Mesenchymal Cell News Volume 11.15 | Apr 23 2019

TOP STORY

Welding with Stem Cells for Next-Generation Surgical Glues

Researchers modified the membrane of human MSCs with an enzyme, known as thrombin, which is involved in the wound healing process. When the modified cells were placed in a solution containing the blood protein fibrinogen, they automatically welded together through the growth of a natural hydrogel from the surface of the cells. [Press release from the University of Bristol discussing online prepublication in Nature Communications] Press Release | Full Article

(Ranked by impact factor of the journal)

IN VITRO

A Microparticle Approach for Non-Viral Gene Delivery within 3D Human Mesenchymal Stromal Cell Aggregates

Scientists developed a highly efficient microparticle-based gene delivery approach to uniformly transfect human MSCs (hMSCs) within multicellular aggregates and cell sheets. They hypothesized that release of plasmid DNA (pDNA) lipoplexes from mineral-coated microparticles within 3D hMSC constructs would improve transfection in comparison to standard free pDNA lipoplex delivery in the media. [Acta Biomater] Abstract

Inhibition of Cyclin-Dependent Kinase 4 as a Potential Therapeutic Strategy for Treatment of Synovial Sarcoma

The authors reported that CDK4 was highly expressed in human synovial sarcoma, and high CDK4 expressions were associated with poor prognosis in sarcoma patients and the clinical stage and the TNM grade in synovial sarcoma patients. Knockdown of CDK4 with specific small interference RNAs inhibited cell proliferation and enhances apoptotic effects in synovial sarcoma cells. [Cell Death Dis] Full Article

Nicotinamide Mononucleotide Promotes Osteogenesis and Reduces Adipogenesis by Regulating Mesenchymal Stromal Cells via the SIRT1 Pathway in Aged Bone Marrow

Scientists identified that nicotinamide mononucleotide (NMN), a key natural NAD⁺ intermediate, effectively encouraged MSC expansion in vitro and in vivo. The in vitro expanded MSCs had heightened osteogenesis, but reduced adipogenesis. Furthermore, NMN supplementation stimulated osteogenesis of endogenous MSCs, and protected bone from aging and irradiation induced damage in mice. [Cell Death Dis] Full Article

Metabolites Can Regulate Stem Cell Behavior through the STAT3/AKT Pathway in a Similar Trend to That under Hypoxic Conditions

Researchers selected several metabolites to examine the relation between metabolites and stem cell functions. In addition, they investigated the ability of selected metabolites to induce rapid expansion of this cell population. [Sci Rep] Full Article

Mesenchymal Stem Cells Increase Alveolar Differentiation in Lung Progenitor Organoid Cultures

To understand the interactions between MSCa and distal lung epithelial cells, investigators added MSCs to lung progenitor 3D cultures. MSCs stimulated Epcam⁺ Sca-1⁺ derived organoid formation, increased alveolar differentiation and decreased self-renewal. [Sci Rep] Full Article

Cytokines Secreted from Bone Marrow Derived Mesenchymal Stem Cells Promote Apoptosis and Change Cell Cycle Distribution of K562 Cell Line as Clinical Agent in Cell Transplantation

Scientists evaluated the influence of BMSCs on the K562 cell line as chronic myeloid leukemia cells, with the use of a cytokine antibody array recognizing 34 cytokines. For this purpose, BMSCs were isolated and co-cultured with K562 cells; thereafter, cultured K562 alone and co-cultured K562 with BMSCs were collected at day seven and subjected to cell cycle distribution assay as well as annexin/PI analysis and Ki/caspase-3 assay for apoptosis assessment. [PLoS One] Full Article

Green Propolis Extract Promotes In Vitro Proliferation, Differentiation, and Migration of Bone Marrow Stromal Cells

The cytocompatibility of propolis extract and the proliferation of bone marrow mesenchymal stromal cells (BMMSCs) in the presence of propolis was evaluated by live/dead cell staining and MTS viability assay over a period of three days. Also, researchers evaluated the effect of propolis extract on trilineage differentiation and migration capacity of undifferentiated and differentiated BMMSCs. [Biomed Pharmacother] Full Article

IN VIVO

Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content

Scientists compared various preconditioning regimens for both the quantitative and qualitative production of MSC-derived extracellular vesicles (EVs), and their therapeutic efficacy for proangiogenic activity in vitro and cutaneous wound healing in vivo. After preconditioning with thrombin, H₂O₂, lipopolysaccharide, or hypoxia, EV secretion was assessed quantitatively by measuring production per cell and protein quantification, and qualitatively by measuring a proteome profiler and an enzyme-linked immunosorbent assay contained within EVs. [J Clin Med] Full Article

