Intestinal Cell News Volume 3.38 | Oct 6 2017

TOP STORY

Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo

The authors investigated the impact of infection with the enteric pathogen C. rodentium on mouse intestinal epithelial cell metabolism using global proteomic and targeted metabolomics and lipidomics. The major signatures of the infection were upregulation of the sugar transporter Sglt4, aerobic glycolysis, and production of phosphocreatine, which mobilizes cytosolic energy. [Cell Metab] Full Article | Graphical Abstract

(Ranked by impact factor of the journal)

Synthesis and Characterization of Well-Defined Hydrogel Matrices and Their Application to Intestinal Stem Cell and Organoid Culture

Researchers describe a protocol for the generation of well-defined matrices for the culture of intestinal stem cells (ISCs) and intestinal organoids. These matrices comprise a poly(ethylene glycol) hydrogel backbone functionalized with minimal adhesion cues including RGD, which is sufficient for ISC expansion, and laminin-111, which is required for organoid formation. [Nat Protoc] Abstract

A Molecularly Annotated Model of Patient-Derived Colon Cancer Stem-Like Cells to Assess Genetic and Non-Genetic Mechanisms of Resistance to Anti-EGFR Therapy

Xenospheres faithfully retained the genetic make-up of their matched xenopatients over in vitro and in vivo passages. Frequent and rare genetic lesions triggering primary resistance to cetuximab through constitutive activation of the RAS signaling pathway were conserved, as well as the vulnerability to their respective targeted treatments. [Clin Cancer Res] Abstract

A Novel Mechanism of lncRNA and miRNA Interaction: CCAT2 Regulates miR-145 Expression by Suppressing Its Maturation Process in Colon Cancer Cells

Investigators have observed that modulated expression of colon cancer-associated transcript-2 (CCAT2) regulates the expression of miR-145 in colon cancer HCT-116 and HT-29 cells. Knockout of CCAT2 increases miR-145 and negatively regulates miR-21 in HCT-116 cells, impairs proliferation and differentiation. [Mol Cancer] Full Article

A Novel Acridine Derivative, LS-1-10 Inhibits Autophagic Degradation and Triggers Apoptosis in Colon Cancer Cells

Scientists found that 9-chloro-2-(3-(dimethylamino)propyl)pyrrolo[2,3,4-kl]acridin-1(2H)-one (LS-1-10) was the most effective from a library at inhibiting autophagic-mediated degradation and could decrease the viability of multiple colon cancer cells. [Cell Death Dis] Full Article

Human Intestinal Organoids Express Histo-Blood Group Antigens, Bind Norovirus VLPs, and Support Limited Norovirus Replication

The authors found that fully developed human intestinal organoids (HIOs) express effectively various types 1 and 2 HBGAs, including Lewis, secretor, and nonsecretor antigens, distributing on the glycocalyx. Selected huNoV-like particles (VLPs) bound the glycocalyx of HIOs with matched HBGA phenotypes. [Sci Rep] Full Article

Combination Curcumin and (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Carcinoma Microenvironment-Induced Angiogenesis by JAK/STAT3/IL-8 Pathway

Investigators simulated the colorectal carcinoma microenvironment by conditioned medium of colorectal carcinoma cell lines supernatant or colorectal carcinoma tissue homogenate supernatant to induce normal endothelial cells. [Oncogenesis] Full Article

A New Clerodane Furano Diterpene Glycoside from Tinospora cordifolia Triggers Autophagy and Apoptosis in HCT-116 Colon Cancer Cells

The in vitro anti-cancer activity of TC-2 was evaluated by SRB assay and the autophagic activity was investigated by immunofluorescence microscopy. Annexin-V FITC and PI dual staining was applied for the detection of apoptosis. [J Ethnopharmacol] Abstract

Optical Clearing of Small Intestine for Three-Dimensional Visualization of Cellular Proliferation within Crypts

Researchers demonstrated successful clearing of jejunal samples with readily visible EdU staining by means of confocal microscopy. Three-dimensional reconstruction of labeled samples reveals preservation of intestinal cytoarchitecture including muscular, submucosal, and mucosal layers. [J Anat] Abstract

Safety and Efficacy of Nintedanib for the Treatment of Metastatic Colorectal Cancer

The authors summarize the pharmacology, efficacy and safety profile of nintedanib in the context of metastatic colorectal cancer, and provide some perspective regarding the role of this drug in clinical practice. [Expert Opin Investig Drugs] Abstract

Visit our reviews page to see a complete list of reviews in the intestinal cell research field.

