Combined Single-Cell Functional and Gene Expression Analysis Resolves Heterogeneity within Stem Cell Populations To gain deeper insight into the gene expression program of murine hematopoietic stem cells, researchers combined single-cell functional assays with flow cytometric index sorting and single-cell gene expression assays. [Cell Stem Cell] Full Article | Graphical Abstract | Press Release Dissecting the Role of Aberrant DNA Methylation in Human Leukemia The authors showed that, by applying cellular reprogramming to primary chronic myeloid leukemia cells, aberrant DNA methylation contributes to the disease evolution. Using a BCR-ABL inducible murine model, they demonstrated that a single oncogenic lesion triggers DNA methylation changes, which in turn act as a precipitating event in leukemia progression. [Nat Commun] Abstract Mouse Prenatal Platelet-Forming Lineages Share a Core Transcriptional Program but Divergent Dependence on MPL Researchers performed a comprehensive study to determine the spatial and temporal appearance of prenatal platelet-forming lineages. They demonstrated that diploid platelet-forming cells originate in the yolk sac and then rapidly migrate to other extra- and intra-embryonic tissues. [Blood] Abstract Disruption of the 5S RNP-Mdm2 Interaction Significantly Improves the Erythroid Defect in a Mouse Model for Diamond-Blackfan Anemia Scientists crossed their mouse model for RPS19-deficient Diamond-Blackfan anemia with Mdm2C305F knock-in mice that have a disrupted 5S RNP-Mdm2 interaction. Upon induction of the Rps19 deficiency, Mdm2C305F reversed the p53 response and improved expansion of hematopoietic progenitors in vitro, and ameliorated the anemia in vivo. [Leukemia] Abstract Tissue Engineered Humanized Bone Supports Human Hematopoiesis In Vivo Investigators describe a highly reproducible method to engineer humanized bone constructs that are able to recapitulate the morphological features and biological functions of the hematopoietic stem cell niches. [Biomaterials] Abstract Differential Hematopoietic Activity in White Adipose Tissue Depending on Its Localization Researchers investigated whether hematopoietic stem/progenitor cell can be found in visceral adipose tissue (VAT) and whether a putative difference in in situ hematopoiesis may be related to anatomical location and to site-specific immune cell content in VAT compared to subcutaneous adipose tissue. [J Cell Physiol] Abstract G-CSF Induces Osteoblast Inhibition by B Lymphocytes and Osteoclast Activation by T Lymphocytes during Hematopoietic Stem/Progenitor Cells Mobilization Investigators examined whether T and B lymphocytes modulate the bone niche and influence hematopoietic stem and progenitor cell (HSPC) mobilization. They used T and B defective mice to show that G-CSF induced mobilization of HSPCs correlated with B lymphocyte, but poorly with T lymphocyte. [Biol Blood Marrow Transplant] Abstract Endoglin Potentiates Nitric Oxide Synthesis to Enhance Definitive Hematopoiesis To evaluate whether high endoglin (ENG) expression accelerates hematopoiesis, scientists generated an inducible ENG expressing ES cell line and forced expression in FLK1+ mesodermal or TIE2+/CD117+ hemogenic endothelium cells. [Biol Open] Abstract | Full Article CLINICAL RESEARCH Impact of Pre-Transplant FDG-PET Status on Outcomes after Allogeneic Hematopoietic Cell Transplantation for Non-Hodgkin Lymphoma Investigators assessed with 18F-fluorodeoxy glucose-positron emission tomography (FDG-PET) prior to hematopoietic cell transplantation for lymphoma may be prognostic for outcomes. [Biol Blood Marrow Transplant] Abstract Complement-Binding Donor-Specific Anti-HLA Antibodies and Risk of Primary Graft Failure in Hematopoietic Stem Cell Transplantation Researchers hypothesized that complement-binding donor-specific anti-HLA antibodies (DSA) are associated with engraftment failure in hematopoietic stem-cell transplantation and analyzed 122 haploidentical transplant recipients tested prospectively for DSA. [Biol Blood Marrow Transplant] Abstract |