Intestinal Cell News 5.19 May 31, 2019 | |
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TOP STORYTo better understand the cellular reprogramming which occurs in mutant KRAS cells, investigators undertook a systems-level analysis of four colorectal cancer cell lines which expressed either wild type KRAS or the oncogenic KRASG13D allele (mtKRAS). RNAseq revealed that genes involved in ribosome biogenesis, mRNA translation and metabolism were significantly upregulated in mtKRAS cells. [Br J Cancer] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)Scientists investigated the relationship between fumarate hydratase (FH) and cisplatin (CDDP) sensitivity in gastric cancer cell lines. They found that FH was the most significant gene which induced by CDDP treatment and the suppression of FH could enhance the cytotoxicity of CDDP. [Cell Death Dis] Full Article Using a high-throughput differential scanning fluorimetry assay, researchers identified small-molecule natural products with the capacity to bind the discrete stem-looped structure of pre-miR-21. The most potent compound identified was a prodiginine-type compound, butylcycloheptyl prodiginine, with the ability to inhibit Dicer-mediated processing of pre-miR-21 in vitro. [Cell Chem Biol] Abstract Researchers investigated the applicability of a panel of fluorescent dyes and nanoparticles to label intestinal organoids for visualization using the clinically approved imaging modality, confocal laser endomicroscopy (CLE). Staining homogeneity, durability, cell viability, differentiation capacity, and organoid forming efficiency were evaluated, together with visualization of labeled organoids in vitro and ex vivo using CLE. [Stem Cell Res Ther] Full Article The fraction of adenocarcinomas with submucosa or deeper invasion was higher in AKP270/fl mice than in AKPfl/fl mice, but the incidence of adenocarcinomas per mouse did not differ significantly between AKPfl/fl and AKP270/fl mice. In line with their comparable biological behaviors, mouse primary tumors and tumor-derived organoids with the Trp53R270H or Trp53null alleles had highly similar gene expression profiles. [Lab Invest] Abstract The authors investigated effects of human milk oligosaccharides (hMOs) and non-digestible carbohydrates (NDCs) on human gut epithelial cells by quantifying thickness and area coverage of adsorbed albumin, heparan sulfate and hyaluronic acid in the glycocalyx. Effects of hMOs and NDCs and pectins with degrees of methylation were tested using immunofluorescence staining at one and five days stimulation. [Mol Nutr Food Res] Abstract Investigators showed by global transcriptomic studies that 1,25(OH)2D3 and Wnt3A had profound, additive, partially overlapping effects on the gene expression profile of colorectal (CCD)-18Co human colon myofibroblasts. Moreover, 1,25(OH)2D3 and Wnt3A inhibited CCD-18Co cell proliferation and migration, while 1,25(OH)2D3 reduced, but Wnt3A increased, their capacity to contract collagen gels. [Sci Rep] Full Article Scientists discovered that miR-139-5p targeted the Wnt/β-catenin/TCF7L2 downstream effector E2-2 in colon cancer stem cells (CCSCs). E2-2 is a pivot molecule in the negative feedback loop of miR-139-5p/Wnt/β-catenin/TCF7L2. Its small interfering RNA reversed the stemness maintenance and epithelial-mesenchymal transition of CCSCs. [J Cell Physiol] Abstract The authors investigated the effect of L. sativus-derived diamine oxidase (LSAO) and its mechanism of action on the Caco-2 cell line, considering that LSAO catalizes the oxidative deamination of histamine to the corresponding aldehyde, NH3 and H2O2. Histamine caused a proliferative effect on Caco-2 cells promoting cell migration, invasion and nitric oxide and vascular endothelial growth factor release. [Phytother Res] Abstract Twenty-two ARGs and 18 different plasmid incompatibility types were investigated using polymerase chain reaction and DNA sequencing. In vitro invasion capacity of S. enteritidis isolates possessing different antibiotic resistance patterns were assessed using the Caco2 human intestinal epithelial cell line and biofilm formation was performed in a 96-well polystyrene well format using crystal violet detection. [Microb Drug Resist] Abstract Subscribe to one of our other 19 science newsletters such as Pulmonary Cell News & ESC & iPSC News. | |
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REVIEWSMetabolic Pathways Regulating Colorectal Cancer Initiation and Progression The authors review key findings implicating cancer metabolism as a major contributor of tumor initiation, growth and metastatic dissemination in colorectal cancer. They summarize the metabolic pathways governing stem cell fate in the intestine, the metabolic adaptations of proliferating colon cancer cells and their crosstalk with oncogenic signaling, and how they fulfill the energetic demands imposed by the metastatic cascade. [Semin Cell Dev Biol] Abstract Recent studies have suggested that the c-MET/HGF signaling pathway is involved in the carcinogenesis of colorectal cancer (CRC). Investigators summarize the main findings of recent studies investigating the role of c-MET/HGF signaling pathway in CRC and the potential of the c-MET/HGF signaling pathways in the diagnosis and treatment of CRC. [IUBMB Life] Abstract Visit our reviews page to see a complete list of reviews in the intestinal cell research field. | |
