Reticular Dysgenesis-Associated AK2 Protects Hematopoietic Stem and Progenitor Cell Development from Oxidative Stress. In zebrafish, adenylate kinase (AK)2 deficiency affected hematopoietic stem and progenitor cell development with increased oxidative stress and apoptosis. Antioxidant treatment rescued the hematopoietic phenotypes in vivo in ak2 mutant zebrafish and restored differentiation of AK2-deficient induced pluripotent stem cells into mature granulocytes. [J Exp Med] Abstract miR-139-5p Controls Translation in Myeloid Leukemia through EIF4G2 Researchers identified microRNA (miR)-139-5p suppression across several cytogenetically defined acute myeloid leukemia (AML) subgroups. Restoration of mir-139 expression in cell lines representing the major AML subgroups caused cell cycle arrest and apoptosis in vitro and in xenograft mouse models in vivo. [Oncogene] Abstract The Oncofusion Protein FUS–ERG Targets Key Hematopoietic Regulators and Modulates the All-trans Retinoic Acid Signaling Pathway in t(16;21) Acute Myeloid Leukemia Researchers showed that FUS–ERG acts in the context of a heptad of proteins central to proper expression of genes involved in maintaining a stem cell hematopoietic phenotype. [Oncogene] Full Article Inhibition of Thrombopoietin/Mpl Signaling in Adult Hematopoiesis Identifies New Candidates for Hematopoietic Stem Cell Maintenance The authors constructed an intracellular-truncated, signaling-deficient Mpl protein which is presented on the cell surface. The transplantation of bone marrow cells retrovirally transduced to express dnMpl into wildtype mice induced thrombocytopenia, and a progressive loss of hematopoietic stem cell. [PLoS One] Full Article Modulation of Hematopoietic Progenitor Cell Fate In Vitro by Varying Collagen Oligomer Matrix Stiffness in the Presence or Absence of Osteoblasts To recreate the in vivo hematopoietic cell microenvironment or niche and to study the impact of extracellular matrix biophysical properties on hematopoietic progenitor cell (HPC) proliferation and function, mouse bone-marrow derived HPCs were cultured within three-dimensional type I collagen oligomer matrices. [J Immunol Methods] Abstract A Quantitative Assessment of the Content of Hematopoietic Stem Cells in Mouse and Human Endosteal-Bone Marrow: A Simple and Rapid Method for the Isolation of Mouse Central Bone Marrow A quantitative and qualitative assessment of cell populations isolated from mouse and human bone marrow was undertaken with a focus on the distribution of hematopoietic cells between the central bone marrow and endosteal bone marrow. [BMC Hematol] Full Article CLINICAL RESEARCH Allogeneic Hematopoietic Stem Cell Transplantation for Poor-Risk CLL: Dissecting Immune-Modulating Strategies for Disease Eradication and Treatment of Relapse To elucidate factors contributing to the effectiveness of allogeneic hematopoietic stem cell transplantation in high-risk CLL, immune interventions, GvHD and clinical outcome of 77 consecutive patients allografted for CLL were analyzed. [Bone Marrow Transplant] Abstract Allogeneic Hematopoietic Stem Cell Transplantation in Adults with Myelodysplastic Syndrome: Experience of the Argentinean Group of Bone Marrow Transplantation (GATMO) In this multicenter retrospective study, investigators analyzed the outcome of adult patients with myelodysplastic syndrome who underwent allogeneic hematopoietic stem cell transplantation in Argentina and evaluated the prognostic factors associated with progression-free survival, overall survival, cumulative incidence of relapse, and non-relapse mortality. [Hematology] Abstract |