Role of Casein Kinase 1A1 in the Biology and Targeted Therapy of del(5q) MDS Investigators generated a murine model with conditional inactivation of casein kinase 1A1 gene (Csnk1a1) and found that Csnk1a1 haploinsufficiency induces hematopoietic stem cell expansion and a competitive repopulation advantage, whereas homozygous deletion induces hematopoietic stem cell failure. [Cancer Cell] Abstract | Graphical Abstract WASH Is Required for the Differentiation Commitment of Hematopoietic Stem Cells in a c-Myc-Dependent Manner Scientists show that conditional Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) deletion in the hematopoietic system causes defective blood production of the host, leading to severe cytopenia and rapid anemia. [J Exp Med] Abstract Discrete Notch Signaling Requirements in the Specification of Hematopoietic Stem Cells Researchers demonstrate that three of the four Notch receptors are independently required for the specification of hematopoietic stem cells (HSCs) in the zebrafish. The orthologs of the murine Notch1 receptor, Notch1a and Notch1b, are each required intrinsically to fate HSCs, just prior to their emergence from aortic hemogenic endothelium. [EMBO J] Abstract Quiescent Hematopoietic Stem Cells with Short Telomeres Accumulate Genomic Damage but Are Blocked from Hematopoietic Activation by Senescence and Apoptosis Scientists show that telomere shortening results in DNA damage accumulation and gene expression changes in quiescent hematopoietic stem cells (HSCs) of aged mice. Upon activation, a subset of HSCs with elevated levels of DNA damage and p16 expression is blocked from cell cycle entry and apoptosis is induced in HSCs entering cell cycle. [Blood] Abstract Requirement for Ssbp2 in Hematopoietic Stem Cell Maintenance and Stress Response Investigators provide several lines of evidence to demonstrate a requirement for Ssbp2 in the function and transcriptional program of hematopoietic stem and progenitor cells in vivo. [J Immunol] Abstract Prep1 (pKnox1) Regulates Mouse Embryonic HSC Cycling and Self-Renewal Affecting the Stat1-Sca1 IFN-Dependent Pathway Scientists demonstrate that Prep1 regulates the number of hematopoietic stem cells in fetal livers, their clonogenic potential and their ability to de novo generate the hematopoietic system in ablated hosts. [PLoS One] Full Article Leukemic Stem Cell Frequency: A Strong Biomarker for Clinical Outcome in Acute Myeloid Leukemia The nature of the putative leukemic stem cells and normal hematopoietic stem cells, present in the same patient’s bone marrow, was demonstrated in eight patients by the presence or absence of molecular aberrancies and/or leukemic engraftment in NOD-SCID IL-2Rγ-/- mice. [PLoS One] Full Article CLINICAL RESEARCH Bone Marrow Graft as a Source of Allogeneic Hematopoietic Stem Cells in Patients Undergoing a Reduced Intensity Conditioning Regimen In an attempt to reduce the incidence of chronic GvHD after reduced-intensity conditioning, investigators used BM instead of PBSC and added melphalan 100 mg/m2 to the classical association of fludarabine, 30 mg/m2/day for three days and TBI, 200 cGy. [Bone Marrow Transplant] Abstract Impact of Pre-Transplantation Disease Burden on the Outcome of Allogeneic Hematopoietic Stem Cell Transplantation in Refractory and Relapsed AML: A Single-Center Study Patients with refractory or relapsed acute myeloid leukemia (AML) receiving allogeneic hematopoietic stem cell transplantation, were retrospectively assessed to evaluate the effect of disease burden on outcomes and to identify predictive variables. [Leuk Lymphoma] Abstract Treosulfan-Based Conditioning Regimens for Allogeneic HSCT in Children with Acute Lymphoblastic Leukemia Researchers retrospectively studied toxicity and outcome of 71 pediatric patients with acute lymphoblastic leukemia undergoing hematopoietic stem cell transplantation (HSCT) following treosulfan-based conditioning aiming to identify risk factors for treatment failure and dose-depending outcome differences. [Ann Hematol] Abstract |