Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1 Similar to neural epidermal growth factor-like like protein (NELL)-1810, NELL-1570 induced mesenchymal stem cell (MSC) osteogenic differentiation. In addition, NELL-1570 significantly stimulated MSC proliferation in multiple MSC-like populations such as murine C3H10T1/2 MSC cell line, mouse primary MSCs, and perivascular stem cells. [Stem Cells] Abstract Coordinate Expansion of Murine Hematopoietic and Mesenchymal Stem Cell Compartments by SHIPi Researchers show that treatment of mice with aminosteroid inhibitors of SHIP1 (SHIPi) more than doubles the size of the adult mesenchymal stem cell compartment while simultaneously expanding the hematopoietic stem cell pool six-fold. [Stem Cells] Abstract Mesenchymal Stem Cells Correct Inappropriate Epithelial-Mesenchyme Relation in Pulmonary Fibrosis Using Stanniocalcin-1 Researchers show that mesenchymal stem cells can correct the inadequate-communication between epithelial and mesenchymal cells through stanniocalcin-1 secretion in a bleomycin-induced idiopathic pulmonary fibrosis model. [Mol Ther] Abstract Combination Therapy of Mesenchymal Stem Cells and Serelaxin Effectively Attenuates Renal Fibrosis in Obstructive Nephropathy Scientists tested the novel combination of mesenchymal stem cells with the antifibrotic serelaxin to repair and protect the kidney seven days post-unilateral ureteral obstruction, when fibrosis is established. [FASEB J] Abstract Mesenchymal Stem Cells and Ligand Incorporation in Biomimetic Poly(Ethylene Glycol) Hydrogels Significantly Improve Insulin Secretion from Pancreatic Islets Rat bone marrow-derived mesenchymal stem cells (MSCs) were encapsulated within synthetic poly(ethylene glycol) hydrogel, and cell viability and apoptosis within this 3D environment was examined in detail. ATP content and caspase-3 activity of encapsulated MSCs showed that fibronectin-derived RGDS, laminin-derived IKVAV and/or insulinotropic glucagon-like peptide were required to maintain MSC survival. [J Tissue Eng Regen Med] Abstract Mesenchymal Stem Cells Support Neuronal Fiber Growth in an Organotypic Brain Slice Co-Culture Model The impact of different isolation procedures on the functional and regenerative characteristics of mesenchymal stem cell (MSC) populations has not been studied thoroughly. To quantify these differences, scientists directly compared classically isolated bulk bone marrow-derived MSCs to the subpopulation Sca-1+Lin–CD45–-derived MSCs, isolated by FACS from bulk bone marrow-derived-cell suspensions. [Stem Cells Dev] Abstract The Dose-Effect Relationship between the Seeding Quantity of Human Marrow Mesenchymal Stem Cells and In Vivo Tissue Engineered Bone Yield Researchers report a dose-effect relationship of the quantity of seeded cell with in vivo bone yield, and the required quantity of human bone marrow mesenchymal stem cells for the effective, stable bone formation of tissue engineered bone. [Cell Transplant] Full Article Identification of Regulatory Factors for Mesenchymal Stem Cell-Derived Salivary Epithelial Cells in a Co-Culture System Bone marrow-derived mesenchymal stem cells were co-cultured with primary salivary epithelial cells from C57BL/6 mice. Co-cultured bone marrow-derived mesenchymal stem cells clearly resembled salivary epithelial cells, as confirmed by strong expression of salivary gland epithelial cell-specific markers, such as alpha-amylase, muscarinic type 3 receptor, aquaporin-5, and cytokeratin 19. [PLoS One] Full Article Mesenchymal Stem Cells Promote Epithelial to Mesenchymal Transition and Metastasis in Gastric Cancer through Paracrine Cues and Close Physical Contact Cancer cells acquired an “activated” carcinoma-associated fibroblasts phenotype after being in close contact with mesenchymal stem cells and enhancing tumor metastasis and growth in vivo. [J Cell Biochem] Abstract Pim-1 Kinase Cooperates with Serum Signals Supporting Mesenchymal Stem Cell Propagation Using genetically modified ovine mesenchymal stem cells (MSCs) that constitutively overexpressed Pim-1, researchers evaluated the impact of elevated Pim-1 activity on the proliferation, survival, and differentiation of MSCs in culture. [Cells Tissues Organs] Abstract Constitution of Fibrin-Based Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes Researchers hypothesized that adipose-derived mesenchymal stem cells may be directed to epidermal lineage in vitro on a specifically designed biomimetic and biodegradable niche. Cells were seeded on the test niche constituted with fibrin, fibronectin, gelatin, hyaluronic acid, laminin V, platelet growth factor, and epidermal growth factor in the presence of cell-specific differentiation medium. [Biores Open Access] Full Article |