Mesenchymal Cell News Volume 6.31 | Aug 19 2014

Combinatorial Gene Therapy Accelerates Bone Regeneration: Non-Viral Dual Delivery of VEGF and BMP2 in a Collagen-Nanohydroxyapatite Scaffold
Vascularization and bone repair are accelerated by a series of gene-activated scaffolds delivering both an angiogenic and an osteogenic gene. Stem cell-mediated osteogenesis in vitro, in addition to increased vascularization and bone repair by host cells in vivo, is enhanced using all systems while the use of the nanohydroxyapatite vector to deliver both genes markedly enhances bone healing. [Adv Healthc Mater] Abstract

Combination of ADMSCs and Chondrocytes Reduces Hypertrophy and Improves the Functional Properties of Osteoarthritic Cartilage
Scientists evaluated the therapeutic efficacy of ADMSCs in combination with chondrocytes in counteracting oxidative stress in chondrocytes in vitro and in rat model of osteoarthritis. [Osteoarthritis Cartilage] Abstract

ABCG2^pos Lung Mesenchymal Stem Cells Are a Novel Pericyte Subpopulation that Contributes to Fibrotic Remodeling
Resident lung mesenchymal stem cells were quantified in lung samples from control subjects and pulmonary fibrosis (PF) patients. ABCG2^pos cell numbers were decreased in human PF and interstitial lung disease compared to control samples. [Am J Physiol Cell Physiol] Abstract

The Effect of Lentivirus-Mediated PSPN Genetic Engineering Bone Marrow Mesenchymal Stem Cells on Parkinson’s Disease Rat Model
Researchers assessed the potential therapeutic function of gene-modified mesenchymal stem cells (MSCs)-Lv-persephin (PSPN)-MSCs in 6-OHDA-induced Parkinson’s disease rat models. They worked on the isolation, purification, identification and amplification of MSCs in vitro. [PLoS One] Full Article

Human Adipose-Derived Mesenchymal Stem Cells Improve Motor Functions and Are Neuroprotective in the 6-Hydroxydopamine-Rat Model for Parkinson’s Disease when Cultured in Monolayer Cultures but Suppress Hippocampal Neurogenesis and Hippocampal Memory Function when Cultured in Spheroids
Researchers compared monolayer-cultured and spheroid mesenchymal stem cells following transplantation into the substantia nigra of 6-OHDA lesioned rats regarding effects on the local microenvironment, degeneration of dopaminergic neurons, neurogenesis in the hippocampal dentate gyrus as well as motor and memory function in the 6-OHDA-rat model for Parkinson´s disease. [Stem Cell Rev] Abstract

Downregulated CXCL12 Expression in Mesenchymal Stem Cells Associated with Severe Aplastic Anemia in Children
Scientists studied whether aberrant gene expression could be found in mesenchymal stem cells (MSCs) from children with severe aplastic anemia (SAA). Using microarray analysis, two different patterns of global gene expression were detected in the SAA MSCs. [Ann Hematol] Abstract

Sox2 Is a Potent Inhibitor of Osteogenic and Adipogenic Differentiation in Human Mesenchymal Stem Cells
Researchers overexpressed Sox2 in immortalized human mesenchymal stem cells by lentiviral gene transfer. Sox2 overexpression significantly reduced the osteogenic and adipogenic differentiation potentials. [Cell Reprogram] Abstract

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells
An oligopeptide synthesized derived from enamel matrix derivative was evaluated for whether it contributes to periodontal tissue regeneration. Scientists investigated the effects of the synthetic peptide on cell proliferation and osteoblast differentiation of human mesenchymal stem cells, which are involved in tissue regeneration. [Int J Mol Sci] Abstract | Download Full Article

Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells
Scientists reprogrammed mesenchymal stromal cells (MSCs) into induced pluripotent stem cells (iPSCs), which were subsequently redifferentiated toward MSCs. These iPS-MSCs revealed similar morphology, immunophenotype, in vitro differentiation potential, and gene expression profiles as primary MSCs. [Stem Cell Rep] Full Article | Graphical Abstract

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FDA Clears Mayo Clinic for Manufacture of NurOwn and Participation in BrainStorm’s Phase II ALS Trial
BrainStorm Cell Therapeutics announced that the U.S. Food and Drug Administration (FDA) has cleared the Mayo Clinic Human Cellular Therapy Laboratory in Rochester, Minn. for production of NurOwn™, BrainStorm’s proprietary autologous mesenchymal stem cells secreting neurotrophic factors. [BrainStorm Cell Therapeutics] Press Release

Stem Cell Therapy: FDA Regulatory Science Aims to Facilitate Development of Safe and Effective Regenerative Medicine Products
The FDA’s Center for Biologics Evaluation and Research assembled seven of its laboratories into a consortium to develop tests and techniques that will help answer questions as mesenchymal stem cell related products move through the development process. [U.S. Food and Drug Administration (FDA)] Article

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Postdoctoral Fellow – Intestinal Immunology (BSRC Fleming)

Postdoctoral Position – Biomaterials/Cartilage Regeneration (Peking University)

Cell Biology Technician (University of Pittsburgh School of Medicine)

Postdoctoral Fellowship – Developing Applications of Mesenchymal Stromal/Stem Cells in Hematopoietic Cell Transplantation (The Research Institute at Nationwide Children’s Hospital)

Postdoctoral Positions – Tissue Engineering and Regenerative Medicine (University of the Andes)

Technical Account Manager (GlobalStem)

PhD Position – Pericyte Function and Dysfunction in CNS Disease (Lund University)

Scientist – Mesenchymal Stem and Progenitor Cell Biology (STEMCELL Technologies Inc.)

Scientist – Myogenic Progenitor Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

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