LABORATORY RESEARCH Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum Scientists used single-cell “mass cytometry” to examine healthy human bone marrow, measuring 34 parameters simultaneously in single cells (binding of 31 antibodies, viability, DNA content, and relative cell size). [Science] Accumulating Mitochondrial DNA Mutations Drive Premature Hematopoietic Aging Phenotypes Distinct from Physiological Stem Cell Aging Using a model carrying a proofreading-defective mitochondrial DNA polymerase, researchers demonstrate hematopoietic defects reminiscent of premature HSC aging, including anemia, lymphopenia, and myeloid lineage skewing. [Cell Stem Cell] Uncoupling of GTP Hydrolysis from eIF6 Release on the Ribosome Causes Shwachman-Diamond Syndrome By isolating late cytoplasmic 60S ribosomal subunits from Sbds-deleted mice, investigators show that Shwachman-Bodian-Diamond syndrome (SBDS) and the GTPase elongation factor-like 1 (EFL1) directly catalyze eukaryotic initiation factor 6 (eIF6) removal in mammalian cells by a mechanism that requires GTP binding and hydrolysis by EFL1 but not phosphorylation of eIF6 Ser 235. [Genes Dev] Crebbp Haploinsufficiency in Mice Alters the Bone Marrow Microenvironment Leading to Loss of Stem Cells and Excessive Myelopoiesis Scientists describe that CREB binding protein (CREBBP) plays an equally pivotal role in microenvironment-mediated regulation of hematopoiesis. [Blood] Cysteine Shotgun–Mass Spectrometry (CS-MS) Reveals Dynamic Sequence of Protein Structure Changes within Mutant and Stressed Cells Cysteine shotgun labeling with fluorophores is analyzed with mass spectrometry of the spectrin–actin membrane skeleton in sheared red blood cell ghosts from normal and diseased mice. [Proc Natl Acad Sci U S A] A Novel Role of the CX3CR1/CX3CL1 System in the Cross-Talk Between Chronic Lymphocytic Leukemia Cells and Tumor Microenvironment Researchers investigated whether the CX3CR1/CX3CL1 axis is involved in the interaction of chronic lymphocytic leukemia with their microenvironment. [Leukemia] Regulation of Erythroid Differentiation by miR-376a and Its Targets Investigators report that miR-376a participates in the regulation of the early stages of human erythropoiesis by targeting cyclin-dependent kinase 2 and Argonaute 2. [Cell Res] CLINICAL RESEARCH Long-Term Use of Acetaminophen, Aspirin, and Other Nonsteroidal Anti-Inflammatory Drugs and Risk of Hematologic Malignancies: Results From the Prospective Vitamins and Lifestyle (VITAL) Study Among previous studies examining the associations of over-the-counter analgesics or nonsteroidal anti-inflammatory drugs (NSAIDs) and incident hematologic malignancies, results were inconsistent for NSAIDs but suggested an increased risk with acetaminophen (paracetamol). Herein, scientists used a large prospective cohort study to examine these associations. [J Clin Oncol] A Phase II Study of Lenalidomide Monotherapy in Patients with Deletion 5q Acute Myeloid Leukemia: SWOG Study S0605 This Phase II study explores the safety and efficacy of single agent lenalidomide in previously untreated older AML patients with chromosome 5q deletion who declined standard chemotherapy. [Blood] HIV-Associated Hodgkin Lymphoma During the First Months on Combination Antiretroviral Therapy Researchers evaluated Hodgkin lymphoma risk by use of combination antiretroviral therapies, especially during the first months of treatment. [Blood] Prophylactic Transfer of BCR-ABL–, PR1-, and WT1-Reactive Donor T Cells After T-Cell–Depleted Allogeneic Hematopoietic Cell Transplantation in Patients with Chronic Myeloid Leukemia Scientists investigated the effects of prophylactic infusion of in vitro-generated donor T cells reactive against peptides derived from chronic myeloid leukemia-associated antigens. [Blood] Prognostic Impact of FLT3-ITD Load in NPM1 Mutated Acute Myeloid Leukemia To clarify whether FLT3-ITD load as expressed as FLT3-ITD/ wildtype (wt) ratio is also relevant in patients with NPM1 mutated AML, investigators assessed the FLT3-ITD mutation status and FLT3-ITD/wt ratio by fragment analysis in 638 NPM1mut AML (339 females; 299 males; 17.8–88.0 years), and analyzed its prognostic relevance in 355 patients. [Leukemia] Allogeneic Hematopoietic SCT for Alpha-Mannosidosis: An Analysis of 17 Patients This retrospective multi institutional analysis describes 17 patients that were diagnosed at a median of 2.5 (1.1–23) years and underwent hematopoietic SCT at a median of 3.6 (1.3–23.1) years. [Bone Marrow Transplant] |