Hematopoiesis News Volume 7.18 | May 10 2016

Mesenchymal Stromal Cell Derived Extracellular Vesicles Rescue Radiation Damage to Murine Marrow Hematopoietic Cells
The authors evaluated the capacity of mesenchymal stromal cells-derived extracellular vesicles to mitigate radiation injury to marrow stem cells at four hours to seven days after irradiation. [Leukemia] Abstract

Xpg Limits the Expansion of Hematopoietic Stem and Progenitor Cells after Ionizing Radiation
Scientists screened for genome stability factors negatively impacting maintenance of hematopoietic stem cells (HSCs) in the context of ionizing radiation (IR). They found that in vivo knock down of Xeroderma pigmentosum, complementation group G (Xpg) caused elevation of HSC numbers after IR treatment, while numbers of hematopoietic progenitors are elevated to a lesser extent. [Nucleic Acids Res] Full Article

Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities
To better understand functional interplay between trisomy 21 and GATA1 mutations in hematopoiesis, scientists constructed cellular disease models using human induced pluripotent stem cells (iPSCs) and genome-editing technologies. Comparative analysis of these engineered iPSCs demonstrated that trisomy 21 perturbed hematopoietic development through the enhanced production of early hematopoietic progenitors and the upregulation of mutated GATA1. [Cell Rep] Full Article | Graphical Abstract

RNA Polymerase III Component Rpc9 Regulates Hematopoietic Stem and Progenitor Cell Maintenance in Zebrafish
Researchers isolated a zebrafish mutant with defective T lymphopoiesis and positional cloning identified that Rpc9, a component of DNA-directed RNA polymerase III complex, is responsible for the mutant phenotype. [Development] Abstract

Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells
Scientists showed the method of measuring the ATP levels in hematopoietic stem cells (HSCs) sorted from murine bone marrow. They used magnetic sorting as well as a cell sorter and adopted the commonly used bioluminescence-based detection kits in described protocol. Their strategy allows to measure ATP levels in 1000 highly purified HSC. [Methods Mol Biol] Abstract

Deorphanization and Characterization of the Ectopically Expressed Olfactory Receptor OR51B5 in Myelogenous Leukemia Cells
Scientists detected the expression of OR51B5 in the chronic myelogenous leukemia cell line K562 and in white blood cell samples of clinically diagnosed acute myelogenous leukemia patients by reverse transcription-PCR and immunocytochemical staining. [Cell Death Disc] Full Article

CLINICAL RESEARCH

Allogeneic Transplantation Is Not Superior to Chemotherapy in Most Patients over 40 Years of Age with Philadelphia-Negative Acute Lymphoblastic Leukemia in First Remission
The authors retrospectively compared the outcome of patients >40 years treated with hematopoietic stem-cell transplantation (HSCT) or chemotherapy alone in first remission. Three-year overall survival and disease-free survival were not significantly different between the chemotherapy-only and HSCT groups [Am J Hematol] Abstract

Use of an Oral Busulfan Test Dose in Patients Undergoing Hematopoietic Stem Cell Transplantation Treated with or without Fludarabine
Researchers investigated the importance of an oral test dose for busulfan (BU) dose adjustment before conditioning regimen for hematopoietic stem cell transplantation and the effect of fludarabine on the oral BU pharmacokinetics evaluated after the 5^th treatment dose. [J Clin Pharmacol] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.

FDA Expands IMBRUVICA^® (Ibrutinib) Label to Include New Data from Two Key Phase III Trials, Adding Overall Survival and Combination Data
The U.S. Food and Drug Administration (FDA) has approved an expansion to the IMBRUVICA^® U.S. Prescribing Information based on data supporting its use in patients with chronic lymphocytic leukemia/small lymphocytic lymphoma, Janssen announced. [Janssen Global Services, LLC] Press Release

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Visit our events page to see a complete list of events in the hematopoiesis community.

NEW Postdoctoral Fellow – Leukemia Lymphoma (Emory University)

Scientist – Hematology (STEMCELL Technologies Inc.)

Postdoctoral Scientist – Mouse Hematopoiesis (Lund University)

Postdoctoral Fellow – Hematopoietic Stem Cell Biology and Blood Disorders (Brigham & Women’s Hospital)

Research Technician – Hematopoietic Stem Cell Biology (Fred Hutchinson Cancer Research Center)

Postdoctoral Research Associate – Stem Cell Aging Biology (University of Southern California)

Postdoctoral Fellow – Hematopoietic Stem Cells (University of Pennsylvania)

Postdoctoral Fellow – Hematology/Oncology (Boston Children’s Hospital)

Assistant/Associate/Full Member Faculty – Myeloid Malignancies (Fred Hutchinson Cancer Research Center)

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