Hematopoiesis News Volume 4.33 | Aug 27 2013

Genome-Wide Analysis of Transcriptional Regulators in Human HSPCs Reveals a Densely Interconnected Network of Coding and Non-Coding Genes
The authors provide high-resolution genome-wide binding maps for a heptad of key transcriptional factors in human CD34⁺ hematopoietic stem/progenitor cells (HSPCs) together with quantitative RNA and microRNA expression profiles. [Blood] Abstract

β-Catenin-Independent Activation of TCF1/LEF1 in Human Hematopoietic Tumor Cells through Interaction with ATF2 Transcription Factors
Using a specific lentiviral reporter, investigators identified hematopoietic tumor cells displaying constitutive T-cell factor/lymphoid enhancer factor transcriptional activation in the absence of β-catenin stabilization. [PLoS Genet] Full Article

Transcriptional Regulation of IL-15 Expression during Hematopoiesis
Using an IL-15 reporter transgenic mouse, researchers have recently shown previously unappreciated differences in the levels of IL-15 expressed by subsets of conventional dendritic cells (CD8⁺ and CD8^–). In this study, they showed that IL-15 promoter activity was differentially regulated in subsets of hematopoietically derived cells with IL-15 expression largely limited to myeloid lineages. [J Immunol] Abstract

Covalent Immobilization of SCF and SDF1α for In Vitro Culture of Hematopoietic Progenitor Cells
Scientists immobilized a fibronectin-derived adhesive peptide sequence, RGDS; a cytokine critical in hematopoietic stem cell (HSC) self-renewal, stem cell factor (SCF); and a chemokine important in HSC homing and lodging, stromal derived factor 1α (SDF1α), onto the surfaces of poly(ethylene glycol) (PEG) hydrogels. [Acta Biomater] Abstract

CLINICAL RESEARCH

High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Rescue for Children with High-Risk Neuroblastoma
The authors compared the efficacy of myeloablative therapy with conventional therapy in children with high-risk neuroblastoma. They also determined possible effects of these interventions on adverse events, late effects and quality of life. [Cochrane Database Syst Rev] Abstract

Vascular Endothelial Growth Factor-D-Mediated Blockade of Regulatory T Cells within Tumors Is Induced by Hematopoietic Stem Cell Transplantation
Researchers analyzed the kinetics of regulatory T cells in the tumors after syngeneic hematopoietic stem cell transplantation (HSCT). Unexpectedly, the frequency of CD4⁺ cells expressing Foxp3 was increased in the spleens, whereas the frequency was clearly decreased in the tumors after HSCT. [J Immunol] Abstract

Low Tumor Burden Is Associated with Early B-Cell Reconstitution and Predictor for Outcome after Non-Myeloablative Stem Cell Transplantation for Chronic Lymphocytic Leukemia
Reconstitution, engraftment kinetics and tumor cell clearance were analyzed after reduced intensity conditioning hematopoietic cell transplantation in patients with chronic lymphocytic leukemia. Patients were transplanted from unrelated or related donors after fludarabine and 2 Gy Total Body Irradiation followed by cyclosporine and Mycophenolate Mofetil. [Leuk Lymphoma] Abstract

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Mayo Clinic to Open Stem Cell Laboratory in Phoenix
Mayo Clinic in Arizona will open its own stem cell laboratory in summer 2014. The laboratory will be initially dedicated to storing and processing stem cells used for bone marrow transplants at Mayo Clinic Hospital and Phoenix Children’s Hospital. [Mayo Foundation for Medical Education and Research] Press Release

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Postdoctoral Fellow – Gene Expression and Regulation in Stem Cell Biology (University of Texas Medical School)

Research Technologist – Human Pluripotent Stem Cell Products (STEMCELL Technologies Inc.)

Postdoctoral Position – Hematopoietic Stem Cells (St. Jude Children’s Research Hospital)

Postdoctoral Position – Hematopoiesis (Lund Stem Cell Center)

Postdoctoral Research Associates – Stem Cell Biology (University of Illinois at Chicago)

Postdoctoral Fellow – Human Stem Cell Biology and Hematopoiesis (Johns Hopkins University School of Medicine)

Tenure-Track Position – Cell Biologist (McGill University)

Postdoctoral Appointee – Cell Cycle Regulation in Tumor Stem Cells (Rutgers University, Rutgers Cancer Institute of New Jersey)

Postdoctoral Position – Stem Cell Biology (St. Jude Children’s Research Hospital)

Postdoctoral Fellow – Cancer Stem Cell Biology (McGill University)

Director of Cell Processing Facility (S L Collins Associates, Inc.)

Postdoctoral Researcher – Hematopoietic Stem Cells (Albert Einstein College of Medicine)

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