Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses To characterize the gene expression variation that underlies distinct infection outcomes and monitor infection phenotypes, scientists developed an experimental system that combines single-cell RNA-seq with fluorescent markers. Probing the responses of individual macrophages to invading Salmonella, they found that variation between individual infected host cells is determined by the heterogeneous activity of bacterial factors in individual infecting bacteria. [Cell] Abstract | Graphical Abstract IFN-γ Induction by Neutrophil-Derived IL-17A Homodimer Augments Pulmonary Antibacterial Defense The role of interleukin-17A (IL-17A) in host defense against Legionella pneumophila remains elusive. Researchers found that neutrophils produce IL-17A homodimer during L. pneumophila infection, and hematopoietic cell-derived IL-17A is known to be important for bacterial clearance. [Mucosal Immunol] Abstract CD4+ T-Cell Survival in the GI Tract Requires Dectin-1 during Fungal Infection Scientists showed that Dectin-1 is essential for driving fungal-specific CD4+ T-cell responses in the gastrointestinal (GI) tract. Loss of Dectin-1 resulted in abrogated dendritic cell responses in the mesenteric lymph nodes and defective T-cell co-stimulation, causing substantial increases in CD4+ T-cell apoptosis and reductions in the cellularity of GI-associated lymphoid tissues. [Mucosal Immunol] Full Article Trypanosoma cruzi Infection Imparts a Regulatory Program in Dendritic Cells and T Cells via Galectin-1–Dependent Mechanisms Researchers showed that galectin-1 (Gal-1) functions as a negative regulator to limit host-protective immunity following intradermal infection with T. cruzi. Concomitant with the upregulation of immune inhibitory mediators, including IL-10, TGF-β1, IDO, and programmed death ligand 2, T. cruzi infection induced an early increase of Gal-1 expression in vivo. [J Immunol] Abstract HIV Magnitude and Kinetics of CD8+ T Cell Activation during Hyperacute HIV Infection Impact Viral Set Point Investigators screened high-risk uninfected women twice a week for plasma HIV RNA and identified 12 hyperacute infections. Onset of viremia elicited a massive HIV-specific CD8+ T cell response, with limited bystander activation of non-HIV memory CD8+ T cells. [Immunity] Abstract Sequence-Specific Activation of the DNA Sensor cGAS by Y-Form DNA Structures as Found in Primary HIV-1 cDNA Researchers found that unpaired DNA nucleotides flanking short base-paired DNA stretches, as in stem-loop structures of single-stranded DNA derived from human immunodeficiency virus type 1 (HIV-1), activated the type I interferon–inducing DNA sensor cGAS in a sequence-dependent manner. [Nat Immunol] Abstract | Press Release The Local Environment Orchestrates Mucosal Decidual Macrophage Differentiation and Substantially Inhibits HIV-1 Replication Scientists showed, by using freshly purified decidual macrophages and ex vivo human decidual explants, that the local decidual environment influences decidua basalis differentiation and naturally protects these cells from HIV-1 infection. [Mucosal Immunol] Abstract Impairment of T Cell Development and Acute Inflammatory Response in HIV-1 Tat Transgenic Mice Researchers addressed whether HIV-1 Tat could affect T cell development and acute inflammatory response by generating a transgenic mouse expressing Tat in lymphoid tissue. Tat-Tg mice showed thymus atrophy and the maturation block from DN4 to DP thymic subpopulations, resulting in CD4+ and CD8+ T cells depletion in peripheral blood. [Sci Rep] Full Article Increased Frequency of Circulating Tc22/Th22 Cells and Polyfunctional CD38− T Cells in HIV-Exposed Uninfected Subjects Innate factors like IL-22 exert gut mucosal protection and polyfunctional T cells have been associated with low progression in HIV infection; therefore, investigators evaluated the frequencies of CD4+ and CD8+ T cell-secreting cytokines, including Tc22/Th22 cells and polyfunctional T cells in HIV-1-exposed uninfected individuals, their HIV-1-infected partners and healthy controls. [Sci Rep] Full Article Subscribe to our sister publications: Human Immunology News and Immune Regulation News! |