Functional Analysis via Standardized Whole-Blood Stimulation Systems Defines the Boundaries of a Healthy Immune Response to Complex Stimuli The authors have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, the researchers defined the protein signatures induced by: medically relevant bacteria, fungi, and viruses; agonists specific for defined host sensors; clinically employed cytokines; and activators of T cell immunity. [Immunity] Abstract | Press Release Recognition and Killing of Autologous, Primary Glioblastoma Tumor Cells by Human Cytomegalovirus pp65-Specific Cytotoxic T Cells Researchers explored whether T cells stimulated by cytomegalovirus pp65 RNA-transfected dendritic cells target and eliminate autologous glioblastoma multiforme tumor cells in an antigen-specific manner. [Clin Cancer Res] Abstract Invariant Natural Killer T Cells Developing in the Human Fetus Accumulate and Mature in the Small Intestine Scientists showed that functional human fetal invariant natural killer T cells develop and differentiate in a tissue-specific manner during the second trimester. [Mucosal Immunol] Abstract Manipulating the Expression of Chemokine Receptors Enhances Delivery and Activity of Cytokine-Induced Killer Cells Human cytokine-induced killer cell expansions were examined for the level, profile and kinetics of chemokine receptor expression. [Br J Cancer] Abstract Human Monocytes Respond to Extracellular cAMP through A2A and A2B Adenosine Receptors Researchers tested the hypothesis that cAMP, acting as an extracellular mediator, affects the physiology and function of human myeloid cells. They found that human monocytes exposed to extracellular cAMP exhibit higher expression of CD14 and lower amount of MHC class I and class II molecules. [J Leukoc Biol] Abstract Expansion and Preferential Activation of the CD14+CD16+ Monocyte Subset during Multiple Sclerosis Little is known about how the two main monocyte subsets, CD14++CD16– and CD14+CD16+, are involved in multiple sclerosis (MS). To understand how the phenotype and responses of these monocyte subsets are altered during MS, total monocytes and the purified monocyte subsets from healthy subjects and MS patients were characterized ex vivo and stimulated in vitro with lipopolysaccharide. [Immunol Cell Biol] Abstract Inverse Associations between Obesity Indicators and Thymic T-Cell Production Levels in Aging Atomic-Bomb Survivors To test the hypothesis that obesity may contribute to the aging of human T-cell immunity, a thousand atomic-bomb survivors were examined for obesity status and ability to produce naive T cells, i.e., T-cell receptor excision circle numbers in CD4 and CD8 T cells. [PLoS One] Full Article Peripheral Blood TIM-3 Positive NK and CD8+ T Cells throughout Pregnancy: TIM-3/Galectin-9 Interaction and Its Possible Role during Pregnancy Researchers investigated the number, phenotype and functional activity of T-cell immunoglobulin and mucin domain (TIM)-3+ peripheral blood mononuclear cells during healthy human pregnancy. [PLoS One] Full Article |