Extracellular Matrix News Volume 9.43 | Nov 1 2018

TOP STORY

Biologically Relevant Laminin as Chemically Defined and Fully Human Platform for Human Epidermal Keratinocyte Culture

Scientists demonstrated that their laminin system was comparable to the 3T3-J2 co-culture system in terms of basal markers’ profile, colony-forming efficiency and the ability to form normal stratified epidermal structure in both in vitro and in vivo models. [Nat Commun] Full Article | Press Release

(Ranked by impact factor of the journal)

Collagen Prolyl 4-Hydroxylase 1 Is Essential for HIF-1α Stabilization and TNBC Chemoresistance

Researchers showed that collagen prolyl 4-hydroxylase alpha 1 subunit (P4HA1) protein expression was induced in triple-negative breast cancer (TNBC) and HER2 positive breast cancer. By modulating alpha ketoglutarate and succinate levels P4HA1 expression reduced proline hydroxylation on hypoxia-inducible factor 1α (HIF-1α), enhancing its stability in cancer cells. [Nat Commun] Full Article

IGFBP2 Promotes Vasculogenic Mimicry Formation via Regulating CD144 and MMP2 Expression in Glioma

Enforced expression of IGFBP2 increased network formation of glioma cells in vitro by activating CD144 and matrix metalloproteinase 2 (MMP2). U251 cells with stable knockdown of IGFBP2 led to decreased vasculogenic mimicry formation and tumor progression in an orthotopic mouse model. [Oncogene] Abstract

Hypoxia-Driven Paracrine Osteopontin/Integrin αvβ3 Signaling Promotes Pancreatic Cancer Cell Epithelial-Mesenchymal Transition and Cancer Stem Cell-Like Properties by Modulating FOXM1

Osteopontin from activated pancreatic stellate cells interacted with the transmembrane receptor integrin αvβ3 on pancreatic cancer cells (PCCs) to upregulate FOXM1 expression and induce the malignant phenotypes of PCCs. [Mol Oncol] Abstract | Full Article

Combustion-Derived Particles Inhibit In Vitro Human Lung Fibroblast-Mediated Matrix Remodeling

In vitro dynamic 3D analysis of collagen matrices showed that matrix displacement fields induced by human lung fibroblasts were disturbed when exposed to carbonaceous particles, resulting in inhibition of matrix remodeling. [J Nanobiotechnology] Full Article

The Plant Alkaloid Conophylline Inhibits Matrix Formation of Fibroblasts

Scientists investigated the effects of conophylline on human foreskin fibroblasts, especially focusing on ECM proteins. They revealed that conophylline substantially inhibited incorporation of versican and collagens into the ECM in cells treated with transforming growth factor β (TGF-β), which promoted collagen synthesis, but not in cells not treated with TGF-β. [J Biol Chem] Abstract | Full Article

Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix

Investigators demonstrated a novel approach to generate thyroid grafts using decellularized rat thyroid matrix. The decellularization process retained the intricate 3D microarchitecture with a perfusable vascular network and native ECM components, allowing efficient reseeding of the thyroid matrix with the FRTL-5 rat thyroid cell line generating 3D follicular structures in vitro. [Thyroid] Abstract

Bevacizumab or Fibronectin Gene Editing Inhibits the Osteoclastogenic Effects of Fibroblasts Derived from Human Radicular Cysts

The authors investigated the effects of extra domain A⁺ fibronectin (FN) on bone destruction in human radicular cysts and explored the possibility of editing the FN gene or blocking the related paracrine signaling pathway to inhibit the osteoclastogenesis. [Acta Pharmacol Sin] Abstract

Loss of TP53I11 Enhances the Extracellular Matrix-Independent Survival by Promoting Activation of AMPK

Investigators found that loss of TP53I11 enhanced, and overexpression of TP53I11 suppressed the ECM-independent survival, epithelial-mesenchymal transition, and migration in MCF10A cells. They found that TP53I11 regulated the ECM-independent survival by a TP53-independent way. As a metabolic sensor, AMPK promoted anoikis resistance by inhibiting the AKT/m-TOR/p70S6K signaling pathway. [IUBMB Life] Abstract

