INTESTINAL CANCERS & DISEASES A Long Noncoding RNA Associated with Susceptibility to Celiac Disease The authors report on the identification and characterization of a long noncoding RNA (lncRNA), lnc13, that harbors a celiac disease-associated haplotype block and represses expression of certain inflammatory genes under homeostatic conditions. [Science] Abstract Differential Clonal Evolution in Oesophageal Cancers in Response to Neo-Adjuvant Chemotherapy Scientists report whole-exome and deep sequencing of 30 paired oesophageal adenocarcinomas sampled before and after neo-adjuvant chemotherapy. [Nat Commun] Full Article SETD7 Controls Intestinal Regeneration and Tumorigenesis by Regulating Wnt/β-Catenin and Hippo/YAP Signaling Researchers showed that SETD7 is required for Wnt-driven intestinal tumorigenesis and regeneration. Mechanistically, SETD7 is part of a complex containing Hippo/Yes-Associated Protein (YAP), AXIN1, and β-catenin, and SETD7-dependent methylation of YAP facilitates Wnt-induced nuclear accumulation of β-catenin. [Dev Cell] Abstract Epigenetic Silencing of miR-124 Prevents Spermine Oxidase Regulation: Implications for Helicobacter Pylori-Induced Gastric Cancer The polyamine catabolic enzyme spermine oxidase (SMOX) is induced in chronic inflammatory conditions, including Helicobacter pylori-associated gastritis, where its production of hydrogen peroxide contributes to DNA damage and subsequent tumorigenesis. The authors sought to determine if repression of miR-124 is associated with elevated SMOX activity and concluded that miR-124 is indeed a negative regulator of SMOX. [Oncogene] Abstract Sphingosine-1-Phosphate Receptor-1 (S1P) Is Expressed by Lymphocytes, Dendritic Cells, and Endothelium and Modulated during Inflammatory Bowel Disease The authors examined cell subsets that express sphingosine-1-phosphate receptor-1 (S1P1) in the intestine using S1P1-eGFP mice, the regulation of S1P1 expression in lymphocytes after administration of dextran sulfate sodium, after colitis induced by transfer of CD4+CD45RBhi cells, and by crossing a mouse with TNF-driven ileitis with S1P1-eGFP mice. [Mucosal Immunol] Abstract The Caspase-1 Inhibitor AC-YVAD-CMK Attenuates Acute Gastric Injury in Mice: Involvement of Silencing NLRP3 Inflammasome Activities Researchers evaluated the protective effects of inhibiting caspase-1 activity or gastric acid secretion on acute gastric injury in mice. [Sci Rep] Full Article Targeting HCCR Expression Resensitizes Gastric Cancer Cells to Chemotherapy via Down-Regulating the Activation of STAT3 Scientists evaluated human cervical cancer oncogene (HCCR) expression in several gastric cancer cell lines and in one normal gastric mucosal cell line. [Sci Rep] Full Article Phospholipase Cϵ Activates Nuclear Factor-κB Signaling by Causing Cytoplasmic Localization of Ribosomal S6 Kinase and Facilitating Its Phosphorylation of Inhibitor κB in Colon Epithelial Cells The authors showed, by employing the colitis-induced colorectal carcinogenesis model, where APCMin/+ mice are administered with dextran sulfate sodium, that phospholipase Cϵ knockout alleviates colitis and suppresses the following tumorigenesis concomitant with marked attenuation of proinflammatory cytokine expression. [J Biol Chem] Abstract | Full Article Physiological Concentration of Exogenous Lactate Reduces Antimycin a Triggered Oxidative Stress in Intestinal Epithelial Cell Line IPEC-1 and IPEC-J2 In Vitro Researchers hypothesized that both lactobacilli and lactate influence porcine intestinal epithelial cells. In vivo experiments showed that concentration of lactate was significantly higher in gastric, duodenal and jejunal chyme of suckling piglets compared to their weaned counterparts. [PLoS One] Full Article R-Spondins Are Expressed by the Intestinal Stroma and Are Differentially Regulated during Citrobacter Rodentium- and DSS-Induced Colitis in Mice RNA sequencing and qRT-PCR were used to assess the expression of R-spondins at steady state and in two mouse models of colonic inflammation. The cellular source of R-spondins was assessed in specific colonic cell populations isolated by cell sorting. [PLoS One] Full Article INTESTINAL STEM CELL & ORGANOID RESEARCH Epidermal Growth Factor Suppresses Intestinal Epithelial Cell Shedding via a MAPK Dependent Pathway. Investigators asked whether shedding is controlled by epidermal growth factor receptor, an important driver of intestinal growth and differentiation. [J Cell Sci] Abstract | Press Release |