Extracellular Matrix News 9.46 November 22, 2018 | |
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TOP STORYInvestigators demonstrated the fabrication of genetically tunable ECM-derived materials, by utilizing WT and TSP-2 KO decellularized skins to prepare hydrogels. The resulting materials exhibited distinct mechanical properties characterized by rheology, and differential concentrations of collagens when characterized by quantitative proteomics. [ACS Appl Mater Interfaces] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Tissue constructs contained a confluent human endothelium grown atop a tri-dimensional human extracellular matrix substrate, employed human age-specific monocytes and autologous non heat-treated plasma, and avoided the use of xenogenic materials and exogenous cytokines. [Front Immunol] Full Article | Press Release Researchers designed a hydrostatic pressure bioreactor system that was capable of stimulating cartilage cell cultures with elevated reactive oxygen species (ROS) levels. Increased ROS levels in the media did not only lead to an inhibition of glycosaminoglycans and collagen II formation but also to a reduction of already formed glycosaminoglycans and collagen II in chondrogenic mesenchymal stem cell pellet cultures. [Sci Rep] Full Article Scientists investigated changes in energy metabolism due to collagen density in five breast cell lines by measuring the fluorescence lifetime of NADH. They found that only triple-negative breast cancer cells, MDA-MB231 and MDA-MB468 cells, had an increased population of bound NADH, indicating an oxidative phosphorylation signature, as collagen density decreased. [Sci Rep] Full Article Researchers investigated the relationship between transforming growth factor-β (TGF-β) stimulation and collagen expression and explored the role of connective tissue growth factor (CTGF) during the remodeling process using human chronic rhinosinusitis without nasal polyp (CRSsNP) nasal mucosa tissues and mucosa-derived fibroblasts as main materials. [J Cell Physiol] Abstract The authors found that Arid2-IR was increased in high-fat diet and streptozotocin-induced type 2 diabetes mice and in mouse mesangial cells cultured with high glucose to mimic diabetes. Knockdown of Arid2-IR in mouse mesangial cells reduced the high expression of Col1a1 and α-SMA induced by high glucose. [Am J Physiol Cell Physiol] Abstract The effects of serum amyloid A1 (SAA1) on the abundance of collagenases and lysyl oxidase, the enzyme that cross-links collagens, were investigated in the culture of primary human amnion fibroblasts and tissue explants with an aim to examine the involvement of SAA1 in ECM remodeling in the amnion. [Am J Reprod Immunol] Abstract Vildagliptin Reduced Extracellular Matrix Degradation in Human Primary Chondrocytes Vildagliptin reduced degradation of the articular ECM by downregulating IL-1β-induced expression of matrix metalloproteinases (MMP)-3, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5. [Eur J Pharmacol] Abstract In vitro analysis of the ECM sheets showed efficient decellularisation; absence of immunostimulatory components; and the presence of a wide number of angiogenic and bioactive factors, including collagen, elastin, and proteoglycans. [Int Wound J] Abstract Both tumor necrosis factor α (TNFα)-induced matrix metalloproteinase 9 (MMP9) mRNA expression and activity were significantly inhibited by pretreatment with medroxyprogesterone acetate (MPA); however, only the inhibition of TNFα-induced MMP9 activity was partially reversed with progesterone receptor membrane component 1 siRNA. However, glucocorticoid receptor siRNA reversed both the inhibition of TNFα-induced MMP9 mRNA expression and activity by MPA. [Reprod Sci] Abstract There was a significant reduction in miR-202-3p expression in the severe degenerative disc compared with mild degenerative disc. Down-regulation of miR-202-3p expression by interleukin-1β was correlated with up-regulation of matrix metalloproteinase 1 expression in human nucleus pulposus cells. [Gene] Abstract Subscribe to one of our other 19 science newsletters such as Endothelial Cell News & Dermal Cell News. | |
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REVIEWSRoles of Integrins and Extracellular Matrix in Stroke The authors describe the affect that various integrins and extracellular matrix components have in embryonic brain development, and how this changes in both maturity and in the post-stroke environment, with particular focus on how these changes affect blood-brain barrier integrity and their potential as diagnostic and therapeutic targets for ischemic stroke. [Am J Physiol Cell Physiol] Abstract Visit our reviews page to see a complete list of reviews in the extracellular matrix research field. | |
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INDUSTRY NEWSDFG to Fund 15 New Research Training Groups The Deutsche Forschungsgemeinschaft (DFG) is establishing 15 new Research Training Groups (RTGs) to further support early career researchers. This was decided by the responsible Grants Committee during its autumn session in Bonn. The new RTGs will receive a total of approximately €71 million for an initial period of four and a half years, starting from April 2019. [DFG] Press Release Permanent J Code Issued for Avedro’s Photrexa® Drug Formulations Avedro, Inc. announced that the Centers for Medicare and Medicaid Services issued a product-specific J code, J2787, for Photrexa® Viscous and Photrexa®. These Photrexa formulations are the only drugs approved by the FDA for use in corneal collagen cross-linking for the treatment of progressive keratoconus and corneal ectasia following refractive surgery. [Avedro, Inc.] Press Release | |
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POLICY NEWSFinal Gender Discrimination Case at Salk Institute Ends in Settlement The prestigious Salk Institute for Biological Studies in La Jolla, California, has settled the final of three high-profile gender-discrimination lawsuits filed last year. The latest agreement was announced on 21 November. [Nature News] Editorial AI Peer Reviewers Unleashed to Ease Publishing Grind A suite of automated tools is now available to assist with peer review but humans are still in the driver’s seat. A handful of academic publishers are piloting AI tools to do anything from selecting reviewers to checking statistics and summarizing a paper’s findings. [Nature News] Editorial To Catch Misconduct, Journals Are Hiring Research Integrity Czars Scientific journals’ creation of dedicated positions for rooting out misconduct before publication comes amid growing awareness of such issues, and stems from a recognition that spot-checking and other ad hoc arrangements were insufficient. [STAT News] Editorial In Reversal, NSF Lifts Proposal Limits on Biologists In a reversal, the National Science Foundation (NSF) will no longer restrict researchers to only one proposal submission per year to the biology directorate’s three core tracks in which they are listed as a principal investigator (PI) or co-PI. [ScienceInsider] Editorial
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EVENTSNEW EMBO-EMBL: Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Position – Regenerative Medicine, Stem Cells & Bioengineering (Igenomix Foundation) Tenured Track Faculty Position – Biomedical Engineering (Johns Hopkins University) Postdoctoral Research Fellow – Pathogenesis of Sepsis and Lung Inflammation (Boston University) Postdoctoral Fellowship Program – Aging (Niigata University) Associate Professor/ Professor – Regenerative Medicine (University of Toronto) Research Fellow – Immunology (King’s College London) Faculty Position – Biochemistry & Molecular Biology (Mayo Clinic) Postdoctoral Fellow – Bone Regeneration (University of Michigan) Postdoctoral Position – Cutaneous Wound Repair (University of Pennsylvania) Postdoctoral Scholar – Cellular Mechanobiology (Washington University in St. Louis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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