Hematopoiesis News Volume 3.45 | Nov 13 2012

LABORATORY RESEARCH

Ezh1 Is Required for Hematopoietic Stem Cell Maintenance and Prevents Senescence-Like Cell Cycle Arrest
Researchers showed that Ezh1 maintains repopulating hematopoietic stem cells (HSCs) in a slow-cycling, undifferentiated state, protecting them from senescence. Ezh1 ablation induces significant loss of adult HSCs, with concomitant impairment of their self-renewal capacity due to a potent senescence response. [Cell Stem Cell] Abstract | Graphical Abstract

LRF-Mediated Dll4 Repression in Erythroblasts Is Necessary for Hematopoietic Stem Cell Maintenance
Scientists report a novel mechanism that prevents hematopoietic stem cells (HSCs) from undergoing premature lymphoid differentiation in bone marrow. Using a series of in vivo mouse models and functional HSC assays, they showed that leukemia/lymphoma related factor (LRF) is necessary for HSC maintenance by functioning as an erythroid-specific repressor of Delta-like 4 (Dll4) expression. [Blood] Abstract

Inhibition of T Cell Protein Tyrosine Phosphatase Enhances IL-18-Dependent Hematopoietic Stem Cell Expansion
Researchers report that inhibition of T cell protein tyrosine phosphatase, an enzyme involved in the regulation of cytokine signaling, through gene knockout results in a nine-fold increase in the number of hematopoietic progenitors in murine bone marrow. [Stem Cells] Abstract

Tie2⁺ Bone Marrow Endothelial Cells Regulate Hematopoietic Stem Cell Regeneration following Radiation Injury
Investigators utilized genetic models to determine if radioprotection of bone marrow endothelial cells could facilitate hematopoietic regeneration following radiation-induced myelosuppression. [Stem Cells] Abstract

SUMO1-Activating Enzyme Subunit 1 Is Essential for the Survival of Hematopoietic Stem/Progenitor Cells in Zebrafish
Researchers report the isolation and characterization of a zebrafish mutant, tango^hkz5, which shows defects of definitive hematopoiesis. In tango^hkz5 mutants, hematopoietic stem/progenitor cells initiate normally in the aorta-gonad-mesonephros and subsequently colonize the caudal hematopoietic tissue. [Development] Abstract

Resveratrol Ameliorates Ionizing Irradiation-Induced Long-Term Hematopoietic Stem Cell Injury in Mice
Previous studies showed that total body irradiation (TBI) induces long-term bone marrow (BM) suppression in part by induction of hematopoietic stem cell (HSC) senescence through NADPH oxidase 4-derived reactive oxygen species. In the present study, scientists examined if resveratrol, a potent antioxidant and a putative activator of Sirtuin 1, can ameliorate TBI-induced long-term BM injury by inhibiting radiation-induced chronic oxidative stress and senescence in HSCs. [Free Radic Biol Med] Abstract

Nitric Oxide Depletion Alters Hematopoietic Stem Cell Commitment toward Immunogenic Dendritic Cells
Investigators depleted autocrine NO^• production, using N^G-Monomethyl-L-arginine monoacetate and paracrine NO^•, using oxy-hemoglobin as a NO^• scavenger during in vitro differentiation of human peripheral blood hemopoietic stem cells to immunogenic dendritic cells. They monitored the NO^• level, cell proliferation, phenotype and differentiation potential. [Biochim Biophys Acta] Abstract

SOD2 Deficiency in Hematopoietic Cells in Mice Results in Reduced Red Blood Cell Deformability and Increased Heme Degradation
To investigate the potential damage to mature red blood cells (RBCs) resulting from super oxide dismutase-2 (SOD2) deficiency in precursor cells, researchers studied RBCs from mice in which fetal liver stem cells deficient in SOD2 were capable of efficiently rescuing lethally irradiated host animals. [Exp Hematol] Abstract

CLINICAL RESEARCH

Randomized Trial of Myeloablative Conditioning Regimens: Busulfan Plus Cyclophosphamide versus Busulfan Plus Fludarabine
Researchers conducted a phase III randomized clinical trial to compare two myeloablative conditioning regimens for allogeneic hematopoietic cell transplantation in patients with leukemia and myelodysplastic syndrome. [J Clin Oncol] Abstract

Allogeneic Hematopoietic Cell Transplantation for XIAP Deficiency: An International Survey Reveals Poor Outcomes
In order to estimate the success of hematopoietic cell transplantation for patients with XIAP deficiency, investigators conducted an international survey of transplant outcomes. Data was reported for 19 patients. [Blood] Abstract

Prediction of Area under the Cyclosporine Concentration vs Time Curve in Children undergoing Hematopoietic Stem Cell Transplant
This prospective study aimed to validate a previously developed first dose limited sampling strategy (LSS) to predict cyclosporine area under the concentration-versus-time curve (AUC) and to develop and then validate an LSS to predict cyclosporine AUC at steady-state. [Biol Blood Marrow Transplant] Abstract

Tissue Regeneration at Heart of New Institute
The emerging field of tissue regeneration, which harnesses the power and healing potential of stem cells, is an area to which scientists around the world are turning their attention. One of the foremost leaders in this area, Mark Sussman from San Diego State University (SDSU), announced the opening of SDSU’s new Integrated Regenerative Research Institute to help advance this area of research in San Diego. [San Diego State University] Press Release

£20 Million Investment for UK Synthetic Biology Research
In a move that could potentially revolutionize major UK industries and help us to meet serious social and environmental challenges, the Biotechnology and Biological Sciences Research Council has announced an unprecedented £20M worth of synthetic biology projects. [Biotechnology and Biological Sciences Research Council] Press Release

National Institutes of Health (United States)

Food and Drug Administration (United States)
 
Center for Biologics Evaluation and Research (United States)
 
European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

NEW Third Annual Scientific Meeting of the Mercia Stem Cell Alliance
Manchester, United Kingdom
December 3, 2012

Visit our events page to see a complete list of events in the hematopoietic community.

Quality Control Operations Coordinator (STEMCELL Technologies, Inc.)

Product Manager – Hematopoietic (STEMCELL Technologies, Inc.)

Product Quality Scientist (STEMCELL Technologies, Inc.)

Scientist – Endothelial Cell Research (STEMCELL Technologies, Inc.)

Research Technologist – Cell Separation (STEMCELL Technologies, Inc.)

Scientist, Postdoctoral and Research Associate Positions (University of Miami – Sylvester Comprehensive Cancer Center)

Postdoctoral Position – Impact of Inflammation on Hematopoiesis and Cancer (University of Murcia)

Postdoctoral Position – Tumor Stem Cells (Cancer Institute of New Jersey)

Senior Lecturer – Cancer Stem Cells (The European Cancer Stem Cell Research Institute)

Postdoctoral Position – Hematopoietic Stem Cell and Leukemia Research (City of Hope Beckman Research Institute) 

Postdoctoral Position – Human Leukemic Stem Cells (University of Groningen)

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