Biocompatibility of Injectable Hydrogel from Decellularized Human Adipose Tissue In Vitro and In Vivo

Extracted human decellularized adipose tissue (hDAT) was fabricated into an injectable hydrogel, which could be self-assembled to form a gel under certain conditions. The hDAT hydrogel was nontoxic to human adipose-derived stem cells and could spontaneously induce adipogenic differentiation in vitro. [J Biomed Mater Res B Appl Biomater] Abstract

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Fibroblast Growth Factor Receptors as Treatment Targets in Clinical Oncology

The author describes the clinical experience with FGFR inhibitors to date, and highlights key aspects that might lead to improved response rates and/or the avoidance of acquired resistance, including the selection of patients who are most likely to benefit from treatment, and the use of FGFR inhibitors in combination regimens with other agents. [Nat Rev Clin Oncol] Abstract

Tumor Microenvironment: Interactions and Therapy

Investigators provide knowledge about the tumor microenvironment architecture, multiple signaling, and cross communications between cells within this milieu, and its targeting for immunotherapy of cancer. [J Cell Physiol] Abstract

Visit our reviews page to see a complete list of reviews in the extracellular matrix research field.

Cook Regentec Celebrates Opening of Indianapolis Facility, Announces Collaboration with Indiana University School of Medicine

Cook Regentec, Cook Group’s first company in Indianapolis, cut the ribbon on its new facility on Indiana Avenue and announced a collaboration with Indiana University School of Medicine aimed at developing tools and technologies that advance solutions in the emerging areas of immunotherapy, precision medicine, drug delivery, regenerative medicine and bioprinting. [The Trustees of Indiana University] Press Release

Humacyte Commences Phase II Vascular Trauma Trial

Humacyte announced the initiation of a U.S. Phase II vascular trauma clinical trial of HUMACYL^®, its investigational human acellular vessel, for vascular replacement or reconstruction in patients with life- or limb-threatening vascular trauma. [Humacyte, Inc.] Press Release

BioSpecifics Technologies Corp. Announces Positive Topline Results from Phase I Study of CCH for the Treatment of Uterine Fibroids

BioSpecifics Technologies Corp. announced positive topline data from its Phase I trial of Collagenase Clostridium Histolyticum (CCH) for the treatment of uterine fibroids. [BioSpecifics Technologies Corp] Press Release

Avance^® Nerve Graft Receives Regenerative Medicine Advanced Therapy (RMAT) Designation

AxoGen, Inc. reported that the FDA has granted the RMAT designation to Avance^® Nerve Graft. [AxoGen, Inc.] Press Release

NSF Suspends Program Allowing Graduate Fellows to Study Abroad

The U.S. National Science Foundation (NSF) has halted a program that each year allows hundreds of the nation’s best graduate students to work with experts in another country. And the agency isn’t saying why-or whether the program will resume. [ScienceInsider] Editorial

What Will a New California Law Mean for Biotech? Hundreds More Women in the Boardroom

In the next few years, publicly traded life sciences companies headquartered in California could collectively need to add hundreds of women to their boards in order to comply with a new state gender-parity law. That’s going to mean finding women to fill 126 board seats in the sector by the end of 2019. [STAT News] Editorial

NEW Cell Symposia: Engineering Organoids and Organs
August 25-27, 2019
San Diego, California, United States

Visit our events page to see a complete list of events in the community.

NEW Associate Professor/ Professor – Regenerative Medicine (University of Toronto)

Research Fellow – Immunology (King’s College London)

Faculty Position – Biochemistry & Molecular Biology (Mayo Clinic)

Postdoctoral Positions – Cellular Immunology and Lattice Light-Sheet Imaging (University of New South Wales)

Postdoctoral Fellow – Bone Regeneration (University of Michigan)

Tenure Track Assistant Professor – Biochemistry and Life Sciences (University of Delaware)

Group Leader – Organoid Modelling of Disease (European Molecular Biology Laboratory)

Tenure-Track Faculty Position – Cell Signaling & ECM (University of Rochester)

Postdoctoral Position – Cutaneous Wound Repair (University of Pennsylvania)

Postdoctoral Scholar – Cellular Mechanobiology (Washington University in St. Louis)

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