Flow Induced Crystallization of Collagen: A Potentially Critical Mechanism in Early Tissue Formation The authors present a simple fiber drawing experiment, which demonstrates that slightly concentrated type I collagen monomers can be “flow-crystallized” to form highly-oriented, continuous, hierarchical fibers at cell-achievable strain rates and physiologically relevant concentrations. [ACS Nano] Abstract In Situ Regeneration of Bioactive Coatings Enabled by an Evolved Staphylococcus aureus Sortase A Researchers demonstrated the capacity to repeatedly regenerate a covalently immobilized monomolecular thin film of bioactive molecules through a two-step stripping and recharging cycle. Laboratory evolved S. aureus sortase A transpeptidation facilitated surface re-engineering of medical devices in situ after in vivo implantation through removal and restoration film constituents. [Nat Commun] Full Article | Press Release Morphogenesis of 3D Vascular Networks Is Regulated by Tensile Forces Scientists assessed the effect of uniaxial cell-induced and externally applied tensile forces on the morphology of vascular networks formed within fibroblast and endothelial cell-embedded 3D polymeric constructs. [Proc Natl Acad Sci USA] Abstract | Press Release Matrix Metalloproteinase-10 Regulates Stemness of Ovarian Cancer Stem-Like Cells by Activation of Canonical Wnt Signaling and Can Be a Target of Chemotherapy-Resistant Ovarian Cancer Scientists identified matrix metalloproteinase-10 (MMP10) is expressed in cancer stem-like cells/cancer-initiating cells of epithelial ovarian cancer. An immunohistochemical study revealed that a high expression level of MMP10 is a marker for poor prognosis and platinum resistance in multivariate analysis. [Oncotarget] Full Article Scaffold-Free Scleraxis-Programmed Tendon Progenitors Aid in Significantly Enhanced Repair of Full-Size Achilles Tendon Rupture Investigators compared, by in-depth histomorphometric evaluation, the regenerative potential of uncommitted human mesenchymal stem cells and Scleraxis-programmed tendon progenitors in the healing of a full-size of rat Achilles tendon defect. [Nanomedicine] Abstract Bioactive Anti-Thrombotic Modification of Decellularized Matrix for Vascular Applications Small intestinal submucosa was modified with the endothelial cell membrane protein thrombomodulin and the glycosaminoglycan heparin to facilitate protein C activation and anticoagulant activity, respectively. [Adv Healthc Mater] Abstract Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering Scientists investigated the effect of the incorporation of collagen type I in 3D-bioprinted polysaccharide-based hydrogels to the modulation of cell morphology, osteogenic remodeling potential, and mineralization. [Adv Healthc Mater] Abstract Biomimetic Polymers for Cardiac Tissue Engineering Researchers developed an injectable biomimetic reverse thermal gel (RTG) specifically engineered to support long-term cardiomyocyte (CM) survival. This RTG biopolymer provided a solution-based delivery vehicle of CMs, which transitioned to a gel-based matrix shortly after reaching body temperature. [Biomacromolecules] Full Article | Graphical Abstract Biglycan Potentially Regulates Angiogenesis during Fracture Repair by Altering Expression and Function of Endostatin Scientists showed that endothelial cells treated with endostatin had significantly decreased vessel length and vessel branches compared to untreated cells, while cells treated with endostatin and biglycan at a 1:1 M ratio had vessel length and vessel branches comparable to untreated cells. [Matrix Biol] Abstract CD154 Induces Matrix Metalloproteinase-9 Secretion in Human Podocytes Investigators studied the impact of the CD154/CD40 pathway on matrix metalloproteinase-9 expression by cultured human podocytes. The role of CD40/CD154 in matrix remodeling was evaluated upon exposure of podocytes to recombinant human CD154 or activated platelet supernatants from healthy human subjects. [J Cell Biochem] Abstract |