Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration Chemical inhibition of mTORC1 with rapamycin substantially suppressed fluocinolone acetonide effect, and inhibition of AKT completely repressed chondrogenesis of human bone marrow-derived mesenchymal stem cells. [J Bone Miner Res] Abstract Combinatorial Effect of Substratum Properties on Mesenchymal Stem Cell Sheet Engineering and Subsequent Multi-Lineage Differentiation Polydimethylsiloxane of different combinatorial substratum properties were created, on which human bone marrow derived mesenchymal stem cells were cultured to form cell sheets with their multipotency evaluated after induced differentiation. [Acta Biomater] Abstract Protein Kinase CK2 Is Necessary for the Adipogenic Differentiation of Human Mesenchymal Stem Cells The authors analyzed CK2 during adipogenic differentiation of human mesenchymal stem cells. With progress of the differentiation CK2 protein level and the kinase activity decreased. [Biochim Biophys Acta] Abstract Paracrine Effects of Hematopoietic Cells on Human Mesenchymal Stem Cells Scientists assessed the paracrine effects of extrinsic factors from hematopoietic cells on human mesenchymal stem cells (MSCs). They showed that hematopoietic cells stimulate proliferation, osteoblast differentiation and inhibit senescence of MSCs. [Sci Rep] Full Article High-Throughput Screening of Surface Marker Expression on Undifferentiated and Differentiated Human Adipose-Derived Stromal Cells By comprehensively characterizing the surface marker profile of undifferentiated human adipose-derived stromal cells (hASCs) using flow cytometry, researchers gained novel insight into the heterogeneity underlying protein expression on the surface of cultured undifferentiated hASCs across different donors. [Tissue Eng Part A] Abstract Yes-Associated Protein (YAP) Is a Negative Regulator of Chondrogenesis in Mesenchymal Stem Cells Scientists investigated whether the transcriptional co-factor YAP regulates chondrogenic differentiation of mesenchymal stromal/stem cells (MSCs). YAP, but not transcriptional co-activator with PDZ-binding motif, was downregulated during in vitro chondrogenesis of human MSCs. [Arthritis Res Ther] Full Article Effect of TGF-β1 Stimulation on the Secretome of Human Adipose-Derived Mesenchymal Stromal Cells Transforming growth factor-β1 (TGF-β1) expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, researchers analyzed the effect of TGF-β1 on the secretion by adipose-derived mesenchymal stromal cells of a panel of 80 cytokines/chemokines using an antibody array. [Stem Cells Transl Med] Abstract Comparative Analysis of Neural Differentiation Potential in Human Mesenchymal Stem Cells Derived from Chorion and Adult Bone Marrow Investigators isolated and cultured chorion mesenchymal stem cells (C-MSCs) and compared their proliferative capacity, multipotency, and neural differentiation ability with bone marrow (BM)-MSCs. C-MSCs showed a higher proliferative capacity compared with BM-MSCs. [Cell Tissue Res] Abstract Clinically Applicable Human Adipose Tissue-Derived Mesenchymal Stem Cells Delivering Therapeutic Genes to Brainstem Gliomas In a good manufacturing practice facility, researchers established clinically applicable human adipose-derived mesenchymal stem cells expressing therapeutic genes and investigated their therapeutic efficacy against brainstem glioma in mice. [Cancer Gene Ther] Abstract Human Adipose-Derived Mesenchymal Progenitor Cells Engraft into Rabbit Articular Cartilage Scientists investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells promoted articular cartilage repair in rabbit osteoarthritis model and engrafted into rabbit articular cartilage. [Int J Mol Sci] Abstract | Download Full Article Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells Researchers investigated whether incorporation of α-smooth muscle actin (α-SMA) into contractile stress fibers regulates human mesenchymal stromal cell (MSC) fate. Sorted α-SMA-positive MSCs exhibited high contractile activity, low clonogenicity, and differentiation potential limited to osteogenesis. [Stem Cell Rep] Full Article | Graphical Abstract |