Mesenchymal Cell News 10.36 September 11, 2018 | |
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TOP STORYIn Vivo Reprogramming of Wound-Resident Cells Generates Skin Epithelial Tissue Investigators generated expandable epithelial tissues using in vivo reprogramming of wound-resident mesenchymal cells. Transduction of four transcription factors that specified the skin-cell lineage enabled efficient and rapid de novo epithelialization from the surface of cutaneous ulcers in mice. [Nature] Abstract | Press Release | |
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PUBLICATIONS(Ranked by impact factor of the journal)IN VITRORhoA/ROCK Signaling Modulates Lineage Commitment of Mesenchymal Stem Cells in Asthma through Lef1 Expression of constitutively active RhoA (RhoA-L63) facilitated the differentiation of MSCs to fibroblasts/myofibroblasts, whereas expression of dominant negative RhoA switched the differentiation toward epithelial cells. Gene array identified the Wnt signaling effector Lef1 as the most up-regulated gene in RhoA-L63-transfected MSCs. Knockdown of Lef1 induced MSC differentiation away from fibroblasts/myofibroblasts but towards epithelial cells. [J Allergy Clin Immunol] Abstract Researchers found that the hydrogel from acellular porcine adipose tissue (HAPA) in combination with adipose-derived stem cells (ADSCs) could induce in situ adipogenesis in the wound microenvironment. The newly regenerated adipocytes enhanced fibroblast migration, accelerated wound closing, and enhanced wound epithelialization. Newly formed, intact skin structure was observed after treating the wound with ADSCs-loaded HAPA. [J Invest Dermatol] Abstract MSC-Seeded Biomimetic Scaffolds as a Factory of Soluble RANKL in Rankl-Deficient Osteopetrosis Magnesium-doped hydroxyapatite/collagen I-seeded murine MSCs showed improved properties, as compared to two-dimensional culture, in terms of proliferation and human soluble RANKL (hsRL) production, with respect to lentiviral hsRL‐transduced cells. When implanted subcutaneously in Rankl−/− mice, these cell constructs were well tolerated, colonized by host cells, and intensely vascularized. [Stem Cells Transl Med] Full Article | Press Release Spatially defined SOX9 overexpression enhanced proliferative, metabolic, and chondrogenic activities compared with control treatment. Administration of a SOX9 vector was also capable of delaying premature hypertrophic and osteogenic differentiation in constructs. [Int J Mol Sci] Full Article Exosomal miR-134 significantly inhibited apoptosis by decreasing caspase-8 expression and activity in an oxygen-glucose deprivation-treated group cultured with bone marrow-derived MSC-exosomes and oligodendrocytes. [J Cell Biochem] Abstract IN VIVOTransmissible ER Stress Reconfigures the AML Bone Marrow Compartment Investigators observed that extracellular vesicles transmitted endoplasmic reticulum (ER) stress in vivo from acute myeloid leukemia (AML) xenografts to bone marrow stroma, whereby the upregulation of core unfolded protein response components drove subsequent osteolineage differentiation of MSCs. [Leukemia] Abstract Scientists determined that ablation of the alkaline phosphatase gene (Alpl) induced prototypical premature bone aging characteristics, including bone mass loss and marrow fat gain coupled with elevated expression of p16INK4A and p53 due to senescence and impaired differentiation in MSCs. [Bone Res] Full Article Transient apoptosis was found to be due to the expression of a miniature swine maxillofacial-specific miRNA, ssc-mir-133b. Upregulation of ssc-mir-133b resulted in robust apoptosis in primary dental MSCs in maxillofacial region. An animal study demonstrated that ectopic expression of ssc-mir-133b resulted in failed tooth formation after twelve weeks of subcutaneous transplantation in nude mice. [Bone Res] Full Article Quercetin Inlaid Silk Fibroin/Hydroxyapatite Scaffold Promoting Enhanced Osteogenesis Quercetin (low-content)/silk fibroin/hydroxyapatite (Qtn/SF/HAp) scaffold was found to be an efficient cell carrier facilitating cellular growth, proliferation and osteogenic differentiation as compared to SF/HAp and Qtn (high-content)/SF/HAp scaffolds. However Qtn (high-content)/SF/HAp was observed to inhibit cell proliferation without any effects on cell viability. [ACS Appl Mater Interfaces] Abstract Placenta-derived MSCs (P-MSCs) had greater migratory activity towards glioblastoma stem-like cells and across the blood-brain barrier compared with bone marrow-derived MSCs, and this activity was enhanced by hypoxia precondition. Polyethylene glycol-coated superparamagnetic iron oxide was synthesized for cellular labeling and imaging P-MSCs, displaying high cellular uptake and no cytotoxic effect on P-MSCs cell proliferation or stemness property. [Artif Cells Nanomed Biotechnol] Abstract Subscribe to one of our other 19 science newsletters such as ESC & iPSC News & Cell Therapy News. | |
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REVIEWSThe authors introduce the latest advances, methods, and applications of MSCs in gene therapy of various malignant organ disorders. Additionally, they cover the problems and challenges which researchers have faced with when trying to translate their basic experimental findings in MSCs research to clinically applicable therapeutics. [J Cell Physiol] Abstract Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field. | |
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INDUSTRY NEWSCCRM and JSRM sign MOU to accelerate Canadian and Japanese advances in RM CCRM and the Japanese Society for Regenerative Medicine (JSRM) have agreed to collaborate to advance the field of regenerative medicine (RM) and cell and gene therapies in Canada and Japan, signing a Memorandum of Understanding (MOU). [CCRM] Press Release Histogenics Corporation announced that its Phase III clinical trial of NeoCart did not meet the primary endpoint of a statistically significant improvement in pain and function in a dual threshold responder analysis one year after treatment as compared to microfracture. [Histogenics] Press Release | |
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POLICY NEWSAdvocate for Women in Science Wins Prestigious Lasker Award Pioneers in anaesthesiology, DNA structure and RNA biology are this year’s Lasker Awards recipients. The annual prize rewards achievements in biomedical research and is often considered a precursor to the Nobel. [Nature News] Editorial Top Cancer Researcher Called Out for Not Disclosing Conflicts in Medical Journals Medical journals and professional societies require study authors and presenters to disclose conflicts of interest, but this didn’t stop José Baselga, M.D., who heads up research at Memorial Sloan Kettering Cancer Center, from fudging his disclosures. [Fierce Biotech] Editorial Peer Reviewers in Developing Nations Are Underrepresented, Report Suggests Combined, scientists spend nearly 70 million hours peer reviewing manuscripts for scholarly journals every year, a new report says. But too much of the onus rests on researchers in wealthy countries, according to the report by the company Publons—based in London and Wellington—which enables researchers to track and claim credit for the peer reviews they perform. [ScienceInsider] Editorial
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EVENTSNEW Gordon Research Seminar: Unifying Cartilage Biology Research: Bone Development, Cartilage Physiology and Pathology Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Assistant Professor – Stem Cell and Regenerative Biology (Columbia University) Post-Doctoral Researcher – Mesenchymal Stem Cell Therapy in Severe Asthma (Maynooth University) Postdoctoral Researcher – Interdisciplinary (Oregon Health & Science University) Faculty Positions – Biomedical Research (Memorial Sloan Kettering Cancer Center) Postdoctoral Research Fellow – Spinal Fusion (Hopkins Spinal Fusion Laboratory) Senior Lecturer – Stromal Immunology (University of Aberdeen) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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