Stable Multilineage Xenogeneic Replacement of Definitive Hematopoiesis in Adult Zebrafish Investigators used a zebrafish strain lacking definitive hematopoiesis for interspecific analysis of hematopoietic cell development. Without conditioning prior to transplantation, hematopoietic progenitor cells from goldfish stably engraft in adult zebrafish homozygous for the c-mybI181N mutation. [Sci Rep] Full Article Co-Cultured hBMSCs and HUVECs on Human Bio-Derived Bone Scaffolds Provide Support for the Long-Term Ex Vivo Culture of HSC/HPCs In order to closely mimic a multi-cell state in hematopoietic stem/progenitor cells (HSC/HPCs) vascular niche, researchers co-cultured human bone marrow mesenchymal stem cells (hBMSCs) and human umbilical vein endothelial cells (HUVECs) without any cytokines as feeder cells and applied bio-derived bone from human femoral metaphyseal portion as scaffold to develop a new HSC/HPCs three-dimensional culture system. [J Biomed Mater Res A] Abstract In Vitro and In Vivo Evaluation of Cord Blood Hematopoietic Stem and Progenitor Cells Amplified with Glycosaminoglycan Mimetic Because glycosaminoglycans (GAGs) are known to be important components of the hematopoietic niche and to modulate growth factor effects, investigators explored the use of GAG mimetic OTR4131 to potentiate hematopoietic stem cells and progenitor cells in vitro expansion and in vivo engraftment. [Stem Cell Res Ther] Full Article Increased Engraftment of Human Short Term Repopulating Hematopoietic Cells in NOD/SCID/IL2rγnull Mice by Lentiviral Expression of NUP98-HOXA10HD Researchers evaluated whether NUP98-HOXA10HD could be used to expand engrafting human primitive CD34+ cells in an immunodeficient mouse model. [PLoS One] Full Article Reprogramming of Human Peripheral Blood Monocytes to Erythroid Lineage by Blocking of the PU-1 Gene Expression To demonstrate whether PU.1 suppression induces monocyte lineage conversion into red blood cells, a combination of three PU.1-specific siRNAs was implemented to knock down PU.1 gene expression and generate the balance in favor of GATA-1 expression to induce erythroid differentiation. [Ann Hematol] Abstract ESAM Is a Novel Human Hematopoietic Stem Cell Marker Associated with a Subset of Human Leukemias Researchers found that endothelial cell-selective adhesion molecule (ESAM) can also be used to purify human hematopoietic stem cells from all the currently available sources (adult bone marrow, mobilized peripheral blood, and cord blood). [Exp Hematol] Abstract Ezh2 Regulates the Lin28/let-7 Pathway to Restrict Activation of Fetal Gene Signature in Adult Hematopoietic Stem Cells Scientists showed that in the absence of the polycomb-group gene Ezh2, a cohort of fetal-specific genes, including let-7 target genes, were activated in bone marrow (BM) hematopoietic stem and progenitor cells (HSPCs), leading to acquisition of fetal phenotypes by BM HSPCs, such as enhanced self-renewal activity and production of fetal-type lymphocytes. [Exp Hematol] Abstract CLINICAL RESEARCH Global Use of Peripheral Blood vs Bone Marrow as Source of Stem Cells for Allogeneic Transplantation in Patients with Bone Marrow Failure The authors used international survey and transplant registry data to compare use of bone marrow with use of peripheral blood stem cells for hematopoietic stem cell transplantation in World Health Organization member countries. [JAMA] Abstract | Press Release Analysis of Non-Relapse Mortality and Causes of Death over 15 Years following Allogeneic Hematopoietic Stem Cell Transplantation Investigators performed a retrospective single-center study to assess changes in outcomes after allogeneic hematopoietic stem cell transplantation and causes of non-relapse mortality over three five year periods. [Bone Marrow Transplant] Abstract |