Hepatic Cell News 3.45 November 29, 2019 | |
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TOP STORYResearchers screened aggressive human hepatocellular carcinoma (HCC) for macroH2A1 and CD44 cancer stem cell marker expression. They also knocked down macroH2A1 in HCC cells, and performed integrated transcriptomic and secretomic analyses. [Theranostics] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)HBsAg-Specific CD8+ T Cells as an Indispensable Trigger to Induce Murine Hepatocellular Carcinoma Recipient hepatocytes mice exhibited persistent HBsAg expression with chronic hepatitis and eventually developed hepatocellular carcinoma (HCC) with a prevalence of 100%. HBsAg-specific CD8+ T cells were generated and specifically and continuously induced hepatocyte apoptosis with progressive chronic inflammation, and the depletion of CD8+ T cells or their deficiency prevented HCC, which could then be reproduced by the transfer of HBsAg-specific CD8+ T cells. [Cell Mol Immunol] Abstract Hepatocellular carcinoma tissue samples were used to examine the association between DNMTs/OCT4 expression levels and clinical prognosis. Serum levels of IL-6 were detected using ELISA assays. Gain- and loss-of-function experiments were performed in cell lines and mouse xenograft models to determine the underlying mechanism in vitro and in vivo. [J Exp Clin Cancer Res] Full Article Levels of FOXG1 were determined by immunohistochemical and real-time PCR analysis in hepatocellular carcinoma (HCC) cell lines and human HCC samples. The effect of FOXG1 on cancer cell invasion and metastasis was investigated in vitro and in vivo in either FOXG1-silenced or overexpressing human HCC cell lines. [J Exp Clin Cancer Res] Full Article Mechanistically, MKL1 was recruited to the promoter region of TWIST1, a master regulator of endothelial-mesenchymal transition, and activated TWIST1 transcription in a STAT3-dependent manner. A small-molecule STAT3 inhibitor alleviated EndMT in cultured cells and bile duct ligation induced liver fibrosis in mice. [Cell Death Dis] Full Article CircRNA expression profile was analyzed by a microarray. Regulatory gene targets were predicted by bioinformatics analysis and validated by luciferase assay. Their expression was determined by qRT-PCR and Western blotting. [Cell Death Dis] Full Article Glutamine/Glutamate Metabolism Rewiring in Reprogrammed Human Hepatocyte-Like Cells The authors report, using stable isotope-resolved metabolic analysis in combination with metabolomic-lipidomic approaches, that hepatocyte-like (HEP-L) cells mirrored glutamine/glutamate metabolism in primary cultured human hepatocytes that was very different from parental human fibroblasts. HEP-L cells diverged glutamine from multiple metabolic pathways into deamidation and glutamate secretion, just like periportal hepatocytes did. [Sci Rep] Full Article Researchers found that a mutation of alternative p38 in mice augmented the severity of acute liver inflammation. Moreover, TNF-induced hepatocyte death was augmented by a mutation of alternative p38, suggesting that alternative p38 signaling in hepatocytes contributed more significantly to the pathology of acute liver injury. [Sci Rep] Full Article The effect of a novel semi-natural derivative of naringenin, 6-C-(E-phenylethenyl)naringenin (6-CEPN) on hepatocellular carcinoma (HCC) stemness was evaluated both in vitro and in vivo. 6-CEPN reduced HCC cell viability, inhibited sphere formation, cell migration and invasion, and blocked epithelial-mesenchymal-transition. [J Agric Food Chem] Abstract Subscribe to one of our other 19 science newsletters such as Intestinal Cell News & Pancreatic Cell News. | |
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REVIEWSThe authors collate evidence of the regulatory role of miRNA in each stage of the HBV-HCC continuum. This includes the regulatory role of miRNA in early HBV infection, chronic inflammation, fibrosis/cirrhosis, and the onset of HCC. [Cells] Full Article | Graphical Abstract The Many Roles of Cell Adhesion Molecules in Hepatic Fibrosis Investigators focus on selectins, cadherins, integrins and members of the immunoglobulin superfamily of adhesion molecules as well as some non-classical cell adhesion molecules in the context of hepatic fibrosis. They describe their liver-specific contributions to leukocyte recruitment, cell differentiation and survival, matrix remodeling or angiogenesis and touch on their suitability as targets in antifibrotic therapies. [Cells] Full Article Visit our reviews page to see a complete list of reviews in the hepatic cell research field. | |
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INDUSTRY NEWSGENFIT announced that the Data Safety Monitoring Board (DSMB) issued a positive recommendation for the continuation, without any modifications, of the RESOLVE-IT Phase III trial evaluating elafibranor in NASH. [GENFIT] Press Release Algernon Pharmaceuticals Provides Update on Its Planned Phase II Clinical Trial Algernon Pharmaceuticals Inc. has acquired the capital resources required to launch its first Phase II clinical trial using one of its lead drug compounds in one of its four identified key disease areas being non-alcoholic steatohepatitis, chronic kidney disease, inflammatory bowel disease and idiopathic pulmonary fibrosis. [Algernon Pharmaceuticals Inc. (GlobeNewswire, Inc.)] Press Release Intercept Pharmaceuticals, Inc. announced that the FDA has accepted Intercept’s New Drug Application (NDA) for obeticholic acid (OCA) seeking accelerated approval for the treatment of fibrosis due to nonalcoholic steatohepatitis (NASH) and granted priority review. [Intercept Pharmaceuticals, Inc.] Press Release | |
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POLICY NEWSPhD Students in China Report Misery and Hope In a Nature survey, only 55% of the Chinese students who responded to the survey said that they were at least partially satisfied with their PhD experience. For the 5,630 respondents outside China, the satisfaction rate was 72%. [Nature News] Editorial Chinese Ministry Investigates Duplications in Papers by University President The Chinese education ministry is investigating scientific articles authored by high-profile immunologist and university president Cao Xuetao, following suggestions that dozens of papers contain potentially problematic images. Four journals also say they are examining papers from Cao. [Nature News] Editorial Stark Party Differences in ‘Brexit Election’ Could Shape Future of UK Science When representatives of each party took to a stage last week at the Royal Society in London, they all promised increased funding and easier immigration for scientists. But Brexit loomed over the discussion, says Sarah Main, director of the Campaign for Science and Engineering, an advocacy group in London. [ScienceInsider] Editorial
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EVENTSNEW Advancing Precision Medicine Drug Development: Incorporation of Real-World Data and Other Novel Strategies Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Researcher – HIV and the Liver (Seattle’s Children’s Hospital) Postdoctoral Researcher – Oncogenic Pathways (University of Liège) Faculty Member – Pathogen-Associated Malignancies (Fred Hutchinson Cancer Research Center) Postdoctoral Fellow – Drug-Induced Liver Injury (Johnson & Johnson) Postdoctoral Researcher – Liver Disease Treatment (University of Southern Denmark) Postdoctoral Researcher – Personalized Medicine (Mayo Clinic Center for Individualized Medicine) Postdoctoral Researcher – Liver Plasticity (University of Southern Denmark) Postdoctoral Scientist – RAS Cancer Biology (Genetech, Inc.) Assistant Professor – Liver Research (The University of Hong Kong) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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