Interleukin-17 Enhances Immunosuppression by Mesenchymal Stem Cells Scientists examined the effect of interleukin-17 (IL-17) on mesenchymal stem cells (MSCs) under the influence of inflammatory cytokines. Ironically, IL-17 dramatically enhanced the immunosuppressive effect of MSCs induced by interferon-γ and tumor necrosis factor-α, revealing a novel role of IL-17 in immunosuppression. [Cell Death Differ] Full Article Bone Marrow-Derived Clonal Mesenchymal Stem Cells Inhibit Ovalbumin-Induced Atopic Dermatitis Using an ovalbumin-induced atopic dermatitis (AD) mouse model, researchers investigated whether mesenchymal stem cells (MSCs) can be used as therapeutics in AD. Clonal MSCs suppressed both T- and B-cell activation. [Cell Death Dis] Full Article Inhibition of B-Cell Proliferation and Antibody Production by Mesenchymal Stromal Cells Is Mediated by T Cells Scientists show that, both in healthy subjects and in patients, in vitro B-cell proliferation, plasma-cell differentiation and antibody production are inhibited by bone marrow-derived mesenchymal stromal cells (MSCs) when peripheral blood lymphocytes are stimulated with CpG, but not when sorted B cells are cultured with MSCs+CpG. [Stem Cells Dev] Abstract Cell-Viability and Chondrogenic Differentiation Capability of Human Mesenchymal Stem Cells after Iron-Labelling with Iron Sucrose Investigators determined effects on cell viability, traceability and chondrogenic differentiation of human mesenchymal stem cells after iron labelling with iron sucrose. [Stem Cells Dev] Abstract Autologous Platelet Rich Plasma: A Biological Supplement to Enhance Adipose-Derived Mesenchymal Stem Cell Expansion Adipose-derived mesenchymal stem cells (AT-MSCs) were cultured for ten days in media supplemented with different concentrations of autologous non-activated or thrombin-activated platelet-rich plasma. AT-MSC proliferation, cell phenotype, and multipotency capacity and chromosome stability were assessed and compared to AT-MSCs expanded in a classical medium supplemented with 10% of fetal bovine serum. [Tissue Eng Part C Methods] Abstract Expansion of Human Mesenchymal Stromal Cells from Fresh Bone Marrow in a 3D Scaffold-Based System under Direct Perfusion Scientists demonstrate that conventional monolayer culture on plastic dish mesenchymal stromal/stem cell expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells within three dimensional (3D) porous scaffolds in a perfusion-based bioreactor system. [PLoS One] Full Article Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells on Poly(L-Lactide) Nanofibrous Scaffolds Containing Carbon Nanomaterials Carbon nanomaterials (CNM) were incorporated into nanofibrous poly(L-lactic acid) scaffolds by thermal induced phase separation. The CNM-containing composite nanofibrous scaffolds were biologically evaluated by both in vitro co-culture of bone mesenchymal stem cells and in vivo implantation. [J Biomed Mater Res A] Abstract Overexpression of TGF-β1 Enhances Chondrogenic Differentiation and Proliferation of Human Synovium-Derived Stem Cells Scientists investigated the effect of continuous transforming growth factor (TGF)-β1 overexpression in human synovium-derived mesenchymal stem cells (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses encoding TGF-β1. [Biochem Biophys Res Commun] Abstract Neural Differentiation of Mesenchymal Stem Cells Influences Their Chemotactic Responses to Stromal Cell-Derived Factor-1α Researchers demonstrated that mesenchymal stem cells in varying neural differentiation states display various chemotactic responses to stromal cell-derived factor-1α. [Cell Mol Neurobiol] Abstract Frozen Adipose-Derived Mesenchymal Stem Cells Maintain High Capability to Grow and Differentiate Scientists show that adipose-derived stromal cells in serum free media can easily be frozen and stored in liquid nitrogen with retention of 85% of cell viability and 180,890 cell/g yield plus normal proliferative capacity and differentiation potential compared with fresh controls. [Cryobiology] Full Article A Novel Albumin-Based Tissue Scaffold for Autogenic Tissue Engineering Applications Using a culture of human mesenchymal stem cells (MSCs) as a model, scientists showed that MSCs can be seeded and grown in the albumin tissue scaffold. Furthermore, the albumin tissue scaffold can support the long-term osteogenic differentiation of MSCs. [Sci Rep] Full Article |