Hematopoiesis News Volume 12.00 | Jan 5 2021

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    2020-05-01 | HN 12.00


    Hematopoiesis News by STEMCELL Technologies
    Vol. 12.00 – 5 January, 2021
    TOP STORY

    ZNF143
    Mediates CTCF-Bound Promoter–Enhancer Loops Required for Murine Hematopoietic Stem and Progenitor Cell Function

    Zinc finger protein 143 (ZNF143)-CCCTC binding factor (CTCF)-bound promoter–enhancer loops regulated gene expression patterns essential for maintenance of murine hematopoietic stem and progenitor cell integrity.
    [Nature Communications]

    Full Article

    Dr. Jackie Damen discusses why the CFU assay should be a vital part of your cell therapy workflow in this on-demand webinar.
    PUBLICATIONSRanked by the impact factor of the journal

    Targeting
    USP47 Overcomes Tyrosine Kinase Inhibitor Resistance and Eradicates Leukemia Stem/Progenitor Cells in Chronic Myelogenous Leukemia

    The knockout of Usp47 significantly inhibited BCR-ABL and BCR-ABLT315I-induced CML in mice with the reduction of LinSca1+c-Kit+ chronic myelogenous leukemia stem/progenitor cells.
    [Nature Communications]

    Full Article

    OGT
    Regulates Hematopoietic Stem Cell Maintenance via PINK1-Dependent Mitophagy

    Using O-linked N-acetylglucosamine transferase (Ogt) conditional knockout mice, researchers showed that OGT was essential for hematopoietic stem and progenitor cells (HSPCs). Ogt was highly expressed in HSPCs, and its disruption induced rapid loss of HSPCs with increased reactive oxygen species and apoptosis.
    [Cell Reports]

    Full ArticleGraphical Abstract

    USP15
    Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells

    The authors systematically inactivated deubiquitinating enzymes (DUBs) in mouse hematopoietic progenitors using in vivo small hairpin RNA (shRNA) screens. They found that multiple DUBs may be individually required for hematopoiesis and identify ubiquitin-specific protease 15 (USP15) as essential for hematopoietic stem cell maintenance in vitro and in transplantations and Usp15 knockout (KO) mice in vivo.
    [Cell Reports]

    Full ArticleGraphical Abstract

    The Vascular Niche Controls Drosophila
    Hematopoiesis via Fibroblast Growth Factor Signaling

    Investigators report that the vascular system acts as a second niche to control lymph gland homeostasis. The FGF ligand Branchless produced by vascular cells activated the FGF pathway in hematopoietic progenitors.
    [eLife]

    Full Article

    Chronic
    Lymphocytic Leukemia B Cell Derived TNFα Impairs Bone Marrow Myelopoiesis

    The authors showed that co-culture of chronic lymphocytic leukemia B cells with purified normal human hematopoietic stem and progenitor cells (HSPCs) directly altered protein levels of the myeloid and erythroid cell fate determinants PU.1 and GATA-2 at the single cell level within transitional HSPC subsets, mimicking ex vivo expression patterns.
    [iScience]

    AbstractFull Article
    Graphical Abstract

    Combined
    Lentiviral- and RNA-Mediated CRISPR/Cas9 Delivery for Efficient and Traceable Gene Editing in Human Hematopoietic Stem and Progenitor Cells

    Researchers developed a combinatorial system using stable lentiviral delivery of single guide RNA (sgRNA) followed by transient transfection of Cas9 mRNA by electroporation in human cord blood-derived CD34+ hematopoietic stem and progenitor cells.
    [Scientific Reports]

    Full Article

    Fetal Sheep Supports the Development of Hematopoietic Cells In Vivo
    from Human iPSCs

    The authors hypothesized that the hematopoietic microenvironment, existing in fetal liver but lacking in vitro, turns induced pluripotent stem cell-derived hemogenic endothelial cells (iPSC-HEs) to hematopoietic stem and progenitor cells (HSPCs). To test this, they transplanted CD45-negative iPSC-HEs into fetal sheep liver, in which HSPCs first grow.
    [Experimental Hematology]

    Abstract

    Different
    Mutant RUNX1 Oncoproteins Program Alternate Hematopoietic Differentiation Trajectories

    Researchers showed that each class of mutant RUNX1 deregulated endogenous RUNX1 function by a different mechanism, leading to specific alterations in developmentally controlled transcription factor binding and chromatin programming.
    [Life Science Alliance]

    Full Article

    CLINICAL RESEARCH

    Effect
    of Donor Type on Volume of Blood Transfusions Required after Allogeneic Hematopoietic Cell Transplantation

    Scientists reviewed blood product use in 729 consecutive allogeneic hematopoietic cell transplantation (allo-HCT) recipients at our center to assess the volume of red blood cells and platelets required after allo-HCT.
    [International Journal of Hematology]

    Abstract


    Explore scientific events this January with the Science Events Calendar.
    REVIEWS

    Studying
    Leukemia Stem Cell Properties and Vulnerabilities with Human iPSCs

    Investigators discuss the lessons that can be learned about the leukemia stem cell (LSC) state, its plasticity, stability and genetic and epigenetic determinants from induced pluripotent stem cell (iPSC) modeling. They also discuss the practical and translational implications of exploiting acute myeloid leukemia (AML)-iPSCs to prospectively isolate large numbers of iLSCs for large-scale experiments, such as screens, and for discovery of new therapeutic targets specific to AML LSCs.
    [Stem Cell Research]

    Full Article

    INDUSTRY AND POLICY NEWS

    AGC Biologics Confirms Cell and Gene Therapy Commercial Expertise as Manufacturer of Orchard Therapeutics’ Newly Approved Libmeldy™

    AGC Biologics is the first manufacturer of Orchard Therapeutics’ Libmeldy™, which was recently approved by the European Commission as a one-time therapy for eligible patients with early-onset Metachromatic Leukodystrophy.
    [AGC Biologics]

    Press Release

    FEATURED EVENT

    ISSCR: Stem Cells and Global Sustainability

    February 3 – 23, 2021
    Virtual


    > See All Events

    JOB OPPORTUNITIES

    Senior
    Investigator – Replication Stress and Chromatin Alterations

    NIH National Cancer Institute – Bethesda, Maryland, United States

    Scientist – Autoimmunity in AML

    Rubius Therapeutics – Cambridge, Massachusetts, United States


    Scientist – Blood Cell Development and Disorders

    St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

    Research Professional – Life Sciences

    Stanford University – Stanford, California, United States

    Research
    Fellow – Stem Cell Biology & Engineering

    New York Blood Center – New York, New York, United States

    > See All Jobs

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