INTESTINAL CANCERS & DISEASES Inflammasome-Independent Role of AIM2 in Suppressing Colon Tumorigenesis via DNA-PK and Akt Researchers found that absent in melanoma 2 (Aim2)-deficient mice had greater tumor load than Asc-deficient mice in the azoxymethane/dextran sodium sulfate model of colorectal cancer. [Nat Med] Abstract FOXM1-induced PRX3 Regulates Stemness and Survival of Colon Cancer Cells via Maintenance of Mitochondrial Function Researchers used immunohistochemistry and quantitative PCR to compare expression of mitochondrial peroxiredoxin 3 (PRX3) in CD133+CD44+ Lgr5+cells vs CD133–CD44–Lgr5– colon tumor cells. [Gastroenterology] Abstract The Epithelial Polarity Regulator LGALS9/Galectin-9 Induces Fatal Frustrated Autophagy in KRAS Mutant Colon Carcinoma that Depends on Elevated Basal Autophagic Flux The authors uncovered that KRAS mutant colorectal cancer was uniquely sensitive to treatment with recombinant LGALS9/Galectin-9, a recently established regulator of epithelial polarity. [Autophagy] Abstract Voltage-Gated Na+ Channel Activity Increases Colon Cancer Transcriptional Activity and Invasion Via Persistent MAPK Signaling Using pharmacological inhibitors/activators and siRNA-mediated gene knockdowns, scientists correlated channel activity with Rap1-dependent persistent MAPK activation in the SW620 human colon cancer cell line. [Sci Rep] Full Article FBXW7 Negatively Regulates ENO1 Expression and Function in Colorectal Cancer Investigators showed that elevated expression of Enolase 1 (ENO1) was identified in F-box and WD40 domain protein 7 (FBXW7)-depletion HCT116 cells through two-dimensional protein electrophoresis and mass spectrometry assays (2DE-MS). Subsequent western blotting and immunohistochemical assays confirmed that ENO1 expression reversely correlated with FBXW7 expression in several cells and colon cancer tissues. [Lab Invest] Abstract Adult Zebrafish Intestine Resection: A Novel Model of Short Bowel Syndrome, Adaptation, and Intestinal Stem Cell Regeneration Researchers created a new reproducible in vivo model of short bowel syndrome in zebrafish, a tractable vertebrate model, to facilitate investigation of the mechanisms of intestinal adaptation. [Am J Physiol Gastrointest Liver Physiol] Abstract Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in Ulcerative Colitis Investigators showed that the association of IL-10 and glucocorticoids improved the integrity of TNFα-treated Caco-2 cells and that p38 MAPK plays a key role. [PLoS One] Full Article Stimulation of Cell Migration by Flagellin through the p38 MAP Kinase Pathway in Cultured Intestinal Epithelial Cells The authors found that both flagellin and TGF-β1 activated p38 MAPK and its downstream protein kinase, MAPK-activated protein kinase-2 in an IEC-6 intestinal epithelial cell line. [J Cell Biochem] Abstract INTESTINAL STEM CELL & ORGANOID RESEARCH The Sorting Protein PACS-2 Promotes ErbB Signalling by Regulating Recycling of the Metalloproteinase ADAM17 PACS-2 sustained ADAM17 cell-surface activity by diverting ADAM17 away from degradative pathways. Pacs2-deficient mice displayed significantly reduced levels of phosphorylated EGFR and intestinal proliferation. [Nat Commun] Abstract |