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SCIENCE NEWSResults of a new post-hoc analysis showed that treatment with Orion’s and Bayer’s darolutamide plus androgen deprivation therapy (ADT) delayed worsening of urinary and bowel symptoms maintains quality of life beyond end of study versus placebo plus ADT1. [Press release from Bayer AG (GlobeNewswire, Inc.) discussing research presented at the 2019 American Society of Clinical Oncology (ASCO) Annual Meeting, Chicago] Press Release Treos Bio Limited, an immuno-oncology company developing next-generation, precision cancer vaccines combined with companion diagnostic devices, reported positive interim results from its first-in-man Phase 1/2 study of its precision cancer vaccine with maintenance therapy in patients with metastatic colorectal cancer. [Press release from Treos Bio Limited discussing research presented at the 2019 American Society of Clinical Oncology (ASCO) Annual Meeting, Chicago] Press Release | |
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INDUSTRY NEWSArray BioPharma, Inc. announced positive results from the interim analysis of the Phase III BEACON CRC trial evaluating the combination of BRAFTOVI®, a BRAF inhibitor, MEKTOVI®, a MEK inhibitor, and ERBITUX®, an anti-EGFR antibody, in patients with BRAFV600E-mutant metastatic colorectal cancer, following one or two prior lines of therapy. [Array BioPharma, Inc.] Press Release Progenity, Inc. announced results from three studies that represent potential breakthrough systems for diagnosing, treating, and monitoring digestive diseases through ingestible technologies. Proof-of-concept studies in both human and pre-clinical models showed Progenity’s ingestible diagnostic capsule platform is capable of autonomous location-based sampling, biomolecular analysis, and real-time wireless communications for assessing and monitoring gastrointestinal health. [Progenity, Inc.] Press Release | |
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POLICY NEWSAmbitious Open-Access Plan S Delayed to Let Research Community Adapt A major push by some science agencies to make the research they fund open-access on publication — Plan S — has been delayed by a year. Funders now don’t have to start implementing the initiative until 2021, the agencies announced, to give researchers and publishers more time to adapt to the changes the bold plan requires. [Nature News] Editorial No Paper, No PhD? India Rethinks Graduate Student Policy PhD students in India will no longer be required to publish articles in academic journals before they are awarded their doctorates, if the country’s higher-education regulator adopts recommendations from a committee of researchers. [Nature News] Editorial Bipartisan Bill Would Create Forum for Discussing How to Counter US Academic Espionage Academic leaders say more dialogue is urgently needed on one issue now bedeviling the US research community: how to best protect the country against its economic and military competitors without choking off international scientific collaborations and the free flow of people and ideas. [ScienceInsider] Editorial MD Anderson Clears Researcher Flagged by NIH for Not Disclosing Foreign Ties MD Anderson Cancer Center in Houston, Texas, says it has concluded an investigation of the last of five researchers flagged by the US National Institutes of Health (NIH) for potential violations of agency rules. The investigation “confirmed non-compliance with NIH and MD Anderson rules and policies, but such violations were not in our view serious or indicative of willful malfeasance,” the center said in a statement. [ScienceInsider] Editorial
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EVENTSNEW 19th International Congress of Mucosal Immunology Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Fellow – Immunometabolism of IBD (Luxembourg Institute of Health) Postdoctoral Researcher – Genetics in IBD (Kennedy Institute of Rheumatology) Principle Investigator – Gut Physiology (Nestlé Research Center) Postdoctoral Researcher – Mucosal Immunology and Inflammation (Gilead Sciences, Inc.) Postdoctoral Fellow – Intestinal Regenerative Medicine (Zhou Lab) Postdoctoral Fellow – Intestinal and Pulmonary Stem Cell Biology (Stanford School of Medicine) Postdoctoral Fellow – Functional Genomics of IBS (Wellcome Sanger Institute) Postdoctoral Researcher – Intestinal Immunology (KU Leuven) Postdoctoral Director – mRNA Treatment for Rare Diseases (Moderna) Postdoctoral Researcher – Gastrointestinal Cancer & IBD (Case Western Reserve University) Postdoctoral Position – Oesophago-Gastric Cancer Research (University of Cambridge) Associate Professor – Oncology (University of California, Davis) Postdoctoral Position – Paneth Cells (Erasmus MC Cancer Institute) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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