Telomerase Gene Therapy Rescues Telomere Length, Bone Marrow Aplasia and Survival in Mice with Aplastic Anemia Investigators tested the therapeutic efficacy of telomerase activation by using AAV9 gene therapy vectors carrying the telomerase Tert gene in two independent mouse models of aplastic anemia owing to short telomeres. They found that a high dose of AAV9-Tert targets the bone marrow compartment including hematopoietic stem cells. [Blood] Abstract Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation Scientists generated global gene expression, chromatin accessibility, histone modification, and transcription factor binding data from purified embryonic stem cell-derived cells representing six sequential stages of hematopoietic specification and differentiation. [Dev Cell] Full Article | Graphical Abstract | Press Release MEF2C and EBF1 Co-Regulate B Cell-Specific Transcription Researchers identified early B cell factor-1 (EBF1) as a co-regulator of gene expression with myocyte enhancer factor 2C (MEF2C). A genome-wide survey of MEF2C and EBF1 binding sites identified a subset of B cell-specific genes that they target. [PLoS Genet] Full Article Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Contributes to the Proliferation of Hematopoietic Progenitor Cells in Murine Bone Marrow via PACAP-Specific Receptor The authors assessed the expression of a PACAP-specific receptor (PAC1) gene and protein in mouse bone marrow. Cells strongly expressing PAC1+ were large in size, had oval nuclei, and merged with CD34+ cells, suggesting that the former were hematopoietic progenitor cells. Compared with wild-type mice, adcyap1−/− mice exhibited lower multiple potential progenitor cell populations and cell frequency in the S-phase of the cell cycle. [Sci Rep] Full Article Hyper-Active Non-Homologous End Joining Selects for Synthetic Lethality Resistant and Pathological Fanconi Anemia Hematopoietic Stem and Progenitor Cells Investigators showed that poly[ADP-ribose] polymerase 1 activation in hematopoietic stem and progenitor cells (HSPCs) in response to genotoxic or oxidative stress attenuates HSPC exhaustion. [Sci Rep] Full Article BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo Researchers found that the aorta-gonad-mesonephros (AGM) harbors two types of adult-repopulating hematopoietic stem cells (HSC) upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. [Stem Cell Reports] Full Article | Graphical Abstract H2S Protects against Fatal Myelosuppression by Promoting the Generation of Megakaryocytes/Platelets H2S promoted the generation of megakaryocytes, increased platelet levels, ameliorate entorrhagia, and improved survival. These H2S effects were blocked in both in vivo and in vitro models with thrombopoietin receptor knockout mice. [J Hematol Oncol] Full Article CLINICAL RESEARCH Allogeneic Hematopoietic Cell Transplantation for Patients with Mixed Phenotype Acute Leukemia Researchers analyzed the outcome of 95 carefully characterized allogeneic hematopoietic stem cell transplant patients with mixed phenotype acute leukemia reported to the Center for International Blood and Marrow Transplant Research between 1996 and 2012. [Biol Blood Marrow Transplant] Abstract Alemtuzumab-BEAM as Conditioning for Allogeneic Hematopoietic Stem Cell Transplantation in Relapsed/Refractory Hodgkin Lymphoma: A Single-Center Analysis The authors retrospectively analyzed the outcome of 20 patients with relapsed/refractory Hodgkin’s lymphoma who received allogeneic hematopoietic stem cell transplantation following conditioning therapy with alemtuzumab-BEAM. [J Cancer Res Clin Oncol] Abstract |