Intestinal Cell News Volume 6.42 | Oct 30 2020

May 12, 2022

2020-10-30 | ICN 6.42

Intestinal Cell News by STEMCELL Technologies

Vol. 6.42 – 30 October, 2020

TOP STORY

Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation

Scientists showed that nifedipine, a calcium channel blocker, inhibited calcium influx to impair nuclear factor of activated T cell 2 dephosphorylation, activation, and nuclear translocation, thus preventing transcriptional activation of downstream signaling molecules to suppress colorectal cancer proliferation and metastasis.
[Cell Reports]

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Genome-Wide CRISPR Screen Identifies LGALS2 as an Oxidative Stress-Responsive Gene with an Inhibitory Function on Colon Tumor Growth

Over 600 guide RNAs including the ones targeting LGALS2 were highly enriched in survived cells after sublethal H₂O₂ challenge. DSS-induced colitis model and AOM/dextran sodium sulfate(DSS)-induced colorectal cancer model showed Galectin2(Gal2) loss ameliorated experimental acute colitis but promoted tumor growth. Mechanically, Gal2 deficiency activated STAT3, promoting colorectal tumor cell growth. Overall, the researchers supported a suppressive role of Gal2 in the colorectal tumor development.
[Oncogene]

Full Article

NF-κB Maintains the Stemness of Colon Cancer Cells by Downregulating miR-195-5p/497–5p and Upregulating MCM2

Cancer stem cells were isolated from human colon cancer cell lines, where the stemness of the cells was evaluated by cell viability, colony-forming, spheroid-forming, invasion, migration, and apoptosis assays.
[Journal of Experimental & Clinical Cancer Research]

Full Article

NDRG2 Regulates Adherens Junction Integrity to Restrict Colitis and Tumorigenesis

Intestine-specific N-myc downstream-regulated gene 2 (Ndrg2) deficiency mice were subjected to DSS- or TNBS-induced colitis, and AOM-DSS-induced colitis-associated tumor. HT29 cells, Caco2 cells, primary intestinal epithelial cells from Ndrg2ΔIEC mice, mouse embryo fibroblasts from systemic Ndrg2 knockout mice, HEK293 cells and human UC and DC specimens were used to investigate NDRG2 function in colitis and colitis-associated tumor.
[EBioMedicine]

Full Article

The Long Noncoding RNA LUCAT1 Promotes Colorectal Cancer Cell Proliferation by Antagonizing Nucleolin to Regulate MYC Expression

Investigators showed that the interaction between LUCAT1 and Nucleolin (NCL) interferes NCL-mediated inhibition of MYC and promote the expression of MYC. Cells lacking LUCAT1 show a decreased MYC expression, and NCL knockdown rescue LUCAT1 depletion-induced inhibition of colorectal cancer cell proliferation and MYC expression.
[Cell Death & Disease]

Full Article

Targeting Tumor Cell-Derived CCL2 as a Strategy to Overcome Bevacizumab Resistance in ETV5⁺ Colorectal Cancer

Gene set enrichment analysis and additional assays confirmed that ETV5 could promote angiogenesis by inducing the secretion of another tumor angiogenesis factor (CCL2) in CRC cells to facilitate Bevacizumab resistance.
[Cell Death & Disease]

Full Article

Leptin Downregulates Angulin-1 in Active Crohn’s Disease via STAT3

Using human biopsies, scientists found angulin-1 was downregulated in active Crohn’s disease compared with both controls and Crohn’s disease in remission. In T84 and Caco-2 monolayers, leptin, a cytokine secreted by fat tissue and affected in Crohn’s disease, decreased angulin-1 expression. This effect was completely blocked by STAT3 inhibitors, Stattic and WP1066, but only partially by JAK2 inhibitor AG490.
[International Journal of Molecular Sciences]

Full Article

Significance of Aldo-Keto Reductase 1C3 and ATP-Binding Cassette Transporter B1 in Gain of Irinotecan Resistance in Colon Cancer Cells

Researchers established CPT11-resistant variants of three human colon cancer cell lines, and found that gain of the resistance elicited an up-regulation of aldo-keto reductase 1C3 in the cells.
[Chemico-Biological Interactions]

Abstract Graphical Abstract

Exogenous L-Arginine Increases Intestinal Stem Cell Function through CD90⁺ Stromal Cells Producing mTORC1-Induced Wnt2b

Scientists utilized mice and small intestinal organoid models to clarify the role of L-arginine on epithelial differentiation of intestinal stem cells (ISCs). They showed that L-arginine increased expansion of ISCs in mice.
[Communications Biology]

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REVIEWS

Intestinal Organoid/Enteroid-Based Models for Cryptosporidium

The authors discuss recent breakthroughs in the field and highlight different models for functional ex vivo organoid or enteroid derived culture systems.
[Current Opinion in Microbiology]

Full Article

INDUSTRY AND POLICY NEWS

ENHERTU^® Granted Priority Review in the US for Treatment of HER2 Positive Metastatic Gastric Cancer

Daiichi Sankyo Company, Limited and AstraZeneca’s ENHERTU has received acceptance for its supplemental Biologics License Application and has also been granted Priority Review in the US for the treatment of patients with HER2 positive metastatic gastric or gastroesophageal junction adenocarcinoma.
[Daiichi Sankyo Company, Ltd]

Press Release

FEATURED EVENT

Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation

Genome-Wide CRISPR Screen Identifies LGALS2 as an Oxidative Stress-Responsive Gene with an Inhibitory Function on Colon Tumor Growth

NF-κB Maintains the Stemness of Colon Cancer Cells by Downregulating miR-195-5p/497–5p and Upregulating MCM2

NDRG2 Regulates Adherens Junction Integrity to Restrict Colitis and Tumorigenesis

The Long Noncoding RNA LUCAT1 Promotes Colorectal Cancer Cell Proliferation by Antagonizing Nucleolin to Regulate MYC Expression

Targeting Tumor Cell-Derived CCL2 as a Strategy to Overcome Bevacizumab Resistance in ETV5⁺ Colorectal Cancer

Leptin Downregulates Angulin-1 in Active Crohn’s Disease via STAT3

Significance of Aldo-Keto Reductase 1C3 and ATP-Binding Cassette Transporter B1 in Gain of Irinotecan Resistance in Colon Cancer Cells

Exogenous L-Arginine Increases Intestinal Stem Cell Function through CD90⁺ Stromal Cells Producing mTORC1-Induced Wnt2b

Intestinal Organoid/Enteroid-Based Models for Cryptosporidium

ENHERTU^® Granted Priority Review in the US for Treatment of HER2 Positive Metastatic Gastric Cancer

Keystone Symposia: Tumor Metabolism and the Microenvironment

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Postdoctoral Fellow – Breast and Colorectal Cancer

Postdoctoral Research Associate – Colorectal Cancer

Postdoctoral Associate – Microbiome Analytics

Senior Research Technologist – Inflammation and Immunity

Research Associate – Ex Vivo and In Vivo Models of Colorectal Cancer

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