Mesenchymal Cell News Volume 3.10 | Mar 15 2011

The Impact of Inflammatory Licensing on Heme Oxygenase-1–Mediated Induction of Regulatory T Cells by Human Mesenchymal Stem Cells
As heme oxygenase-1 (HO-1) has been implicated in the induction of regulatory T-cells (Tregs), researchers sought to examine its impact on mesenchymal stem cell-driven promotion of Tregs. [Blood]

The Effect of Matrix Stiffness on the Differentiation of Mesenchymal Stem Cells in Response to TGF-Beta
Transforming growth factor beta (TGF-beta) can promote mesenchymal stem cell differentiation into either smooth muscle cells or chondrogenic cells. Here researchers showed that the stiffness of cell adhesion substrates modulated these differential effects. [Biomaterials]

TGF Beta Transduced Mesenchymal Stem Cells Ameliorate Autoimmune Arthritis through Reciprocal Regulation of Treg-Th17 Cells and Osteoclastogenesis
Researchers examined the therapeutic potential of TGF beta-transduced bone marrow-derived mesenchymal stem cells in experimental autoimmune arthritis, using an accepted animal model of collagen-induced arthritis. [Arthritis Rheum]

Human Primary Bone Sarcomas Contain CD133+ Cancer Stem Cells Displaying High Tumorigenicity In Vivo
Researchers selected a CD133+ subpopulation from stabilized cell lines that displayed the capacity to grow as sarcospheres able to initiate and sustain tumor growth in nonobese diabetic/severe combined (NOD/SCID) mice, to express stemness genes, including OCT3/4, Nanog, Sox2, and Nestin, and to differentiate into mesenchymal lineages, such as osteoblasts and adipocytes. [FASEB J]

hMSC-Mediated Concurrent Delivery of Endostatin and Carboxylesterase to Mouse Xenografts Suppresses Glioma Initiation and Recurrence
Researchers established three genetically engineered mesenchymal stem cell (MSC) lines (GE-AF-MSCs) by stably transducing the gene encoding endostatin (an antiangiogenesis factor), the gene encoding secretable form of carboxylesterase 2 (sCE2, a prodrug-activating enzyme), or a mixture of both genes. [Mol Ther]

A Multicellular Approach Forms a Significant Amount of Tissue-Engineered Small Intestine in the Mouse
Researchers demonstrate the preservation of Lgr5 and DcamKl1 positive cells, two putative intestinal stem cell populations, in close proximity to their niche mesenchymal cells, the intestinal subepithelial myofibroblasts at the time of implantation. [Tissue Eng Part A]

Analysis of Post Transcriptional Regulation of SOX9 mRNA During In Vitro Chondrogenesis
Researchers dedifferentiated human articular chondrocytes in monolayer culture before placing them in chondrogenic 3-dimensional pellet cultures. At the same time, researchers induced chondrocytic differentiation of human bone marrow derived mesenchymal stem cells under the same 3-dimensional conditions. [Tissue Eng Part A]

Human Adipose-Derived Stromal Cells Efficiently Support Hematopoiesis In Vitro and In Vivo: A Key Step for Therapeutic Studies
In this manuscript, researchers show that human adipose-derived stromal cells cultured with GMP medium, maintain in vitro human early and committed hematopoietic progenitors and support their complete differentiation towards myeloid and lymphoid lineages. [Stem Cells Dev]

Human Mesenchymal Stem Cells from Adipose Tissue and Amnion Influence T-Cells Depending on Stimulation Method and Presence of Other Immune Cells
Researchers determined the influence of IFN-gamma on human adipose derived stem cells and human amniotic mesenchymal stromal cells. [Stem Cells Dev]

Hepatogenic Engineering from Human Bone Marrow Mesenchymal Stem Cells in Porous Polylactic Glycolic Acid Scaffolds Under Perfusion Culture
Researchers used mesenchymal stem cells from human bone marrow (hMSCs) as the seeding cells to investigate the potential of hepatocytic differentiation of hMSCs in porous polylactic glycolic acid scaffolds under perfusion induction. [J Tissue Eng Regen Med]

Interaction between Adipose Tissue Stromal Cells and Gastric Cancer Cells In Vitro
Adipose tissue stromal cells (ATSCs), which include mesenchymal stem cells (MSCs), seem critical for the progression of gastric cancer but their interaction with the cancer cells is unknown. Researchers demonstrated an interaction between these cells, using immunohistochemistry, Western blot and the collagen gel invasion assay system, in which the adenocarcinoma cells (well and poorly differentiated types, MKN28 and MKN45, respectively) were cultured on a ATSC-embedded or ATSC-non-embedded gel. [Cell Tissue Res]

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UMass, U.K. Stem Cell Banks Sign Agreement
As part of Gov. Deval Patrick’s Innovation Economy Partnership Mission 2011, global leaders in the field of stem cell banking and characterization—the University of Massachusetts Human Stem Cell Bank and Registry and the United Kingdom Stem Cell Bank—signed an agreement to share best practices for stem cell banking and to collaborate on standards for stem cell line characterization, production and distribution in the U.S. and U.K. [The University of Massachusetts Medical School Press Release]

National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

NEW Stem Cells in Development and Disease
Sept 11-14, 2011
Berlin, Germany

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Postdoctoral Position – Scientist (Helmholtz Zentrum München – German Research Center for Environmental Health – Helmholtz Association)

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