| Vol. 12.00 – 5 January, 2021 |
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| Zinc finger protein 143 (ZNF143)-CCCTC binding factor (CTCF)-bound promoter–enhancer loops regulated gene expression patterns essential for maintenance of murine hematopoietic stem and progenitor cell integrity. [Nature Communications] |
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| PUBLICATIONSRanked by the impact factor of the journal |
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| The knockout of Usp47 significantly inhibited BCR-ABL and BCR-ABLT315I-induced CML in mice with the reduction of Lin−Sca1+c-Kit+ chronic myelogenous leukemia stem/progenitor cells. [Nature Communications] |
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| Using O-linked N-acetylglucosamine transferase (Ogt) conditional knockout mice, researchers showed that OGT was essential for hematopoietic stem and progenitor cells (HSPCs). Ogt was highly expressed in HSPCs, and its disruption induced rapid loss of HSPCs with increased reactive oxygen species and apoptosis. [Cell Reports] |
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| The authors systematically inactivated deubiquitinating enzymes (DUBs) in mouse hematopoietic progenitors using in vivo small hairpin RNA (shRNA) screens. They found that multiple DUBs may be individually required for hematopoiesis and identify ubiquitin-specific protease 15 (USP15) as essential for hematopoietic stem cell maintenance in vitro and in transplantations and Usp15 knockout (KO) mice in vivo. [Cell Reports] |
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| Investigators report that the vascular system acts as a second niche to control lymph gland homeostasis. The FGF ligand Branchless produced by vascular cells activated the FGF pathway in hematopoietic progenitors. [eLife] |
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| The authors showed that co-culture of chronic lymphocytic leukemia B cells with purified normal human hematopoietic stem and progenitor cells (HSPCs) directly altered protein levels of the myeloid and erythroid cell fate determinants PU.1 and GATA-2 at the single cell level within transitional HSPC subsets, mimicking ex vivo expression patterns. [iScience] |
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| Researchers developed a combinatorial system using stable lentiviral delivery of single guide RNA (sgRNA) followed by transient transfection of Cas9 mRNA by electroporation in human cord blood-derived CD34+ hematopoietic stem and progenitor cells. [Scientific Reports] |
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| The authors hypothesized that the hematopoietic microenvironment, existing in fetal liver but lacking in vitro, turns induced pluripotent stem cell-derived hemogenic endothelial cells (iPSC-HEs) to hematopoietic stem and progenitor cells (HSPCs). To test this, they transplanted CD45-negative iPSC-HEs into fetal sheep liver, in which HSPCs first grow. [Experimental Hematology] |
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| Researchers showed that each class of mutant RUNX1 deregulated endogenous RUNX1 function by a different mechanism, leading to specific alterations in developmentally controlled transcription factor binding and chromatin programming. [Life Science Alliance] |
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| Scientists reviewed blood product use in 729 consecutive allogeneic hematopoietic cell transplantation (allo-HCT) recipients at our center to assess the volume of red blood cells and platelets required after allo-HCT. [International Journal of Hematology] |
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| Investigators discuss the lessons that can be learned about the leukemia stem cell (LSC) state, its plasticity, stability and genetic and epigenetic determinants from induced pluripotent stem cell (iPSC) modeling. They also discuss the practical and translational implications of exploiting acute myeloid leukemia (AML)-iPSCs to prospectively isolate large numbers of iLSCs for large-scale experiments, such as screens, and for discovery of new therapeutic targets specific to AML LSCs. [Stem Cell Research] |
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| AGC Biologics is the first manufacturer of Orchard Therapeutics’ Libmeldy™, which was recently approved by the European Commission as a one-time therapy for eligible patients with early-onset Metachromatic Leukodystrophy. [AGC Biologics] |
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| February 3 – 23, 2021 Virtual |
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| NIH National Cancer Institute – Bethesda, Maryland, United States |
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| Rubius Therapeutics – Cambridge, Massachusetts, United States |
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| St. Jude Children’s Research Hospital – Memphis, Tennessee, United States |
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| Stanford University – Stanford, California, United States |
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| New York Blood Center – New York, New York, United States |
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