LABORATORY RESEARCH Hematopoietic Stem Cell Subtypes Expand Differentially During Development and Display Distinct Lymphopoietic Programs Adult hematopoietic stem cells (HSCs) with serially transplantable activity comprise two subtypes. Researchers show that both HSC subtypes are present in the fetal liver (at a 1:10 ratio) with the rarer, lymphoid-deficient HSCs immediately gaining an increased representation in the fetal bone marrow, suggesting that the marrow niche plays a key role in regulating their ensuing preferential amplification. [Cell Stem Cell] Abstract Trophoblasts Regulate the Placental Hematopoietic Niche through PDGF-B Signaling Investigators identified PDGF-B signaling in trophoblasts as a key component of the unique placental hematopoietic microenvironment that protects hematopoietic stem/progenitor cells from premature differentiation. [Dev Cell] Abstract | Press Release An Acute Negative Bystander Effect of γ-Irradiated Recipients on Transplanted Hematopoietic Stem Cells in Mice In this study, researchers found that irradiated mouse recipients significantly impaired the long-term repopulating ability of transplanted mouse hematopoietic stem cells shortly (~17 hours) after exposure to irradiated hosts and before the cells began to divide. [Blood] Abstract Chromatin Occupancy Analysis Reveals Genome-Wide GATA Factor Switching during Hematopoiesis Here investigators demonstrate on a genome wide scale that the hematopoietic GATA factors GATA-1 and GATA-2 bind overlapping sets of genes, often at distinct sites, as a means to differentially regulate target gene expression and to regulate the balance between proliferation and differentiation. [Blood] Abstract Early Ontogenic Origin of the Hematopoietic Stem Cell Lineage To de-convolute hematopoietic ontogeny, scientists designed an embryo-rescue system in which the key hematopoietic factor Runx1 is reactivated in Runx1-null conceptuses at specific developmental stages. [Proc Natl Acad Sci USA] Abstract Frequent Loss of RAF Kinase Inhibitor Protein Expression in Acute Myeloid Leukemia Researchers investigated RAF kinase inhibitor protein’s role in acute myeloid leukemia, an aggressive malignancy arising from hematopoietic stem and progenitor cells. [Leukemia] Abstract Interleukin-27 Inhibits the Growth of Pediatric Acute Myeloid Leukemia in NOD/SCID/Il2rg−/− Mice Scientists investigated whether interleukin-27, an immunomodulatory cytokine, functions as an antitumor agent against pediatric acute myeloid leukemia cells. [Clin Cancer Res] Abstract Role of Macrophages in Mobilization of Hematopoietic Progenitor Cells from Bone Marrow After Hemorrhagic Shock In this study, scientists investigated the mechanism of hemorrhagic shock with resuscitation regulation of hematopoietic progenitor cell mobilization from bone marrow. [Shock] Abstract CLINICAL RESEARCH Correlation of CD33 Expression Level with Disease Characteristics and Response to Gemtuzumab Ozogamicin Containing Chemotherapy in Childhood AML CD33 mean fluorescent intensity of leukemic blasts was prospectively quantified in 619 de novo pediatric acute myeloid leukemia patients enrolled on Children’s Oncology Group gemtuzumab ozogamicin-containing clinical trials and correlated with disease characteristics and clinical outcome. [Blood] Abstract Allogeneic Hematopoietic Stem Cell Transplantation Following Reduced-Intensity Conditioning Regimen in Children: A Single Center Experience This single-center retrospective study reported the outcome of 19 children treated with a reduced-intensity conditioning regimen prior to allogeneic stem cell transplantation, for hematologic malignancies (n=17), bone marrow failure (n=1) and neuroblastoma (n=1). [Eur J Haematol] Abstract |