Mesenchymal Cell News Volume 6.35 | Sep 16 2014

Scaffold Mean Pore Size Influences Mesenchymal Stem Cell Chondrogenic Differentiation and Matrix Deposition
Scientists investigated whether scaffold mean pore sizes affects mesenchymal stem cell initial attachment, morphology, chondrogenic gene expression and cartilage-like matrix deposition. [Tissue Eng Part A] Abstract

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable TIPS Microspheres
Scientists report the use of highly porous microcarriers produced using thermally induced phase separation (TIPS) to expand and differentiate adipose-derived mesenchymal stem cells into smooth muscle-like cells in a format that requires minimal manipulation before clinical delivery. [Tissue Eng Part C Methods] Abstract

Gene Expression Profile Analysis of Human Mesenchymal Stem Cells from Herniated and Degenerated Intervertebral Discs Reveals Different Expression of Osteopontin
Researchers present a characterization of mesenchymal stem cells (MSCs) isolated from clinically-categorized herniated (H-IVD) and degenerated (D-IVD) disc samples. H-IVD-MSCs and D-IVD-MSCs showed multipotent mesenchymal differentiation ability, expressing positivity for adipogenic, osteogenic, and chondrogenic markers with an immunophenotypical profile representative of MSCs. [Stem Cells Dev] Abstract

Novel Hedgehog Agonists Promote Osteoblast Differentiation in Mesenchymal Stem Cells
The authors demonstrate that hedgehog-Ag 1.3 and 1.7 strongly activate the expression of endogenous Gli1 and promote osteoblast differentiation in the mesenchymal stem cell line C3H10T1/2. [J Cell Physiol] Abstract

Decreased SIRT3 in Aged Human Mesenchymal Stromal/Stem Cells Increases Cellular Susceptibility to Oxidative Stress
Researchers show that severe oxidative stress reduces sirtuin3 (SIRT3) levels in young human mesenchymal stromal/stem cells (hMSCs). Overexpression of SIRT3 improved hMSCs resistance to the detrimental effects of oxidative stress. [J Cell Mol Med] Full Article

Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix
Researchers investigated the effects of negative pressure wound therapy on rat periosteum-derived mesenchymal stem cells proliferation and osteoblastic differentiation in a 3D fibrin matrix. [PLoS One]
Full Article

Mangiferin Reduces the Inhibition of Chondrogenic Differentiation by IL-1β in Mesenchymal Stem Cells from Subchondral Bone and Targets Multiple Aspects of the Smad and SOX9 Pathways
Scientists determined the effect of mangiferin on chondrogenic differentiation in IL-1β-stimulated mesenchymal stem cells from subchondral bone and to explore the mechanisms underlying these effects. [Int J Mol Sci] Abstract | Download Full Article

The Influence of IL-10 and TNFα on Chondrogenesis of Human Mesenchymal Stromal Cells in Three-Dimensional Cultures
Scientists determined the effectiveness of two three-dimensional (3D) culture systems for chondrogenic mesenchymal stromal cell (MSC) differentiation in comparison to primary chondrocytes and to assess the effect of Interleukin (IL)-10 and Tumor Necrosis Factor (TNF)α on chondrogenesis by MSCs in 3D high-density culture. [Int J Mol Sci] Abstract | Download Full Article

Human Bone Marrow Stromal Cells Lose Immunosuppressive and Anti-Inflammatory Properties upon Oncogenic Transformation
Using sequentially mutated human bone marrow stromal cells (hBMSCs) and spontaneously transformed hBMSCs, scientists report that, upon oncogenic transformation, hBMSCs lose immunosuppressive and anti-inflammatory properties in vitro and in vivo. [Stem Cell Rep] Full Article

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MEDIPOST America Clears Phase I/II IND for the U.S. Clinical Trial on Stem Cell Drug for Lung
MEDIPOST America Inc. announced the US FDA approval of Phase I/II clinical trial for PNEUMOSTEM^®. PNEUMOSTEM^® is made from allogeneic human umbilical cord blood-derived mesenchymal stem cells, and is expected to have effect on regenerating the lung tissue and improving inflammatory responses in premature infants. [MEDIPOST America Inc. (Business Wire)] Press Release

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NEW Junior Scientist – Multiple Myeloma (University Clinic Hamburg-Eppendorf)

NEW Postdoctoral Position – Cardiopulmonary Molecular Biology (Hannover Medical School)

Mesenchymal Stem/Stromal Cells Characterization (INSERM)

Mesenchymal Stromal/Stem Cell Scientist (UHN Arthritis Program)

Researcher – Phenotypic and Functional Characterization of Adult Stem/Stromal Cells from Bone Marrow and Adipose Tissue (Etablissement Français du Sang Pyrénées-Méditerranée)

Postdoctoral Position – Biomaterials/Cartilage Regeneration (Peking University)

Postdoctoral Fellowship – Developing Applications of Mesenchymal Stromal/Stem Cells in Hematopoietic Cell Transplantation (The Research Institute at Nationwide Children’s Hospital)

Scientist – Mesenchymal Stem and Progenitor Cell Biology (STEMCELL Technologies Inc.)

Scientist – Myogenic Progenitor Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

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