Mesenchymal Cell News Volume 7.08 | Mar 3 2015

De-Regulated Expression of the BRG1 Chromatin Remodeling Factor in Bone Marrow Mesenchymal Stromal Cells Induces Senescence Associated with the Silencing of NANOG and Changes in the Levels of Chromatin Proteins
Researchers analyzed the effect of the de-regulation of the SWI/SNF chromatin remodeling complex in mesenchymal stromal cells through the silencing and up-regulation of BRG1, which is the ATPase subunit of the complex. [Cell Cycle] Abstract

Expansion, Harvest and Cryopreservation of Human Mesenchymal Stem Cells in a Serum-Free Microcarrier Process
Researchers ran a pilot study in the systematic development of serum-free human mesenchymal stem cell (hMSC) manufacturing process. Human bone-marrow derived hMSCs were expanded on fibronectin-coated, non-porous plastic microcarriers in 100 mL stirred spinner flasks in serum-free medium. [Biotechnol Bioeng] Abstract

Platelet-Derived Growth Factor-BB Enhances MSC-Mediated Cardioprotection via Suppression of miR-320 Expression
Human mesenchymal stem cells (MSCs), with or without PDGF preconditioning, were infused into the coronary circulation of isolated mouse hearts. The hearts that received PDGF-treated MSCs exhibited a greater functional recovery compared with naïve MSC-infused hearts, following ischemia/reperfusion injury. [Am J Physiol Heart Circ Physiol] Abstract

Autophagy and Mitochondrial Remodeling in Mouse Mesenchymal Stromal Cells Challenged with Staphylococcus epidermidis
Scientists demonstrated that in vitro challenge of mesenchymal stromal cells, i.e. colony-forming unit fibroblasts, with S. epidermidis can activate the autophagy pathway to execute antibacterial defense response, and that homoeostatic shift because of the bacteria-induced stress includes the mitochondrial remodeling and sequestration of compromised organelles via mitophagy. [J Cell Mol Med] Abstract

Adipose-Derived Mesenchymal Stem Cell Administration Does Not Improve Corneal Graft Survival Outcome
The effect of local and systemic injections of mesenchymal stem cells derived from adipose tissue into rabbit models of corneal allograft rejection with either normal-risk or high-risk vascularized corneal beds was investigated. [PLoS One] Full Article

Thermally Labile Components of Aqueous Humor Potently Induce Osteogenic Potential in Adipose-Derived Mesenchymal Stem Cells
Scientists cultured adipose-derived mesenchymal stem cells in the presence of 0, 5, 10, and 20% aqueous humor (AH) and assayed them for alkaline phosphatase (ALP) activity. They found ALP activity correlates with increasing AH concentrations from 5 to 20%, and that longer treatments result in increased ALP activity. [Exp Eye Res] Abstract

Generation of Insulin-Producing Cells from C3H10T1/2 Mesenchymal Progenitor Cells
Researchers investigated and compared the in vitro differentiation of rat mesenchymal stem cells and C3H10T1/2 cells into insulin-producing cells. These differentiated cells were transplanted into streptozotocin-induced diabetic mice and their biological functions were tested in vivo. [Gene] Abstract

Long-Term Expansion, Enhanced Chondrogenic Potential, and Suppression of Endochondral Ossification of Adult Human MSCs via WNT Signaling Modulation
Scientists report that the signaling protein WNT3A, in combination with FGF2, supports long-term expansion of human bone marrow-derived mesenchymal stem cells (MSCs). [Stem Cell Rep] Full Article

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Cynata Achieves Major Stem Cell Manufacturing Milestone
Australian stem cell and regenerative medicine company Cynata Therapeutics has achieved a world first breakthrough in the manufacture of stem cells and is now set to scale up manufacturing of its mesenchymal stem cells for therapeutic use. [Cynata Therapeutics (PR Newswire Association LLC)] Press Release

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NEW Postdoctoral Position – Pluripotent Stem Cells for Regenerative Medicine (Cells for Cells)

Research Associate: Mesenchymal (STEMCELL Technologies Inc.)

Scientist – Myogenic Progenitor Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

PhD Positions – Cartilage Regeneration in Joints and Intervertebral Disc Diseases (University Medical Center Utrecht)

Instructor – Regulation of Stem Cell Differentiation in Vascular Disease (The University of Alabama at Birmingham)

Director – Vector Production (Sangamo BioSciences, Inc.)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

Postdoctoral Position – Morphogenetic Signaling Pathways in Development and Disease (University of Rochester Medical Center)

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