Hematopoiesis News Volume 6.37 | Sep 22 2015

Single-Cell Transcriptomic Reconstruction Reveals Cell Cycle and Multi-Lineage Differentiation Defects in Bcl11a-Deficient Hematopoietic Stem Cells
Investigators employed single-cell transcriptomic analysis to dissect the molecular heterogeneities in hematopoietic stem cells (HSCs). Analysis of lineage gene expression suggests that the Bcl11a^-/- HSCs are constituted of two distinct myeloerythroid-restricted subpopulations. [Genome Biol] Full Article

Hematopoietic Stem Cells Develop in the Absence of Endothelial Cadherin 5 Expression
Utilizing time-lapse confocal imaging, parabiotic surgical pairing of zebrafish embryos, and blastula transplantation assays, researchers showed that hematopoietic stem cells emerge, migrate, engraft, and differentiate in the absence of cadherin 5 expression. [Blood] Abstract

ATF4 Plays a Pivotal Role in the Development of Functional Hematopoietic Stem Cells in Mouse Fetal Liver
Investigators demonstrated that deletion of activating transcription factor 4 (ATF4) significantly impaired hematopoietic development and reduced hematopoietic stem cell self-renewal in fetal liver. [Blood] Abstract

Functional Niche Competition Between Normal Hematopoietic Stem and Progenitor Cells and Myeloid Leukemia Cells
Scientists used the MLL-AF9 murine acute myeloid leukemia in a competitive repopulation model to investigate whether normal hematopoietic stem and progenitor cells and leukemic cells functionally compete for the same marrow niches. [Stem Cells] Abstract

Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
Researchers demonstrated that hematopoietic progenitor cell homing occurs earlier than previously reported and that hematopoietic progenitor cells reside in muscle tissue before bone marrow hematopoiesis occurs during mouse embryogenesis. [PLoS One] Full Article

Integration of Shh and Wnt Signaling Pathways Regulating Hematopoiesis
Researchers investigated the spatial and temporal programmed expression of Shh and Wnt members during key stages of definitive hematopoiesis and the possible mechanism of Shh and Wnt signaling pathways regulating the proliferation of hematopoietic progenitor cells. [DNA Cell Biol] Abstract

CLINICAL RESEARCH

Impact of MRD and TKI on Allogeneic Hematopoietic Cell Transplantation for Ph⁺ALL: A Study from the Adult ALL WG of the JSHCT
The authors retrospectively analyzed data from a registry database for 432 adult Ph-positive ALL (Ph⁺ALL) patients in first CR who received pre-transplant tyrosine kinase inhibitor administration. [Bone Marrow Transplant] Abstract

Should Busulfan Therapeutic Range Be Narrowed in Pediatrics? Experience from a Large Cohort of Hematopoietic Stem Cell Transplant Children
Researchers investigated the relevance of using a more restricted therapeutic range and investigated the association between busulfan therapeutic range and clinical outcome. [Bone Marrow Transplant] Abstract