INTESTINAL CANCERS & DISEASES RNA Binding Protein RBM3 Increases β-Catenin Signaling to Increase Stem Cell Characteristics in Colorectal Cancer Cells Researchers investigated the role of the RNA binding protein RBM3 in stem cell homeostasis in colorectal cancers. Using a doxycycline inducible RBM3 overexpressing cell lines HCT 116 and DLD-1, they measured changes in side population cells that have high xenobiotic efflux capacity and increased capacity for self-renewal. [Mol Carcinog] Abstract “Store-Operated” cAMP Signaling Contributes to Ca2+-Activated Cl– Secretion in T84 Colonic Cells Investigators assessed the degree to which store-operated cAMP signaling participates in Ca2+-activated Cl– transport as measured by transepithelial short-circuit current in polarized T84 monolayers in parallel with imaging of cAMP and PKA activity using FRET-based reporters in single cells. [Am J Physiol Gastrointest Liver Physiol] Abstract Epibrassinolide Alters PI3K/MAPK Signaling Axis via Activating Foxo3a-Induced Mitochondria-Mediated Apoptosis in Colon Cancer Cells Researchers investigated the potential apoptotic effect of epibrassinolide (EBR) on stress-related and survival signaling molecules in colon carcinoma cells. EBR decreased cell viability and colony formation in HCT 116 and HT-29 colon carcinoma cells. [Exp Cell Res] Abstract 3-O-(2′E,4′Z-Decadienoyl)-20-O-Acetylingenol Induces Apoptosis in Intestinal Epithelial Cells of Rats via Mitochondrial Pathway The authors elucidated the cytotoxic mechanism of 3-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol in intestinal epithelial cells of rats to guide safer application of this herb in the clinic. [J Ethnopharmacol] Abstract Uptake of Gold Nanoparticles by Intestinal Epithelial Cells: Impact of Particle Size on Their Absorption, Accumulation, and Toxicity Scientists determined the influence of particle size and concentration of gold nanoparticles (AuNPs) on their absorption, accumulation, and cytotoxicity in model intestinal epithelial cells. [J Agric Food Chem] Abstract | Graphical Abstract Bisphenol A at the Reference Level Counteracts Doxorubicin Transcriptional Effects on Cancer Related Genes in HT29 Cells Researchers aimed to assess potential interactions between bisphenol A (BPA) and doxorubicin (DOX) on HT29 colon cancer cells. HT29 cell response was evaluated after exposure to BPA, DOX, or co-exposure to both chemicals. [Toxicol In Vitro] Abstract Down-Regulation of MicroRNA-381 Promotes Cell Proliferation and Invasion in Colon Cancer through Up-Regulation of LRH-1 The authors found that microRNA (miR)-381 was significantly down-regulated in human colon cancer tissues, compared with adjacent normal tissues. Introduction of miR-381 antisense oligos into SW480 and HCT116 cells promoted cell proliferation and invasion. [Biomed Pharmacother] Abstract INTESTINAL STEM CELL & ORGANOID RESEARCH Efficient Genetic Engineering of Human Intestinal Organoids Using Electroporation Scientists describe a detailed protocol for the generation of intestinal organoids by culture with essential growth factors in a basement membrane matrix. [Nat Protoc] Full Article Bunched and Madm Function Downstream of Tuberous Sclerosis Complex to Regulate the Growth of Intestinal Stem Cells in Drosophila Investigators showed that the leucine zipper protein Bunched and the adaptor protein Madm are novel regulators of intestinal stem cells. [Stem Cell Rev] Full Article |