Intestinal Cell News Volume 1.39 | Dec 18 2015

Contributions of Microbiome and Mechanical Deformation to Intestinal Bacterial Overgrowth and Inflammation in a Human Gut-on-a-Chip
A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. [Proc Natl Acad Sci USA] Abstract | Press Release

Ubiquitin-Specific Protease 4-Mediated Deubiquitination and Stabilization of PRL-3 Is Required for Potentiating Colorectal Oncogenesis
Scientists demonstrated that aberrant expression of ubiquitin specific protease 4 (USP4) contributes to the development and progression of colorectal cancer and revealed a critical mechanism underlying USP4-mediated oncogenic activity. [Cancer Res] Abstract

The Msi Family of RNA-Binding Proteins Function Redundantly as Intestinal Oncoproteins
Investigators demonstrated that MSI1 and MSI2 act as functionally redundant oncoproteins required for the ontogeny of intestinal cancers. [Cell Rep] Full Article | Graphical Abstract

Combination of Tolfenamic Acid and Curcumin Induces Colon Cancer Cell Growth Inhibition by Modulating Reactive Oxygen Species and Specific Transcription Factors
Researchers investigated the therapeutic efficacy of curcumin in combination with the small molecule tolfenamic acid in colorectal cancer cell lines. [Oncotarget] Full Article

Network-Level Effects of Kinase Inhibitors Modulate TNF-α-Induced Apoptosis in the Intestinal Epithelium
Researchers found that inhibitors of the mitogen-activated protein kinase kinase kinase Raf, MEK, or extracellular signal-regulated kinase exerted distinct effects on the timing and magnitude of tumor necrosis factor-α (TNF-α)-induced apoptosis in the mouse intestine. [Sci Signal] Abstract

Treating Colon Cancer Cells with FK228 Reveals a Link between Histone Lysine Acetylation and Extensive Changes in the Cellular Proteome
The authors used stable isotope labeling by amino acids in cell culture and affinity enrichment followed by high-resolution liquid chromatograph-mass spectrometer (LC-MS)/MS analysis to quantitate the changes in the lysine acetylome in HCT-8 cells after FK228 treatment. [Sci Rep] Full Article

A Simple Coculture System Shows Mutualism between Anaerobic Faecalibacteria and Epithelial Caco-2 Cells
Researchers describe the development of a coculture system for intestinal Caco-2 cells and an anaerobic symbiont, Faecalibacterium prausnitzii. [Sci Rep] Full Article

INTESTINAL STEM CELL & ORGANOID RESEARCH

HOXA5 Counteracts Stem Cell Traits by Inhibiting Wnt Signaling in Colorectal Cancer
Researchers identified HOXA5 as an important repressor of intestinal stem cell fate in vivo and identified a reciprocal feedback between HOXA5 and Wnt signaling. [Cancer Cell]
Abstract | Graphical Abstract | Press Release

Hindsight/RREB-1 Functions in Both the Specification and Differentiation of Stem Cells in the Adult Midgut of Drosophila
Investigators showed that hindsight is required for enterocyte (EC) differentiation in the context of intestinal stem cells-to-enteroblast differentiation, but not in the context of adult midgut precursor-to-EC differentiation. [Biol Open] Full Article

Visit our reviews page to see a complete list of reviews in the intestinal cell research field.

Galapagos and Gilead Announce Global Partnership to Develop Filgotinib for the Treatment of Rheumatoid Arthritis and Other Inflammatory Diseases
Galapagos NV and Gilead Sciences, Inc. announced that the companies have entered into a global partnership for the development and commercialization of the JAK1-selective inhibitor filgotinib for inflammatory disease indications. [Galapagos NV] Press Release

From our sponsor: Learn about applications of ALDHbr cells in regenerative medicine. Watch the video.

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

Visit our events page to see a complete list of events in the intestinal cell research community.

PhD Student – Colorectal/Pancreatic Cancer (German Cancer Research Center)

Postdoctoral Fellow – Cancer Immunotherapy (Inserm)

Postdoctoral Scientist – Gastroenterology and Immunology (University of Oxford)

Research Associate – Immunology and Inflammation (Imperial College London)

Research Technician – Preclinical and Translational Research (Qu Biologics Inc.)

Faculty Position – Autoimmune Disease and Rheumatologic Inflammation (Cleveland Clinic)

Computational Postdoctoral Associate – Inflammatory Bowel Disease (Icahn School of Medicine at Mount Sinai)

Postdoctoral Associate – Epigenetics of Inflammatory Bowel Disease (Icahn School of Medicine at Mount Sinai)

Open Group Leader Position – Cancer Research (Oslo University Hospital)

Postdoctoral Research Fellow – Ex Vivo Tumor Organoid Models (Fred Hutchinson Cancer Research Center)

Recruit Top Talent: Reach more than 60,000 potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.