Intestinal Cell News 3.38 October 6, 2017 | |
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TOP STORYThe authors investigated the impact of infection with the enteric pathogen C. rodentium on mouse intestinal epithelial cell metabolism using global proteomic and targeted metabolomics and lipidomics. The major signatures of the infection were upregulation of the sugar transporter Sglt4, aerobic glycolysis, and production of phosphocreatine, which mobilizes cytosolic energy. [Cell Metab] Full Article | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Researchers describe a protocol for the generation of well-defined matrices for the culture of intestinal stem cells (ISCs) and intestinal organoids. These matrices comprise a poly(ethylene glycol) hydrogel backbone functionalized with minimal adhesion cues including RGD, which is sufficient for ISC expansion, and laminin-111, which is required for organoid formation. [Nat Protoc] Abstract Xenospheres faithfully retained the genetic make-up of their matched xenopatients over in vitro and in vivo passages. Frequent and rare genetic lesions triggering primary resistance to cetuximab through constitutive activation of the RAS signaling pathway were conserved, as well as the vulnerability to their respective targeted treatments. [Clin Cancer Res] Abstract Investigators have observed that modulated expression of colon cancer-associated transcript-2 (CCAT2) regulates the expression of miR-145 in colon cancer HCT-116 and HT-29 cells. Knockout of CCAT2 increases miR-145 and negatively regulates miR-21 in HCT-116 cells, impairs proliferation and differentiation. [Mol Cancer] Full Article Scientists found that 9-chloro-2-(3-(dimethylamino)propyl)pyrrolo[2,3,4-kl]acridin-1(2H)-one (LS-1-10) was the most effective from a library at inhibiting autophagic-mediated degradation and could decrease the viability of multiple colon cancer cells. [Cell Death Dis] Full Article The authors found that fully developed human intestinal organoids (HIOs) express effectively various types 1 and 2 HBGAs, including Lewis, secretor, and nonsecretor antigens, distributing on the glycocalyx. Selected huNoV-like particles (VLPs) bound the glycocalyx of HIOs with matched HBGA phenotypes. [Sci Rep] Full Article Investigators simulated the colorectal carcinoma microenvironment by conditioned medium of colorectal carcinoma cell lines supernatant or colorectal carcinoma tissue homogenate supernatant to induce normal endothelial cells. [Oncogenesis] Full Article The in vitro anti-cancer activity of TC-2 was evaluated by SRB assay and the autophagic activity was investigated by immunofluorescence microscopy. Annexin-V FITC and PI dual staining was applied for the detection of apoptosis. [J Ethnopharmacol] Abstract Researchers demonstrated successful clearing of jejunal samples with readily visible EdU staining by means of confocal microscopy. Three-dimensional reconstruction of labeled samples reveals preservation of intestinal cytoarchitecture including muscular, submucosal, and mucosal layers. [J Anat] Abstract | |
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REVIEWSSafety and Efficacy of Nintedanib for the Treatment of Metastatic Colorectal Cancer The authors summarize the pharmacology, efficacy and safety profile of nintedanib in the context of metastatic colorectal cancer, and provide some perspective regarding the role of this drug in clinical practice. [Expert Opin Investig Drugs] Abstract Visit our reviews page to see a complete list of reviews in the intestinal cell research field. | |
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INDUSTRY NEWSRedHill Biopharma Ltd. announced positive top-line results from the Phase II clinical study of BEKINDA® 12 mg for the treatment of diarrhea-predominant irritable bowel syndrome. [RedHill Biopharma Ltd.] Press Release Seres Therapeutics, Inc. announced positive topline results from a SER-287 Phase Ib placebo-controlled induction study in 58 patients with mild-to-moderate Ulcerative Colitis, who were failing current therapies. [Seres Therapeutics, Inc.] Press Release | |
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POLICY NEWSNavajo Nation Reconsiders Ban on Genetic Research When the Navajo Nation opens its first oncology centre next year in Tuba City, Arizona, clinicians there may be able to offer a service that has been banned on tribal lands for 15 years: analyzing the DNA of Navajo tribe members to guide treatments and study the genetic roots of disease. [Nature News] Editorial Does Your State Get Its Fair Share of Federal Research Dollars? The only physicist in Congress has introduced a bill (H.R. 3763) that could rekindle a debate over how to deal with geographic disparities in the allocation of federal research funding. [ScienceInsider] Editorial Scientists Plead with Brazilian Government to Restore Funding Anxiety is growing in Brazil over the country’s collapsing research budgets. President Michel Temer had slashed funding for science by 44% in March and has proposed additional decreases for 2018 — even as some science institutes run out of money for basic needs, such as paying electricity bills. The 2017 science budget, at 3.2 billion reais (US$1 billion), is the lowest the country has seen in at least 12 years. [Nature News] Editorial Judge Recommends Ruling to Block Internet Access to Sci-Hub The American Chemical Society (ACS) brought a case against Sci-Hub, a pirate site providing access to scientific articles, for copyright and trademark infringement. Following a hearing at a court in Virginia, Magistrate Judge John Anderson filed a report that recommended ruling in favor of ACS on all counts. [The Scientist] Editorial
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EVENTSNEW 14th Annual Stem Cell Symposium: Technology Innovation for Stem Cell Research and Therapy Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Faculty Positions – Cancer Research (Huntsman Cancer Institute) Research Technologist – Intestinal Stem Cell Biology (STEMCELL Technologies Inc.) Research Associate – Mechanical Cell Competition (University of Bristol) Faculty Position – Molecular Oncology (Moffitt Cancer Center) Postdoctoral Positions – Stem Cell and Cancer Biology (Johns Hopkins University) Scientist – Colorectal Cancer (Italian Cancer Research Association) Researcher/Faculty Member – Cancer Biology (University of Cincinnati) Postdoctoral Opportunities – Biology of Colorectal Cancer (Albert Einstein College of Medicine) Postdoctoral Fellow – Gastric Stem Cells (Harvard University) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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