Hematopoiesis News Volume 6.10 | Mar 17 2015

Shedding of Clinical-Grade Lentiviral Vectors Is Not Detected in a Gene Therapy Setting
Researchers investigated factors influencing the extent of lentiviral vector shedding upon ex vivo transduction of human hematopoietic stem and progenitor cells. [Gene Ther] Abstract

Hematopoietic Stem Cell Gene Therapy Corrects the Neuropathic Phenotype in Murine Model of Mucopolysaccharidosis Type II
Investigators showed that lentiviral isogeneic hematopoietic stem cell gene therapy corrects the neuronal manifestation through the amelioration of lysosomal storages and autophagic dysfunctions in brains from mucopolysaccharidosis type II mice. [Hum Gene Ther] Abstract

Expression of Coxsackievirus and Adenovirus Receptor Separates Hematopoietic and Cardiac Progenitor Cells in Fetal Liver Kinase 1-Expressing Mesoderm
Scientists found that mouse and human pluripotent stem cell- and mouse embryo-derived fetal liver kinase 1 (Flk1)⁺ cells could be subdivided into Flk1⁺ coxsackievirus and adenovirus receptor (CAR)⁺ cells and Flk1⁺CAR^– cells. The progenitor cells with cardiac potential were almost entirely restricted to Flk1⁺CAR⁺ cells, and Flk1⁺CAR^– cells efficiently differentiated into hematopoietic cells. [Stem Cells Transl Med] Abstract

Pluripotent Cell Models of Fanconi Anemia Identify the Early Pathological Defect in Human Hemoangiogenic Progenitors
Researchers established induced pluripotent stem cells (iPSCs) from fibroblasts of six patients with Fanconi anemia (FA) and FANCA mutations. Quantitative evaluation of the differentiation ability demonstrated that the differentiation propensity toward the hematopoietic and endothelial lineages is already defective in early hemoangiogenic progenitors. [Stem Cells Transl Med] Abstract

Dual Roles of Autologous CD8⁺ T Cells in Hematopoietic Progenitor Cell Mobilization and Engraftment
Scientists assessed the effect of purified autologous CD8⁺ T cells from low and high mobilizers on hematopoietic progenitor cell migration from high to low stromal cell-derived factor concentration gradients. [Transfusion] Abstract

CLINICAL RESEARCH

Phase I Study of a Bispecific Ligand-Directed Toxin Targeting CD22 and CD19 (DT2219) for Refractory B-Cell Malignancies
Investigators conducted a Phase I dose-escalation study to assess the safety, maximum tolerated dose, and preliminary efficacy of DT2219 in patients with relapsed/refractory B-cell lymphoma or leukemia. [Clin Cancer Res] Abstract | Press Release

Strategies to Inhibit Alloantibody Production in Alloprimed Murine Recipients of Hematopoietic Stem Cell Grafts
Researchers showed that a single intravenous injection of donor splenocytes, to mimic a blood transfusion, results in high, sustained levels of serum alloantibody sufficient to eliminate donor bone marrow within three hours, resulting in uniform mortality in lethally irradiated allogeneic recipients. [Am J Transplant] Abstract

The Impact of Graft-versus Host Disease Prophylaxis in Reduced-Intensity Conditioning Allogeneic Stem Cell Transplant in Acute Myeloid Leukemia: A Study from the Acute Leukemia Working Party of EBMT
The impact of the intensity of graft-versus-host-disease immunoprophylaxis on transplantation outcomes in patients undergoing transplantation following reduced-intensity conditioning is unclear. Investigators address this question in 228 adult patients above 50 years of age with acute myeloid leukemia in first complete remission given peripheral blood stem cells from HLA-identical siblings after fludarabine and two days of intravenous busulfan reduced-intensity conditioning. [Haematologica] Abstract | Full Article

Deregulation of Innate Immune and Inflammatory Signaling in Myelodysplastic Syndromes
The deregulation of innate immune and inflammatory signaling should be considered one of the driving forces in the pathogenesis of MDS. The authors review and update the advances in this field, summarizing the results from the most recent studies and discussing their clinical implications. [Leukemia] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.

From our sponsor: Free mouse hematopoietic progenitors wallchart. Request your copy.

The Alliance for Regenerative Medicine Enters into Memorandum of Understanding with Japan’s Forum for Innovative Regenerative Medicine
The Alliance for Regenerative Medicine announced it has entered into a memorandum of understanding with Japan’s Forum for Innovative Regenerative Medicine, effective immediately. [The Alliance for Regenerative Medicine] Press Release

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

Visit our events page to see a complete list of events in the hematopoiesis community.

Research Technologist – Hematopoietic Product Development (STEMCELL Technologies Inc.)

Principal Scientist – Hematopoietic Products (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Research Scientist – Cell Signaling in Normal and Leukemic Hematopoietic Stem and Progenitor Cells (Indiana University)

Postdoctoral Fellow – Stem Cell/Translational Medicine (Rutgers New Jersey Medical School)

Postdoctoral Position – Drug Development for Anemia (Lund University)

Postdoctoral Position – Pediatric Hematology and Oncology (University Medical Center of Freiburg)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

Director – Vector Production (Sangamo BioSciences, Inc.)

Postdoctoral Position – Genetic Engineering and Transplantation of Hematopoietic Stem Cells (University of California – San Francisco)

Recruit Top Talent: Reach more than 60,000 potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.