LABORATORY RESEARCH Gut Microbiota Promote Hematopoiesis to Control Bacterial Infection Researchers revealed that germ-free mice display reduced proportions and differentiation potential of specific myeloid cell progenitors of both yolk sac and bone marrow origin. Homeostatic innate immune defects may lead to impaired early responses to pathogens. [Cell Host Microbe] Abstract | Graphical Abstract | Press Release Inhibition of Endothelial ERK Signaling by Smad1/5 Is Essential for Hematopoietic Stem Cell Emergence Scientists showed that interplay of Smad1/5 and ERK signaling is essential for hemogenic endothelium-based hematopoietic stem cell emergence. Smad1/5 directly inhibits erk expression through recruiting HDAC1 to and inducing de-acetylation of the erk promoter in endothelial cells. [Nat Commun] Abstract Mechanism Governing a Stem Cell-Generating cis-Regulatory Element Scientists describe the targeted deletion of the Gata2 -3.9 switch site and mechanistically compare the -3.9 site with other GATA switch sites. The -3.9-/- mice were viable and exhibited normal Gata2 expression and steady-state hematopoiesis in the embryo and adult. [Proc Natl Acad Sci USA] Abstract Total Body Irradiation Causes Long-Term Mouse BM Injury via Induction of HSC Premature Senescence in an Ink4a- and Arf-Independent Manner Hematopoietic stem cell (HSC) defects were associated with significant increases in production of reactive oxygen species, expression of p16Ink4a and Arf mRNA, and senescence-associated β-galacotosidase activity, but not with telomere shortening or increased apoptosis, suggesting that total body irradiation induces residual bone marrow (BM) injury via induction of HSC premature senescence. [Blood] Abstract Hematopoietic MicroRNA-126 Protects against Renal Ischemia/Reperfusion Injury by Promoting Vascular Integrity Scientists hypothesized that overexpression of microRNA-126 in the hematopoietic compartment could protect the kidney against Ischemia/reperfusion injury via preservation of microvascular integrity. [J Am Soc Nephrol] Abstract Autophagy Is a Survival Mechanism of Acute Myeloid Leukemia Precursors during Dual mTORC2/mTORC1 Targeting Researchers examined whether induction of autophagy is a mechanism of leukemic cell resistance to dual mTORC1/mTORC2 inhibitors in acute myeloid leukemia leukemic progenitors. [Clin Cancer Res] Abstract Variable Correction of Artemis Deficiency by I-Sce1-Meganuclease-Assisted Homologous Recombination in Murine Hematopoietic Stem Cells Investigators used the DNA double-strand breaks introduced by the site-specific endonuclease I-Sce1 as a means of increasing homologous recombination of an exogenous DNA template in murine hematopoietic stem cells. [Gene Ther] Abstract CLINICAL RESEARCH Effects of Hematopoietic Stem Cell Transplantation on Acyl-CoA Oxidase Deficiency: A Sibling Comparison Study Researchers describe the natural history of clinical symptoms and brain imaging in two siblings with acyl-CoA oxidase deficiency, including the younger sibling’s response to allogeneic unrelated donor hematopoietic stem cell transplantation. [J Inherit Metab Dis] Abstract Plerixafor Added to G-CSF-Supported Paclitaxel-Ifosfamide-Cisplatin Salvage Chemotherapy Enhances Mobilization of Adequate Numbers of Hematopoietic Stem Cells for Subsequent Autografting in Hard-to-Mobilize Patients with Relapsed/Refractory Germ-Cell Tumors: A Single-Center Experience Investigators applied plerixafor in combination with granulocyte colony-stimulating factor (G-CSF) after salvage chemotherapy in ‘poor’ mobilizers with relapsed/refractory germ-cell tumors for high-dose chemotherapy plus autologous hematopoietic cell transplantation. [Anticancer Drugs] Abstract |