Out-of-Sequence Signal 3 Paralyzes Primary CD4+ T-Cell-Dependent Immunity Scientists showed that during strong stimulation, a profound suppression of primary CD4+ T-cell-mediated immune responses ensued and was observed across preclinical models and patients undergoing high-dose interleukin-2 therapy. These events resulted in complete paralysis of primary CD4+ T cell activation, affecting memory generation and induction of autoimmunity as well as impaired viral clearance. [Immunity] Abstract | Graphical Abstract | Press Release High-Throughput Pairing of T Cell Receptor α and β Sequences Researchers describe and validate a method called pairSEQ, which can leverage the diversity of T cell receptor (TCR) sequences to accurately pair hundreds of thousands of TCR α and TCR β sequences in a single experiment. [Sci Transl Med] Abstract | Press Release Immunosuppressive Human Anti-CD83 Monoclonal Antibody Depletion of Activated Dendritic Cells in Transplantation Investigators developed a human IgG1 monoclonal antibody (mAb), 3C12, specific for CD83, which is expressed on activated but not resting dendritic cells. The 3C12 mAb and an affinity improved version, 3C12C, depleted CD83+ cells by CD16+ NK cell mediated antibody dependent cellular cytotoxicity, and inhibited allogeneic T cell proliferation in vitro. [Leukemia] Abstract Extracellular Signal–Regulated Kinase 5 Promotes Acute Cellular and Systemic Inflammation Researchers inhibited the kinase activities and reduced the abundances of extracellular signal–regulated kinase 5 (ERK5) and MEK5, a mitogen-activated protein kinase kinase directly upstream of ERK5, in primary human vascular endothelial cells and monocytes, and found that ERK5 promoted inflammation induced by a broad range of microbial Toll-like receptor agonists and by the proinflammatory cytokines interleukin-1β and tumor necrosis factor‑α. [Sci Signal] Abstract Activated and Expanded Natural Killer Cells Target Osteosarcoma Tumor Initiating Cells in an NKG2D–NKG2DL Dependent Manner Scientists explored the efficacy and the pathways involved in natural killer (NK) cells’ elimination of osteosarcoma (OS) cells, including tumor initiating cells, which are responsible for chemotherapy resistance, recurrence, and metastasis. They found OS cells were susceptible to activated and expanded NK cells’ lysis both in vivo and in vitro, and this cytolytic activity relied on interaction between NKG2D receptor and NKG2D ligands (NKG2DL). [Cancer Lett] Abstract Age-Related Profiling of DNA Methylation in CD8+ T Cells Reveals Changes in Immune Response and Transcriptional Regulator Genes Researchers studied age-related changes in DNA methylation and gene expression in CD4+ and CD8+ T cells from younger and older individuals. They observed marked difference between T cell subsets, with increased number of methylation changes and higher methylome variation in CD8+ T cells with age. [Sci Rep] Full Article Glycosylation-Dependent Interaction between CD69 and S100A8/S100A9 Complex Is Required for Regulatory T-Cell Differentiation By RNA interference, investigators showed that CD69 is not sufficient to support the conversion of CD4+ naive T cells into regulatory T cells, whereas it does that of human peripheral blood mononuclear cells, suggesting that a ligand-receptor interaction is required for CD69 function. [FASEB J] Abstract TLR3 Drives IRF6-Dependent IL-23p19 Expression and p19/EBI3 Heterodimer Formation in Keratinocytes Investigators report that interferon regulatory factor (IRF) regulates a subset of Toll-like receptor (TLR)3 responses in human keratinocytes, including the production of a novel interleukin-12 (IL-12) family heterodimer (p19/EBI3). They propose that the TLR3-IRF6-p19/EBI3 axis may regulate keratinocyte and/or immune cell functions in the context of cell damage and wound healing in the skin. [Immunol Cell Biol] Full Article Expression of TNFR2 by Regulatory T Cells in Peripheral Blood Is Correlated with Clinical Pathology of Lung Cancer Patients The authors investigated the phenotype and function of tumor necrosis factor receptor type II (TNFR2)+ regulatory T cells (Tregs) present in the peripheral blood of 43 lung cancer patients. Further, the association of TNFR2 expression on Tregs with clinicopathological factors was analyzed. [Cancer Immunol Immunother] Abstract Subscribe to our sister publications: Immunology of Infectious Disease News & Immune Regulation News! |