Hematopoiesis News Volume 4.29 | Jul 30 2013

Staphylococcus aureus Recognition by Hematopoietic Stem and Progenitor Cells via TLR2/MyD88/PGE₂ Stimulates Granulopoiesis in Wounds
Previously, the authors reported that hematopoietic stem and progenitor cells (HSPCs) recruited to S. aureus-infected skin wounds in mice, undergo granulopoiesis, while others have demonstrated their differentiation in vitro after TLR2/MyD88 stimulation. In this study, they examined this pathway in HSPC trafficking and granulopoiesis within S. aureus-infected wounds. [Blood] Abstract

The Combined Influence of Substrate Elasticity and Surface-Grafted Molecules on the Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells
Because little is known about the effects of the physical microenvironment on hematopoietic stem and progenitor cell (HSPC) expansion, the authors investigated the ex vivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. [Biomaterials] Abstract

Myeloid/Microglial Driven Autologous Hematopoietic Stem Cell Gene Therapy Corrects a Neuronopathic Lysosomal Disease
To improve brain expression via monocyte/microglial specificity, lentiviral vectors (LVs) expressing enhanced green fluorescent protein (eGFP) under ubiquitous phosphoglycerate kinase (PGK) or myeloid-specific promoters were compared in transplanted hematopoietic stem cells. LV-CD11b-GFP gave significantly higher monocyte/B-cell eGFP expression than LV-PGK-GFP or LV-CD18-GFP after six months. [Mol Ther]
Full Article | Press Release

No Impact of Lentiviral Transduction on Hematopoietic Stem/Progenitor Cell Telomere Length or Gene Expression in the Rhesus Macaque Model
Scientists utilized two techniques to examine whether lentivirally-transduced hematopoietic stem and progenitor cells from eight rhesus macaques transplanted 1-13.5 years previously are perturbed at a population level, comparing telomere length as a measure of replicative history and gene expression profile of vector positive versus vector negative cells. [Mol Ther] Abstract

Isolation, Characterization and Transplantation of Bone Marrow Derived Cell Component with Hematopoietic Stem Cell Niche Properties
Investigators isolated cell complexes based on size fractionation from mouse bone marrow (BM), characterized the derived cells, and transplanted them to irradiated mice. These cell complexes were the origin of both BM mesenchymal stem cells and various hematopoietic lineages when kept in appropriate culture conditions. [Stem Cell Dev] Abstract

Human Proangiogenic Circulating Hematopoietic Stem and Progenitor Cells Promote Tumor Growth in an Orthotopic Melanoma Xenograft Model
The authors previously identified a distinct population of human circulating hematopoietic stem and progenitor cells (CHSPCs) in the peripheral blood (PB) and bone marrow, and that their frequency in the PB can correlate with disease state. The proangiogenic subset (pCHSPC) play a role in regulating tumor progression, for they previously demonstrated a statistically significant increase in C32 melanoma growth in NOD.Cg-Prkdc scid injected with human pCHSPCs. In this paper, they provide further evidence that pCHSPCs possess proangiogenic properties. [Angiogenesis] Abstract

Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
Researchers showed that they could not find very small embryonic stem cells in mouse bone marrow with any of the reported stem cell potentials, specifically for hematopoiesis. [Stem Cell Reports]
Full Article | Press Release

CLINICAL RESEARCH

The Value of Allogeneic and Autologous Hematopoietic Stem Cell Transplantation in Prognostically Favorable Acute Myeloid Leukemia with Double Mutant CEBPA
Among 2983 patients analyzed for CEBPA mutational status treated on four HOVON/SAKK and three AMLSG protocols; 124 had acute myeloid leukemia with double mutant CEBPA and achieved first complete remission. Evaluation of the clinical impact of allogeneic and autologous hematopoietic stem cell transplantation versus chemotherapy was performed by addressing time dependency in the statistical analyses. [Blood] Abstract

First-Line Allogeneic Hematopoietic Stem Cell Transplantation of HLA-Matched Sibling Donors Compared with First-Line Ciclosporin and/or Antithymocyte or Antilymphocyte Globulin for Acquired Severe Aplastic Anemia
The authors evaluated the effectiveness and adverse events of first-line allogeneic hematopoietic stem cell transplantation of human leukocyte antigen (HLA)-matched sibling donors compared to first-line immunosuppressive therapy including ciclosporin and/or antithymocyte or antilymphocyte globulin in patients with acquired severe aplastic anemia. [Cochrane Database Syst Rev] Abstract

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Quest Diagnostics Sells Ibrutinib Royalty Rights to Royalty Pharma For $485 Million in Cash
Quest Diagnostics Incorporated announced that it has completed the sale of its rights to royalties from commercialization of the drug candidate ibrutinib to Royalty Pharma for $485 million in cash. As part of its acquisition of Celera in 2011, Quest Diagnostics gained rights to royalties on ibrutinib, an experimental cancer therapy currently in Phase III development by Pharmacyclics and Johnson & Johnson, through its Janssen Biotech subsidiary. [Quest Diagnostics Incorporated] Press Release

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Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

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Postdoctoral Position – Computational Analysis of Genome-Wide Data of Normal and Malignant Blood Cells (University of Copenhagen, Department of Biomedical Sciences)

Postdoctoral Position – Stem Cell Biology (St. Jude Children’s Research Hospital)

Postdoctoral Fellow – Cancer Stem Cell Biology (McGill University)

Postdoctoral Position – Hematopoietic Differentiation of Pluripotent Stem Cells (Center for Physiology and Pathophysiology, University of Cologne)

Research Associate (Assistant Professor) – Pediatric Hematology/Oncology (The University of Chicago)

Postdoctoral Research Fellow – Hematology/Pharmacology (Thomas Jefferson University, Cardeza Foundation for Hematologic Research)

Postdoctoral Positions – Stem Cell Biology and Epigenetics (University of Wisconsin-Madison)

Director of Cell Processing Facility (S L Collins Associates, Inc.)

Postdoctoral Researcher – Hematopoietic Stem Cells (Albert Einstein College of Medicine)

Studentship – Novel Mechanisms in Blood Cancers Drug-Resistance (University of East Anglia)

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