Strain Stiffening of Fibrillar Collagen during Individual and Collective Cell Migration Identified by AFM Nanoindentation To detect strain stiffening at the leading edge of normal and tumor cells moving across fibrillar type I collagen, researchers combined AFM nanoindentation and differential field probing with confocal reflection microscopy. [ACS Appl Mater Interfaces] Abstract | Graphical Abstract Surface-Driven Collagen Self-Assembly Affects Early Osteogenic Stem Cell Signaling The authors report how extracellular matrix ligand self-assembly on biomaterial surfaces and the resulting nanoscale architecture can drive stem cell behavior. Cellular and molecular investigations with human bone marrow stromal cells revealed higher osteogenic differentiation and upregulation of focal adhesion-related components on smooth collagen layer coated substrates. [Adv Healthc Mater] Abstract Matrix Stiffness Promotes Cartilage Endplate Chondrocyte Calcification in Disc Degeneration via miR‑20a Targeting ANKH Expression Researchers found that matrix stiffness of cartilage endplate (CEP) was positively correlated with the degree of intervertebral disc degeneration, and stiff matrix, which mimicked the severe degeneration of CEP, promoted inorganic phosphate-induced calcification in CEP chondrocytes. [Sci Rep] Full Article Ginsenoside Rg1 Promotes Osteogenic Differentiation of rBMSCs and Healing of Rat Tibial Fractures through Regulation of GR-Dependent BMP-2/SMAD Signaling Scientists investigated the effect of Rg1 on osteogenic differentiation of cultured bone marrow mesenchymal stem cells (BMSCs) and related mechanisms and on the fracture healing in a fracture model. [Sci Rep] Full Article Microstructured Human Fibroblast-Derived Extracellular Matrix Scaffold for Vascular Media Fabrication Investigators developed an anisotropic fibroblastic extracellular matrix sheet that could replicate the physiological architecture of blood vessels after being assembled into a small diameter vascular conduit. [J Tissue Eng Regen Med] Abstract Acellular Hydroxyapatite-Collagen Scaffolds Support Angiogenesis and Osteogenic Gene Expression in an Ectopic Murine Model: Effects of Hydroxyapatite Volume Fraction Researchers investigated the effect of hydroxyapatite (HA) volume fraction on in vivo angiogenesis and osteogenesis in acellular collagen scaffolds containing 0, 20, and 40 vol % HA after subcutaneous ectopic implantation for up to 12 weeks in mice. [J Biomed Mater Res A] Abstract Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-κB Pathway CD40L suppressed prolyl-4-hydroxylase α1 expression in human aortic smooth muscle cells in a dose- and time-dependent manner, with insignificant changes in MMP2 expression and negative enzymatic activity of MMP9. [PLoS One] Full Article Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels Researchers systematically tested variables associated with photocrosslinking hydrogels for their effects on encapsulated cells in an extrusion 3D printable mixture of methacrylated gelatin/poly-ethylene glycol diacrylate/alginate. The fabrication conditions that produced desired hydrogel mechanical properties were compared against those that optimize aortic valve or mesenchymal stem cell viability. [Ann Biomed Eng] Abstract Non-Canonical Role of Matrix Metalloprotease (MMP) in Activation and Migration of Hepatic Stellate Cells (HSCs) Investigators designed a study to identify the non-canonical role of MMPs in HSCs activation and migration. They speculated that macrophage MMP-8, -10 & -13 might play a non-canonical role in HSCs activation. [Life Sci] Abstract |