Hyaluronan Hydrogels for a Biomimetic Spongiosa Layer of Tissue Engineered Heart Valve Scaffolds Hyaluronan hydrogels were tuned to perform the mechanical functions of the spongiosa layer, provide a biomimetic scaffold in which valve cells were encapsulated in 3D for tissue engineering applications, and to gain insight into how valve cells maintain hyaluronan homeostasis within heart valves. [Biomacromolecules] Abstract | Graphical Abstract Self-Assembled Biodegradable Nanoparticles and Polysaccharides as Biomimetic ECM Nanostructures for the Synergistic Effect of RGD and BMP-2 on Bone Formation Biomimetic extracellular matrix (ECM) nanostructures are constructed through layer-by-layer self-assembling positively charged chitosan (Chi), negatively charged oxidized sodium alginate (OAlg), and positively charged bovine serum albumin-based nanoparticles. The results of bone marrow stem cells culture demonstrated that the penta-peptide glycine-arginine-glycine-aspartate-serine grafted Chi/OAlg films favor cell adhesion and proliferation. [Sci Rep] Full Article LIMK Regulates Tumor-Cell Invasion and Matrix Degradation through Tyrosine Phosphorylation of MT1-MMP Investigators identified membrane type 1-matrix metalloproteinase (MT1-MMP) as a novel interacting partner of dual-specificity LIM Kinase-1 and -2 (LIMK1/2), and provide evidence for phosphorylation of tyrosine Y573 in the cytoplasmic domain of MT1-MMP by LIMK. [Sci Rep] Full Article Regulators of Metastasis Modulate the Migratory Response to Cell Contact under Spatial Confinement Scientists showed that metastatic cells overcome spatial constraints to slide effectively on narrow fiber-like dimensions, whereas nontransformed MCF–10A mammary epithelial cells require much wider micropatterns to achieve moderate levels of sliding. Downregulating the cell-cell adhesion protein, E-cadherin, enables MCF-10A cells to slide on narrower micropatterns; meanwhile, introducing exogenous E-cadherin in metastatic MDA-MB–231 cells increases the micropattern dimension at which they slide. [Biophys J] Full Article | Press Release | Video ILK–PI3K/AKT Pathway Participates in Cutaneous Wound Contraction by Regulating Fibroblast Migration and Differentiation to Myofibroblast The authors investigated the role of integrin-linked kinase (ILK) in cutaneous wound contraction. They found that ILK was involved in cutaneous wound healing in rats, and ILK and PI3K/AKT inhibitors inhibited wound contraction and re-epithelialization, consequently delaying wound healing in vivo. [Lab Invest] Abstract Stiffness-Controlled Three-Dimensional Collagen Scaffolds for Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells into Cardiac Progenitor Cells Investigators developed stiffness-controlled collagen scaffolds to allow proliferation and differentiation of mesenchymal stem cell into cardiac progenitor cells. [J Biomed Mater Res A] Abstract RNA Interference-Mediated Silencing of Speckle-Type POZ Protein Promotes Apoptosis of Renal Cell Cancer Cells Scientists investigated the effects of silencing the speckle-type POZ protein gene on renal cell cancer cells and to explore its possible mechanism. The relevant signaling pathways were the integrin-mediated cell surface interactions pathway and extracellular matrix organization signal pathway. [Onco Targets Ther] Full Article High Glucose-Induced Matrilin-2 Expression in Mouse Mesangial Cells Was Mediated by Transforming Growth Factor Beta 1 (TGF-β1) Researchers evaluated the effect of high glucose on the expression of extracellular matrix protein matrilin-2 and the mechanism underlying this effect by using a mouse mesangial cell line. They demonstrated that matrilin-2 expression was markedly induced by high glucose and TGF-β1. [Biochem Biophys Res Commun] Abstract Ex Vivo Expansion of Circulating CD34+ Cells Enhances the Regenerative Effect on Rat Liver Cirrhosis The authors investigated the efficacy of human ex vivo-expanded CD34+ cells for treatment of cirrhotic rat liver. Expanded CD34+ cells were also increasingly positive for cell surface markers of VE-cadherin, VEGF receptor-2, and Tie-2. The expression of proangiogenic growth factors and adhesion molecules in expanded CD34+ cells increased compared with nonexpanded CD34+ cells. [Mol Ther Methods Clin Dev] Full Article |