INTESTINAL CANCERS & DISEASES Tumor-Promoting Role of SOCS1 in Colorectal Cancer Cells Researchers investigated SOCS1 relevance for colorectal cancer (CRC) by querying gene expression datasets of human CRC specimens from The Cancer Genome Atlas, and by SOCS1 gain/loss-of-function analyses in murine and human colon carcinoma cells. [Sci Rep] Full Article REG4 Is a Transcriptional Target of GATA6 and Is Essential for Colorectal Tumorigenesis Scientists showed that regenerating islet-derived (REG)4, a member of the regenerating islet-derived family, is also a target of GATA6. They further demonstrated that REG4 is downregulated by overexpression of miR-363, which suppresses GATA6 expression. [Sci Rep] Full Article Interleukin-13 Affects the Epithelial Sodium Channel in the Intestine by Coordinated Modulation of STAT6 and p38 MAPK Activity Scientists investigated whether interleukin-13 (IL-13) also acts as a potent modulator of epithelial sodium transport via epithelial sodium channel (ENaC), and the signaling components involved. The effect of IL-13 on ENaC was examined in HT-29/B6-GR/MR human colon cells, as well as in mouse distal colon, by measuring amiloride-sensitive short-circuit current in Ussing chambers. [J Physiol] Abstract GSK3β and Gli3 Play a Role in Activation of Hedgehog-Gli Pathway in Human Colon Cancer – Targeting GSK3β Downregulates the Signaling Pathway and Reduces Cell Proliferation Investigators showed that inhibition of GSK3β with lithium chloride enhances Gli3 processing into its repressor form, consequently downregulating Hedgehog-Gli signaling, reducing cell proliferation and inducing cell death. [Biochim Biophys Acta] Abstract Flavaglines Ameliorate Experimental Colitis and Protect Against Intestinal Epithelial Cell Apoptosis and Mitochondrial Dysfunction Researchers tested the therapeutic potential of flavaglines to promote intestinal epithelial cell homeostasis and to protect against a model of experimental colitis in which inflammation is driven by epithelial ulceration. [Inflamm Bowel Dis] Abstract PI3K/Akt/mTOR Pathway Dual Inhibitor BEZ235 Suppresses the Stemness of Colon Cancer Stem Cells Investigators isolated colon cancer stem cells through sphere culture and verified with the cancer stem cell markers CD133, CD44, and CD24. They demonstrated that the PI3K/Akt/mTOR signaling pathway was highly activated in the colon cancer stem cells and that inhibition of the PI3K/Akt/mTOR pathway by the inhibitor BEZ235 suppressed the colon cancer stem cell proliferation with reduced stemness indicated by CD133 and Lgr5 expressions. [Clin Exp Pharmacol Physiol] Abstract Inhibition of Migration and Induction of Apoptosis in LoVo Human Colon Cancer Cells by Polysaccharides from Ganoderma Lucidum Scientists evaluated the anticancer effects and investigated the underlying molecular mechanisms of ganoderma lucidum polysaccharides (GLPs) on LoVo human colon cancer cells. They demonstrated that the GLP-mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. [Mol Med Rep] Abstract INTESTINAL STEM CELL & ORGANOID RESEARCH Type 3 Innate Lymphoid Cells Maintain Intestinal Epithelial Stem Cells after Tissue Damage Using type 3 innate lymphoid cells (ILC3s)-deficient Lgr5 reporter mice, researchers showed that maintenance of intestinal stem cells after damage is severely impaired in the absence of ILC3s or the ILC3 signature cytokine IL-22. [J Exp Med] Abstract Intestinal Stem Cell Growth and Differentiation on a Tubular Scaffold with Evaluation in Small and Large Animals The authors investigated the growth and differentiation of intestinal stem cells on a novel tubular scaffold in vitro and in vivo. [Regen Med] Abstract |