Intestinal Cell News 6.08 March 6, 2020 | |
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TOP STORYGenome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-β Resistance The authors report improved accuracy and robustness for pooled-library CRISPR screens by capturing sgRNA integrations in single organoids, substantially reducing required cell numbers for genome-scale screening. [Cell Stem Cell] Abstract | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Investigators characterized intestinal stem cell (ISC) and Paneth cell appearance under inflammatory conditions and described the role of mitochondrial function for ISC niche-maintenance. [Gut] Full Article L. reuteri had the ability to maintain the number of Lgr5+ cells and stimulated intestinal epithelial proliferation to repair epithelial damage and reduce proinflammatory cytokine secretion in the intestine and the LPS concentration in serum. [Gut Microbes] Abstract The Proprotein Convertase Furin Is a Pro-Oncogenic Driver in KRAS and BRAF Driven Colorectal Cancer Researchers showed that genetic inactivation of furin suppressed tumorigenic growth, proliferation, and migration in KRAS or BRAF mutant colorectal cancer cell lines but not in wild-type KRAS and BRAF cells. [Oncogene] Abstract Scientists found that HOTAIR expression was markedly upregulated in plasma from colorectal cancer (CRC) patients after radiotherapy and CRC cells after irradiation. HOTAIR knockdown, miR-93 overexpression, or ATG12 silencing weakened cell viability, induced cell apoptosis, inhibited cell autophagy, and enhanced cell radiosensitivity in CRC. [Cell Death Dis] Full Article Mutations in the Coat Complex II Component SEC23B Promote Colorectal Cancer Metastasis The authors showed that deletion of Sec23 homolog B (SEC23B) suppressed the membrane localization of adhesion proteins and augmented cell mobility. SEC23B mutations either caused a premature stop or impaired its protein transport activity. [Cell Death Dis] Full Article In vitro, ChREBP knockdown with siRNA transfection inhibited cell proliferation and induced cell cycle arrest without changes in apoptosis in colon cancer cell lines. [Sci Rep] Full Article Investigators constructed and characterized a 3D in vitro microfluidic model that supported the growth of patient-derived intestinal subepithelial myofibroblasts and endothelial cells into perfused capillary networks. [Sci Rep] Full Article Differentiated Caco-2 cells that contained the native enzyme were used to test maltose hydrolysis. Scientists demonstrated that purified yeast α-glucosidase was non-competitively inhibited by oxyresveratrol and Puag-Haad compared to 153.8 ± 4.3 μg/mL acarbose, an anti-diabetic drug. [Heliyon] Full Article IRBIT Directs Differentiation of Intestinal Stem Cell Progeny to Maintain Tissue Homeostasis Investigators showed that IRBIT expression in intestinal stem cell progeny within the Drosophila midgut epithelium cells regulates their differentiation via suppression of ribonucleotide reductase activity. [iScience] Abstract | Graphical Abstract Subscribe to one of our other 19 science newsletters such as Pulmonary Cell News & ESC & iPSC News. | |
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REVIEWSMetabolic and Immunologic Control of Intestinal Cell Function by mTOR The authors discuss intestinal mammalian/mechanistic target of rapamycin (mTOR) signaling and metabolic regulation in different intestinal cell populations with a special focus on immune cells and their actions on intestinal function. [Int Immunol] Abstract Visit our reviews page to see a complete list of reviews in the intestinal cell research field. | |
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INDUSTRY NEWSInnovation Pharmaceuticals Plans for Phase II Trial of New Treatment for Ulcerative Colitis Innovation Pharmaceuticals, Inc. provided perspectives on its planned Phase II clinical trial of Brilacidin in pill form, its defensin mimetic drug candidate with multi-indication potential, as a novel oral medication for the treatment of ulcerative colitis. [Innovation Pharmaceuticals, Inc.] Press Release NuCana plc announced that the European Medicines Agency’s Committee for Orphan Medicinal Products has issued a positive opinion for orphan drug designation of Acelarin for the treatment of patients with biliary tract cancer. [NuCana plc (GlobeNewswire, Inc.)] Press Release | |
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POLICY NEWSChina Bans Cash Rewards for Publishing Papers Chinese institutions have been told to stop paying researchers bonuses for publishing in journals, as part of a new national policy to cut perverse incentives that encourage scientists to publish lots of papers rather than focus on high-impact work. [Nature News] Editorial UC Santa Cruz Fires Graduate Students Taking Part in Strike The University of California, Santa Cruz, fired 54 graduate student employees from their teaching positions on February 28, in the latest escalation of a months-long dispute over student pay, the Santa Cruz Sentinel reports. [The Scientist] Editorial
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EVENTSNEW Cambridge International Stem Cell Symposium 2020 Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESPhD Student Position – Intestinal Stem Cell Research (Karolinska Institutet) Lab Manager – Intestinal Research (Howard Hughes Medical Institute) Postdoctoral Fellow Position – Gene Editing and Genomic Evolution (Northwestern University) Postdoctoral Position – Inflammatory Bowel Disease (University of Copenhagen) Postdoctoral Fellow – Immunology (Icahn School of Medicine at Mount Sinai) Postdoctoral Position – Intestinal Immunity (The University of Chicago) Postdoctoral Associate – Intestinal Immunology (The Scripps Research Institute) Research Scientist – In Vivo Research on Wnt Signaling in Self-Renewal (Surrozen) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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