Intestinal Cell News Volume 1.23 | Aug 28 2015

Dysregulated CRTC1 Activity Is a Novel Component of PGE2 Signaling that Contributes to Colon Cancer Growth
Researchers provide evidence of enhanced CRTC1 (CREB-regulated transcription co-activator 1) protein content and transcriptional activity in mouse models of sporadic or colitis-associated colon cancer azoxymethane/dextran sulfate sodium, and in human colorectal tumors specimens compared with adjacent normal mucosa. [Oncogene] Abstract

Intestinal-Specific Activatable Myb Initiates Colon Tumorigenesis in Mice
Investigators generated the first intestinal-specific, inducible transgenic model; a MybER transgene encoding a tamoxifen-inducible fusion protein between Myb and the estrogen receptor-α ligand-binding domain driven by the intestinal-specific promoter, Gpa33. [Oncogene] Abstract

DNMT1-Associated Long Non-Coding RNAs Regulate Global Gene Expression and DNA Methylation in Colon Cancer
Researchers tested if long non-coding (lnc)RNAs could associate with DNA methyltransferases to regulate DNA methylation and gene expression. Using RIP-seq, scientists identified a subset of lncRNAs that interact with the DNA methyltransferase DNMT1 in a colon cancer cell line, HCT116. [Hum Mol Genet] Abstract

Enhanced Anti-Colon Cancer Immune Responses with Modified eEF2-Derived Peptides
Scientists indicated that modifications of a segment of peptides from wild type eukaryotic elongation factor-2 (eEF2)-derived immunogenic peptides was able to further enhance its capacity of inducing antigen-specific cytotoxic T lymphocytes against colon cancer cells. [Cancer Lett] Abstract

Hsa-miR-19a Is Associated with Lymph Metastasis and Mediates the TNF-α Induced Epithelial-to-Mesenchymal Transition in Colorectal Cancer
The authors reported that high expression of microRNA-19a (miR-19a) was associated with lymph node metastasis and played an important role in TNF-α-induced epithelial-to-mesenchymal transition in colorectal cancer cells. [Sci Rep] Full Abstract

Glial Cell Line-Derived Neurotrophic Factor (GDNF) Promotes Barrier Maturation and Wound Healing in Intestinal Epithelial Cells In Vitro
Scientists showed that enterocytes represent an additional source of GDNF synthesis. GDNF contributed to wound healing in a cAMP/PKA-dependent manner and promoted barrier maturation in immature enterocytes cells by inactivation of p38MAPK signaling. [Am J Physiol Gastrointest Liver Physiol] Abstract

Loss of Histone Deacetylase Hdac1 Disrupts Metabolic Processes in Intestinal Epithelial Cells
Scientists showed that Hdac1 depletion in cellulo leads to increased histone acetylation after metabolic stresses, and to metabolic disturbances resulting in impaired responses to oxidative stresses, AMPK kinase activation and mitochondrial biogenesis. [FEBS Lett] Abstract

INTESTINAL STEM CELL & ORGANOID RESEARCH

A Mouse Model of Targeted Musashi1 Expression in Whole Intestinal Epithelium Suggests Regulatory Roles in Cell Cycle and Stemness
The authors generated a mouse model, referred to as v-Msi, overexpressing Musashi1 specifically in the entire intestinal epithelium. Compared with wild type litters, v-Msi1 mice exhibited increased intestinal crypt size accompanied by enhanced proliferation. Comparative transcriptomics by RNA-seq revealed Musashi1’s association with gut stem cell signature, cell cycle, DNA replication and drug metabolism. [Stem Cells] Abstract

Ttk69 Acts as a Master Repressor of Enteroendocrine Cell Specification in Drosophila Intestinal Stem Cell Lineages
Scientists identified Ttk69, a BTB domain-containing transcriptional repressor, as a master repressor of enteroendocrine cell specification in the intestinal stem cell lineages. [Development] Abstract

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RedHill Biopharma Receives Notice of Allowance for New Israeli Patent Covering RHB-104 for the Treatment of Crohn’s Disease
RedHill Biopharma Ltd. announced that it has received a Notice of Allowance from the Israeli Patent Office for a new Israeli patent No. 207420 covering the formulation of RHB-104, a proprietary and potentially groundbreaking antibiotic combination therapy in oral capsule formulation, with potent intracellular, anti-mycobacterial and anti-inflammatory properties. [RedHill Biopharma Ltd.] Press Release

Immatics and MD Anderson Announce Launch of Immatics US, Inc., to Develop Multiple T-Cell and TCR-Based Adoptive Cellular Therapies
Immatics Biotechnologies GmbH and The University of Texas MD Anderson Cancer Center announced the launch of Immatics US, Inc., a new company aiming at becoming a global leader in adoptive cellular therapies for the treatment of a range of tumor types. [The University of Texas MD Anderson Cancer Center] Press Release

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Research Associate – Intestinal Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Postdoctoral or PhD position – Intestinal Lipidomics (Max Planck Institute of Molecular Cell Biology and Genetics)

Senior Research Technician (Qu Biologics Inc.)

Genetic Counselor – Cancer (Fred Hutchinson Cancer Research Center)

Postdoctoral Fellow – Biotechnology (Qu Biologics Inc.)

Harry Eagle Scholar Awards for Postdoctoral Candidates (Albert Einstein College of Medicine of Yeshiva University)

Postdoctoral Research Fellow – Ex Vivo Tumor Organoid Models (Fred Hutchinson Cancer Research Center)

Postdoctoral Positions – Cancer Research (Rutgers University)

Postdoctoral Researcher – Immunology and Microbiology (New York University School of Medicine)

Postdoctoral Position – Intestinal Immunology (Shanghai Institute of Immunology)

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