hypoxic Stellate Cells of Pancreatic Cancer Stroma Regulate Extracellular Matrix Fiber Organization and Cancer Cell Motility Investigators showed that microenvironment remodeling by hypoxic pancreatic stellate cells promotes cancer cell motility through alteration of extracellular matrix fiber architecture. [Cancer Lett] Abstract Decellularized Liver Extracellular Matrix as Promising Tools for Transplantable Bioengineered Liver Promotes Hepatic Lineage Commitments of Induced Pluripotent Stem Cells Supplementation of extracellular matrix from porcine decellularized liver containing liver-derived growth factors stimulated the albumin expression of porcine induced pluripotent stem cell-derived hepatocytes (iPSC-Heps) during differentiation procedures. The iPSC-Heps were reseeded into decellularized liver scaffolds and the recellularized liver was cultured using a continuous perfusion system. [Tissue Eng Part A] Abstract Monocytes Increase Human Cardiac Myofibroblast-Mediated Extracellular Matrix Remodeling through TGF-β1 Researchers assessed the influence of peripheral blood monocytes on human cardiac myofibroblast activity in a 3D extracellular matrix microenvironment. When cells were cultured under direct contact conditions, multiplex analysis of the co-culture media revealed an increase in the paracrine factors TGF-β1 and matrix metalloproteinase 9 compared to baseline. [Am J Physiol Heart Circ Physiol] Abstract Immunomodulatory Effects of Amniotic Membrane Matrix Incorporated into Collagen Scaffolds Researchers showed that scaffolds containing amniotic membrane matrix have significantly increased mechanical properties and that tendon cells within these scaffolds have increased metabolic activity even when the media is supplemented with the pro-inflammatory cytokine interleukin-1 beta. [J Biomed Mater Res A] Abstract MAGP2 Controls Notch via Interactions with RGD Binding Integrins: Identification of a Novel ECM – Integrin – Notch Signaling Axis The authors report that MAGP2 (microfibril associated glycoprotein 2) cell type specific control of Notch is independent of individual Notch receptor-ligand combinations but dependent on interaction with RGD binding integrins. [Exp Cell Res] Abstract Fibronectin Deposition Participates in Extracellular Matrix Assembly and Vascular Morphogenesis Scientists examined the role of polymerized fibronectin during vascular tubulogenesis using a three-dimensional (3D) cell-derived de-cellularized matrix. A fibronectin-rich 3D de-cellularized extracellular matrix was used as a scaffold to study vascular morphogenesis of endothelial cells. [PLoS One] Full Article |