Proteomic Analysis of Human Mesenchymal Stromal Cell Secretomes: A Systematic Comparison of the Angiogenic Potential

Investigators compared angiogenesis of human MSC (hMSC) secretomes from adipose tissue, bone marrow, and umbilical cord Wharton’s jelly. hMSC secretomes were obtained under xenofree conditions and analyzed by liquid chromatography tandem mass spectrometry. Biological processes related to angiogenesis were found to be enriched in the proteomic profile of hMSC secretomes. [npj Regen Med] Full Article

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Mesenchymal Stromal Cells for Ocular Surface Repair

The authors explore the pathogenesis of corneal disorders in response to injury and disease, and the potential for MSCs to modulate this process as a treatment. Through the review of over twenty-five animal studies, they investigate the common mechanisms of action by which MSCs have their effect and discuss their potential for treating and/or preventing corneal deterioration. [Expert Opin Biol Ther] Abstract

Empowering Mesenchymal Stem Cells for Ocular Degenerative Disorders

Investigators discuss the current and feasible strategy options to enhance treatment outcomes with MSC therapy. They review the application of various types of biomaterials and advances in nanotechnology, which have been employed on MSCs to augment cellular function and differentiation for improving treatment of visual functions. [Int J Mol Sci] Full Article

Mesenchymal Stem Cells and Their Mitochondrial Transfer: A Double-Edged Sword

Scientists summarize the research on MSCs and their mitochondrial transfer. They provide an overview of the biology, function, niches and signaling that play a role in tissue repair. They also highlight the pathologies of cancer growth and metastasis linked to mitochondrial exchange between cancer cells and surrounding stromal cells. [Biosci Rep] Abstract | Full Article

Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field.

Ipsen Completes Acquisition of Clementia Pharmaceuticals

Ipsen and Clementia Pharmaceuticals announced the closing of Ipsen’s acquisition of Clementia following approval of the arrangement by Clementia shareholders and the Quebec Superior Court. [Ipsen Pharma] Press Release

AANS 2019: SB623 Demonstrated Statistically Significant Improvement in Motor Function Among Patients with Chronic Motor Deficit from Traumatic Brain Injury (TBI)

The SanBio Group announced that SB623 met its primary endpoint, with patients treated with SB623 achieving an average 8.7 point improvement from baseline in the FMMS, versus 2.4 in the control group, at 24 weeks, in a Phase II trial of patients with chronic motor deficit from TBI. [SanBio Co, Ltd.] Press Release

Three Researchers Ousted from MD Anderson

MD Anderson Cancer Center has ousted three researchers for violating granting agency policies, reports The Houston Chronicle. Last year, the National Institutes of Health notified the center of five grantees there who were under investigation, reports the Chronicle. The senior researchers violated confidentiality of peer review and disclosure of connections to foreign countries, specifically China, according to Science. [The Scientist] Editorial

Indian Scientists Launch Preprint Repository to Boost Research Quality

Researchers in India will soon have their own preprint repository where they can post manuscripts from any discipline. The founders of IndiaRxiv hope it will improve the quality of science in the country. [Nature News] Editorial

NEW ISSCR 2019 Annual Meeting
June 26-29, 2019
Los Angeles, California, United States

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Fellow – Sarcoma Image Processing (Institute of Cancer Research)

NEW Lecturer – Stem Cell Biology & Regenerative Medicine (University of Manchester)

Postdoctoral Fellow – Sarcoma Genomics Analysis (The Francis Crick Institute)

Postdoctoral – Stem Cell & Biochemical Pharmacology (Genetech)

Senior Research Fellow – Stem Cell & Cancer Research (Gillies McIndoe Research Institute)

Postdoctoral Fellowship – Molecular Oncology (Max Delbrück Center for Molecular Medicine)

Junior Professorship – Translational Pediatric Sarcoma Research (German Research Center)

Cell Biologist – MSCs & Liver Disease (Hepatx Corporation)

Postdoctoral Fellow & Research Associate – Morphogenetic Signaling Pathways (University of Rochester Medical Center)

Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California at Davis)

Assistant Professor – Department of Biological Sciences (Rutgers University Newark)

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