Intestinal Cell News Volume 8.07 | Feb 25 2022

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    2022-02-25 | ICN 8.07


    Intestinal Cell News by STEMCELL Technologies
    Vol. 8.07 – 25 February, 2022
    TOP STORY

    Generation of CRISPR–Cas9-Mediated Genetic Knockout Human Intestinal Tissue–Derived Enteroid Lines by Lentivirus Transduction and Single-Cell Cloning

    Researchers provided a detailed technical pipeline and protocol for using the CRISPR–Cas9 genome editing system to knock out a gene of interest specifically in human intestinal tissue-derived enteroids by lentiviral transduction and single-cell cloning.
    [Nature Protocols]

    Abstract

    Watch this innovation showcase from ISSCR 2021 on intestinal organoids as models for drug development.
    PUBLICATIONSRanked by the impact factor of the journal

    Glycemia and Body Weight Are Regulated by Sodium-Glucose Cotransporter 1 (SGLT1) Expression via O-GlcNAcylation in the Intestine

    Scientists revealed the roles of O-GlcNAcylation in glucose absorption by the small intestine and elucidated the mechanism by which O-GlcNAcylation regulated sodium-glucose cotransporter 1 (SGLT1) expression.
    [Molecular Metabolism]

    AbstractFull ArticleGraphical Abstract

    CCDC12 Promotes Tumor Development and Invasion through the Snail Pathway in Colon Adenocarcinoma

    Knockdown of CCDC12 could significantly reduce proliferation, migration, invasion, and tumorigenicity of colon cancer cells, while exogenous overexpression of CCDC12 had the opposite effect.
    [Cell Death & Disease]

    Full Article

    Curcumin Mitigates TNFα-Induced Caco-2 Cell Monolayer Permeabilization through Modulation of NF-κB, ERK1/2 and JNK Pathways

    Caco-2 cell monolayers were incubated with TNFα, in the absence or presence of curcumin. TNFα caused an increase in interleukin (IL)-6 and IL-8 release which was inhibited by curcumin in a dose-dependent manner.
    [Molecular Nutrition & Food Research]

    Abstract

    Lecanoric Acid Mediates Anti-Proliferative Effects by an M Phase Arrest in Colon Cancer Cells

    Scientists performed cytotoxicity, cell colony, cell cycle and apoptosis assays in various cell lines or primary immune cells.
    [Biomedicine & Pharmacotherapy]

    Full ArticleGraphical Abstract

    Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway

    Cucurbitacin E (CE) belongs to the triterpenoids and has received substantial attention for its antineoplastic property. The authors revealed that CE significantly induced cellular senescence in colorectal cancer cells.
    [Journal of Agricultural and Food Chemistry]

    AbstractGraphical Abstract

    The E3 Ubiquitin Ligase, FBXW5, Promotes the Migration and Invasion of Gastric Cancer through the Dysregulation of the Hippo Pathway

    Blocking large tumor suppressor kinases 1/2-YAP1 led to the loss of F-box and WD repeat domain-containing 5 (FBXW5)-mediated regulation of the Hippo pathway and partial functions.
    [Cell Death Discovery]

    Full Article

    ATR Inhibitor AZD6738 Increases the Sensitivity of Colorectal Cancer Cells to 5-Fluorouracil by Inhibiting Repair of DNA Damage

    In vitro, the AZD6738/5‑fluorouracil combination increased the number of mitotic cells according to flow cytometry, decreased the checkpoint kinase 1 phosphorylation levels and increased cleaved caspase‑3 and phosphorylated form of H2A.X variant histone levels according to western blotting, and decreased the proliferation rate of four colon cancer cell lines according to cell viability experiments.
    [Oncology Reports]

    Abstract

    Differential Expression of HNF1A and HNF1A-AS1 in Colon Cancer Cells

    Scientists determined the epigenetic features of the HNF1A gene loci, and expression and cellular localization of HNF1A-AS1 RNA, HNF1A RNA, and HNF1A protein in colorectal cancer and normal colon epithelial cells.
    [IUBMB Life]

    Abstract

    lncRNA TUG1 Protects Intestinal Epithelial Cells from Damage Induced by High Glucose and High Fat via AMPK/SIRT1

    Cell Counting Kit‑8 assays were performed to analyze cell viability, and flow cytometry and a TUNEL assay was performed to evaluate cell apoptosis.
    [Molecular Medicine Reports]

    Abstract

    Request your free copy of the 'Directed Differentiation of Pluripotent Stem Cells' Wallchart
    REVIEWS

    A Review of the Therapeutic Management of Crohn’s Disease

    The authors discuss the key milestones in the medical management of Crohn’s disease.
    [Therapeutic Advances in Gastroenterology]

    Full Article

    INDUSTRY AND POLICY NEWS

    NovoBiome and Inserm Form New Collaboration to Simulate Human Intestinal Physiology

    NovoBiome announced that it has signed a new collaboration with the French National Institute of Health and Medical Research (Inserm), a scientific and technological institute which operates under the joint authority of the French Ministries of Health and Research.
    [NovoBiome, Inc. (GlobeNewswire, Inc.)]

    Press Release

    FEATURED EVENT

    Antibody Biology and Engineering

    March 13 – 18, 2022
    Ventura, California, United States

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    JOB OPPORTUNITIES

    Senior Research Scientist II – Therapeutics

    Gilead Sciences, Inc. – Foster City, California, United States

    Postdoctoral Researcher – Intestinal Organoid

    Baylor College of Medicine – Houston, Texas, United States

    Postdoctoral Positions – Molecular Cancer Research

    University of Copenhagen – Copenhagen, Denmark

    Research Associate – Gastroenterology

    The University of British Columbia – Vancouver, British Columbia, Canada

    Postdoctoral Fellow – Intestinal Inflammation

    University of North Dakota – Grand Forks, North Dakota, United States

    > See All Jobs

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