Intestinal Cell News Volume 7.00 | Jan 8 2021

May 12, 2022

2020-08-01 | ICN 7.00

Intestinal Cell News by STEMCELL Technologies

Vol. 7.00 – 8 January, 2021

TOP STORY

Spatiotemporal
Analysis of Human Intestinal Development at Single-Cell Resolution

Scientists linked single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. They identified 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones.
[Cell]

Full Article Graphical Abstract

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PUBLICATIONSRanked by the impact factor of the journal

Colorectal
Cancer Cells Enter a Diapause-Like DTP State to Survive Chemotherapy

Investigators combined cellular barcoding and mathematical modeling in patient-derived colorectal cancer models to identify and characterize drug-tolerant persisters (DTPs) in response to chemotherapy.
[Cell]

Abstract Graphical Abstract

The
Amino Acid Transporter SLC7A5 Is Required for Efficient Growth of KRAS-Mutant Colorectal Cancer

The authors showed that concomitant mutation of Apc and Kras in the mouse intestinal epithelium profoundly rewired metabolism, increasing glutamine consumption.
[Nature Genetics]

Abstract

Establishment
of Intestinal Organoid Cultures Modeling Injury-Associated Epithelial Regeneration

Researchers established a novel intestinal organoid culture system composed of eight components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury.
[Cell Research]

Full Article

Non-Canonical
Wnt/PCP Signaling Regulates Intestinal Stem Cell Lineage Priming towards Enteroendocrine and Paneth Cell Fates

Scientists discovered non-canonical Wnt/planar cell polarity-activated intestinal stem cells that were primed towards the enteroendocrine or Paneth cell lineage.
[Nature Cell Biology]

Abstract

A RAC-GEF Network Critical for Early Intestinal Tumorigenesis

Transcriptional profiling of Apc deficient intestinal tissue identified Vav3 and Tiam1 as key targets.
[Nature Communications]

Full Article

Chromatin
Regulatory Dynamics of Early Human Small Intestinal Development Using a Directed Differentiation Model

Researchers leveraged chromatin run-on sequencing to define the landscape of active promoters, enhancers and gene bodies across distinct stages of directed differentiation of human pluripotent stem cells into small intestinal spheroids with regional specification.
[Nucleic Acids Research]

Full Article

Homeostatic
Regulation of ROS-Triggered Hippo-Yki Pathway via Autophagic Clearance of Ref(2)P/p62 in the Drosophila Intestine

Scientists showed that in Drosophila intestine autophagy lowered the sensitivity of differentiated enterocytes to reactive oxygen species that were produced in response to commensal bacteria.
[Developmental Cell]

Full Article Graphical Abstract

E7386,
a Selective Inhibitor of the Interaction between β-Catenin and CBP, Exerts Antitumor Activity in Tumor Models with Activated Canonical Wnt Signaling

E7386 demonstrated clear antitumor activity via modulation of the Wnt/β-catenin signal pathway and alteration of the tumor and immune microenvironments, and its antitumor activity could be enhanced in combination with anti-PD-1 antibody.
[Cancer Research]

Abstract

Bach2
Deficiency Promotes Intestinal Epithelial Regeneration by Accelerating DNA Repair in Intestinal Stem Cells

Researchers found that Bach2 deficiency promoted intestinal epithelial cell proliferation during homeostasis. Moreover, genetic inactivation of Bach2 in mouse intestinal epithelium facilitated crypt regeneration after irradiation, resulting in a reduction in mortality.
[Stem Cell Reports]

Full Article Graphical Abstract

Evaluation
of Tissue Stem Cell Derived Human Intestinal Organoids, a Physiologically Relevant Model to Evaluate Cytochrome P450 Induction in Gut

Matched human enteroid and colonoid lines, generated from ileal and colon biopsies from two donors, were cultured in extracellular matrix for three days, followed by a single 48 hour treatment with rifampin, omeprazole, CITCO and phenytoin at concentrations that induced target genes in hepatocytes.
[Drug Metabolism and Disposition]

Abstract

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REVIEWS

Organoid-Based
Models to Study the Role of Host-Microbiota Interactions in IBD

Scientists review different organoid-based ex vivo models that are currently available, and benchmark their suitability and limitations for specific research questions.
[Journal of Crohns & Colitis]

Abstract

INDUSTRY AND POLICY NEWS

European Medicines Agency Validates Bristol Myers Squibb’s Application for Opdivo (Nivolumab) Combined with Chemotherapy as First-Line Treatment in Metastatic Gastric Cancer, Gastroesophageal Junction Cancer and Esophageal Adenocarcinoma

Bristol Myers Squibb announced that the European Medicines Agency validated its Type II Variation Marketing Authorization Application for Opdivo in combination with fluoropyrimidine- and platinum-based combination chemotherapy for the first-line treatment of adult patients with advanced or metastatic gastric cancer, gastroesophageal junction cancer or esophageal adenocarcinoma.
[Bristol Myers Squibb]

Press Release

FEATURED EVENT

Spatiotemporal
Analysis of Human Intestinal Development at Single-Cell Resolution

Colorectal
Cancer Cells Enter a Diapause-Like DTP State to Survive Chemotherapy

The
Amino Acid Transporter SLC7A5 Is Required for Efficient Growth of KRAS-Mutant Colorectal Cancer

Establishment
of Intestinal Organoid Cultures Modeling Injury-Associated Epithelial Regeneration

Non-Canonical
Wnt/PCP Signaling Regulates Intestinal Stem Cell Lineage Priming towards Enteroendocrine and Paneth Cell Fates

A RAC-GEF Network Critical for Early Intestinal Tumorigenesis

Chromatin
Regulatory Dynamics of Early Human Small Intestinal Development Using a Directed Differentiation Model

Homeostatic
Regulation of ROS-Triggered Hippo-Yki Pathway via Autophagic Clearance of Ref(2)P/p62 in the Drosophila Intestine

E7386,
a Selective Inhibitor of the Interaction between β-Catenin and CBP, Exerts Antitumor Activity in Tumor Models with Activated Canonical Wnt Signaling

Bach2
Deficiency Promotes Intestinal Epithelial Regeneration by Accelerating DNA Repair in Intestinal Stem Cells

Evaluation
of Tissue Stem Cell Derived Human Intestinal Organoids, a Physiologically Relevant Model to Evaluate Cytochrome P450 Induction in Gut

Organoid-Based
Models to Study the Role of Host-Microbiota Interactions in IBD

European Medicines Agency Validates Bristol Myers Squibb’s Application for Opdivo (Nivolumab) Combined with Chemotherapy as First-Line Treatment in Metastatic Gastric Cancer, Gastroesophageal Junction Cancer and Esophageal Adenocarcinoma

ISSCR: Stem Cells and Global Sustainability

> See All Events

Scientific
Communities & Communications Specialist

Postdoctoral
Researcher – Somatic Origin of Cancer

Postdoctoral Researcher – Single-Cell Immunology

Postdoctoral Fellow – Blood-Based Biomarkers

Postdoctoral
Research Fellow – Kinase Function in Inflammatory Bowel Disease

Director,
GMA Oncology – Multi-Tumor Strategy

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