Intestinal Cell News Volume 4.01 | Jan 12 2018

TOP STORY

A Surgical Orthotopic Organoid Transplantation Approach in Mice to Visualize and Study Colorectal Cancer Progression

To study tumor progression in the natural in vivo environment, the authors developed an orthotopic approach based on transplantation of colorectal cancer organoids into the cecal epithelium. The 20 minute procedure is described in detail here and enables growth of transplanted organoids into a single tumor mass within the intestinal tract. [Nat Protoc] Abstract

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BRAF Inhibition Upregulates a Variety of Receptor Tyrosine Kinases and Their Downstream Effector Gab2 in Colorectal Cancer Cell Lines

Scientists showed that BRAF inhibition leads to the upregulation of a variety of receptor tyrosine kinases in colorectal cancer cell lines, including not only the epidermal growth factor receptor, but also human epidermal growth factor receptor (HER) 2 and HER3. [Oncogene] Abstract

Effects of Metformin on Colorectal Cancer Stem Cells Depend on Alterations in Glutamine Metabolism

Metformin induced pAMPK activation and pS6 suppression in metformin-sensitive (HT29) cells, but not in metformin-resistant (SW620) cells. The oxygen consumption rate was higher in HT29 cells than in SW620 cells and showed a prominent decrease after metformin treatment in HT29 cells. [Sci Rep] Full Article

MHC Class I Expression in Intestinal Cells Is Reduced by Rotavirus Infection and Increased in Bystander Cells Lacking Rotavirus Antigen

Researchers assessed class I major histocompatibility complex (MHCI) expression in HT-29 intestinal epithelial cells following rotavirus infection. MHCI levels were upregulated in a partially type I interferon-dependent manner in bystander cells lacking rotavirus antigen, but not in infected cells. [Sci Rep] Full Article

Emodin Inhibits Colon Cancer Cell Invasion and Migration by Suppressing Epithelial-Mesenchymal Transition via the Wnt/β-Catenin Pathway

Investigators found that emodin inhibited the invasion and migration ability of RKO cells and decreased the expression of matrix metalloproteinase-7 (MMP-7), MMP-9, and vascular endothelial growth factor in a dose dependent manner. [Oncol Res] Full Article

Effect of Long Non-Coding RNA Gas5 on Proliferation, Migration, Invasion and Apoptosis of Colorectal Cancer HT-29 Cell Line

Gas5 expression was lower in colorectal cancer (CRC) tissues compared to the adjacent normal tissues, and was also lower in CRC cell lines than FHC cell line. [Cancer Cell Int] Full Article

Aspirin Inhibited the Metastasis of Colon Cancer Cells by Inhibiting the Expression of Toll-Like Receptor 4

The authors demonstrated that lipopolysaccharides could enhance the metastasis potential of C26 and HCT116 colon cancer cell lines. However, aspirin effectively decreased the metastasis capacity of colon cancer cells in vitro and in vivo. [Cell Biosci] Full Article

miR-3120-5p Promotes Colon Cancer Stem Cell Stemness and Invasiveness through Targeting Axin2

Researchers used colon cancer cell lines HCT-116 and SW-480 to investigated the effects of miR-3120-5p on stemness and invasiveness of colon cancer. They found that the population of CD133⁺ and Lgr5⁺ stem cells in both cell lines expressed miR-3120-5p highly, and introducing miR-3120-5p into both cell lines increased the population of cancer stem cells, as measured by flow cytometry, qRT-PCR and sphere formation assays. [Biochem Biophys Res Commun] Abstract

CXCL12 Gene Silencing Down-Regulates Metastatic Potential via Blockage of MAPK/PI3K/AP-1 Signaling Pathway in Colon Cancer

The authors investigated the effect of CXCL12 gene silencing on proliferation,invasion, angiogenesis and the relationship of MAPK/PI3K/AP-1 signaling pathway in colon cancer cells. [Clin Transl Oncol] Full Article

Wnt, RSPO and Hippo Signaling in the Intestine and Intestinal Stem Cells

The authors address aspects of Wnt, R-Spondin (RSPO) and Hippo signaling, in both healthy and transformed intestinal epithelium. In intestinal stem cells, the Wnt pathway is essential for intestinal crypt formation and renewal, whereas RSPO-mediated signaling mainly affects ISC numbers. [Genes (Basel)] Abstract

Visit our reviews page to see a complete list of reviews in the intestinal cell research field.

STEMCELL Technologies Brings Groundbreaking Intestinal and Brain Organoid Culture Tools to the Global Research Community

STEMCELL Technologies Inc. has released two product lines for organoid research that will enable scientists to create powerful models for studying human disease in the lab. [STEMCELL Technologies Inc.] Press Release

IntegraGen and GoPath Laboratories Sign Licensing Agreement for the Commercialization of miR-31-3p Test in North America

IntegraGen and GoPath Laboratories announced the signing of a non-exclusive licensing agreement allowing GoPath to develop and provide a test based on IntegraGen’s proprietary miR-31-3p biomarker to physicians in the United States and Canada. [IntegraGen (Business Wire, Inc.)] Press Release

Rising Star Appointed UK Science Minister

The United Kingdom has gained a new science minister as part of a broader reshuffle of government posts. Sam Gyimah, who moves from the Ministry of Justice, was appointed minister for universities and science, replacing Jo Johnson. [Nature News] Editorial

Faced with Public Pressure, Research Institutions Step Up Reporting of Clinical Trial Results

The reporting of clinical trial results to a public database — mandated by a ten-year-old federal law — has improved sharply in the last two years, with universities and other nonprofit research centers leading the way. [STAT News] Editorial

NEW The Stem Cell Niche Conference 2018
May 27-31, 2018
Copenhagen, Denmark

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Position – Inflammatory Bowel Disease (KU Leuven)

NEW Postdoctoral Fellowship – Mouse Tumor Biology (Canadian Nuclear Laboratories)

NEW Postdoctoral Research Scientist – Cancer Research (The Cancer Research UK Beatson Institute)

NEW Senior Clinical Researcher – Gastro-Intestinal Pathology (University of Oxford)

Postdoctoral Position – Intestinal Research (University of Pennsylvania)

Research Technician – Translational Cell Assays (Karolinska Institutet)

PhD Studentship – Molecular Probes for Imaging Lysyl Oxidase with Potential Application to the Detection of Metastasis (CRUK Manchester Institute)

Postdoctoral Fellowship – Mouse Tumor Biology (Canadian Nuclear Laboratories)

Postdoctoral Fellow – Immunology (Icahn School of Medicine at Mount Sinai)

PhD Student – Stem Cells of the Intestine and the Stomach (German Cancer Research Center)

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