RedHill Biopharma Announces Positive Top-Line Results from Phase II Study of BEKINDA in Patients with IBS-D

RedHill Biopharma Ltd. announced positive top-line results from the Phase II clinical study of BEKINDA^® 12 mg for the treatment of diarrhea-predominant irritable bowel syndrome. [RedHill Biopharma Ltd.] Press Release

Seres Therapeutics Reports Positive Topline Results from SER-287 Phase Ib Study in Patients with Ulcerative Colitis

Seres Therapeutics, Inc. announced positive topline results from a SER-287 Phase Ib placebo-controlled induction study in 58 patients with mild-to-moderate Ulcerative Colitis, who were failing current therapies. [Seres Therapeutics, Inc.] Press Release

Navajo Nation Reconsiders Ban on Genetic Research

When the Navajo Nation opens its first oncology centre next year in Tuba City, Arizona, clinicians there may be able to offer a service that has been banned on tribal lands for 15 years: analyzing the DNA of Navajo tribe members to guide treatments and study the genetic roots of disease. [Nature News] Editorial

Does Your State Get Its Fair Share of Federal Research Dollars?

The only physicist in Congress has introduced a bill (H.R. 3763) that could rekindle a debate over how to deal with geographic disparities in the allocation of federal research funding. [ScienceInsider] Editorial

Scientists Plead with Brazilian Government to Restore Funding

Anxiety is growing in Brazil over the country’s collapsing research budgets. President Michel Temer had slashed funding for science by 44% in March and has proposed additional decreases for 2018 — even as some science institutes run out of money for basic needs, such as paying electricity bills. The 2017 science budget, at 3.2 billion reais (US$1 billion), is the lowest the country has seen in at least 12 years. [Nature News] Editorial

Judge Recommends Ruling to Block Internet Access to Sci-Hub

The American Chemical Society (ACS) brought a case against Sci-Hub, a pirate site providing access to scientific articles, for copyright and trademark infringement. Following a hearing at a court in Virginia, Magistrate Judge John Anderson filed a report that recommended ruling in favor of ACS on all counts. [The Scientist] Editorial

NEW 14^th Annual Stem Cell Symposium: Technology Innovation for Stem Cell Research and Therapy
February 2, 2018
Los Angeles, California, United States

Visit our events page to see a complete list of events in the community.

NEW Faculty Positions – Cancer Research (Huntsman Cancer Institute)

Research Technologist – Intestinal Stem Cell Biology (STEMCELL Technologies Inc.)

Postdoctoral Fellow – Cell Biology, Physiology, and Molecular Genetics (Children’s Hospital of Philadelphia)

Research Associate – Mechanical Cell Competition (University of Bristol)

Faculty Position – Molecular Oncology (Moffitt Cancer Center)

Postdoctoral Positions – Stem Cell and Cancer Biology (Johns Hopkins University)

Scientist – Colorectal Cancer (Italian Cancer Research Association)

Faculty Positions – Basic and Translational Cancer Research (Texas Tech University Health Sciences Center El Paso)

Researcher/Faculty Member – Cancer Biology (University of Cincinnati)

Postdoctoral Opportunities – Biology of Colorectal Cancer (Albert Einstein College of Medicine)

Postdoctoral Fellow – Gastric Stem Cells (Harvard University)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

Have we missed an important article or publication in Intestinal Cell News? Click here to submit!

Comments or suggestions? Submit your feedback here.